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- W2066408525 abstract "Various recombinant Hansenula polymorpha strains were developed and compared for their level of expression of the anticoagulant hirudin. H. polymorpha DL1-57 harboring an autonomously replicating sequence, HARS36, efficiently expressed the gene for recombinant hirudin. The effect of methanol oxidase (MOX) on the expression of the hirudin gene in H. polymorpha DL1-57 was studied, and the fermentation strategies coupled with the MOX activity and an antioxidant, tocopherol, were also examined." @default.
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- W2066408525 date "1998-08-01" @default.
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- W2066408525 title "Production of recombinant hirudin in Hansenula polymorpha : variation of gene expression level depends on methanol oxidase and fermentation strategies" @default.
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- W2066408525 doi "https://doi.org/10.1038/sj.jim.2900545" @default.
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