Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066410930> ?p ?o ?g. }
- W2066410930 abstract "Epithelial ovarian cancer (EOC) is the most common cause of gynecological malignancy-related mortality. Ovarian clear cell carcinoma (CCC) has unique clinical characteristics and behaviors that differ from other histological types of EOC, including a frequent association with endometriosis and a highly chemoresistant nature, resulting in poor prognosis. However, factors underlying its malignant behavior are still poorly understood. Aberrant expression of microRNAs has been shown to be involved in oncogenesis, and microRNA-21 (miR-21) is frequently overexpressed in many types of cancers. The aim of this study was to investigate the role of miR-21 in 17q23-25 amplification associated with CCC oncogenesis. We identified 17q23-25 copy number aberrations among 28 primary CCC tumors by using a comparative genomic hybridization method. Next, we measured expression levels of the candidate target genes, miR-21 and PPM1D, for 17q23-25 amplification by real-time RT-PCR analysis and compared those data with copy number status and clinicopathological features. In addition, immunohistochemical analysis of PTEN (a potential target of miR-21) was performed using the same primary CCC cases. We investigated the biological significance of miR-21 overexpression in CCC using a loss-of-function antisense approach. 17q23-25 amplification with both miR-21 overexpression and PTEN protein loss was detected in 4/28 CCC cases (14.2%). The patients with 17q23-25 amplification had significantly shorter progression-free and overall survival than those without 17q23-25 amplification (log-rank test: p = 0.0496; p = 0.0469, respectively). A significant correlation was observed between miR-21 overexpression and endometriosis. Both PTEN mRNA and PTEN protein expression were increased by miR-21 knockdown in CCC cells. We also confirmed that miR-21 directly bound to the 3′-untranslated region of PTEN mRNA using a dual-luciferase reporter assay. MiR-21 is a possible driver gene other than PPM1D for 17q23-25 amplification in CCC. Aberrant expression of miR-21 by chromosomal amplification might play an important role in CCC carcinogenesis through the regulation of the PTEN tumor suppressor gene." @default.
- W2066410930 created "2016-06-24" @default.
- W2066410930 creator A5005443199 @default.
- W2066410930 creator A5017026237 @default.
- W2066410930 creator A5018586070 @default.
- W2066410930 creator A5031577921 @default.
- W2066410930 creator A5043800120 @default.
- W2066410930 creator A5045956623 @default.
- W2066410930 creator A5051077930 @default.
- W2066410930 creator A5063042904 @default.
- W2066410930 creator A5075947539 @default.
- W2066410930 date "2014-11-03" @default.
- W2066410930 modified "2023-09-27" @default.
- W2066410930 title "MicroRNA-21 is a candidate driver gene for 17q23-25 amplification in ovarian clear cell carcinoma" @default.
- W2066410930 cites W1758114794 @default.
- W2066410930 cites W1791183955 @default.
- W2066410930 cites W1969174225 @default.
- W2066410930 cites W1972160613 @default.
- W2066410930 cites W1975141727 @default.
- W2066410930 cites W1989125392 @default.
- W2066410930 cites W1993685841 @default.
- W2066410930 cites W2000093333 @default.
- W2066410930 cites W2006797972 @default.
- W2066410930 cites W2025423482 @default.
- W2066410930 cites W2032701173 @default.
- W2066410930 cites W2045065956 @default.
- W2066410930 cites W2048535908 @default.
- W2066410930 cites W2054388774 @default.
- W2066410930 cites W2066810125 @default.
- W2066410930 cites W2067841857 @default.
- W2066410930 cites W2096389012 @default.
- W2066410930 cites W2110632782 @default.
- W2066410930 cites W2130979840 @default.
- W2066410930 cites W2135810600 @default.
- W2066410930 cites W2148720931 @default.
- W2066410930 cites W2150536104 @default.
- W2066410930 cites W2152197423 @default.
- W2066410930 cites W2153672941 @default.
- W2066410930 cites W2158335713 @default.
- W2066410930 cites W2163476499 @default.
- W2066410930 cites W2167715370 @default.
- W2066410930 cites W3140139122 @default.
- W2066410930 doi "https://doi.org/10.1186/1471-2407-14-799" @default.
- W2066410930 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4289307" @default.
- W2066410930 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25366985" @default.
- W2066410930 hasPublicationYear "2014" @default.
- W2066410930 type Work @default.
- W2066410930 sameAs 2066410930 @default.
- W2066410930 citedByCount "31" @default.
- W2066410930 countsByYear W20664109302015 @default.
- W2066410930 countsByYear W20664109302016 @default.
- W2066410930 countsByYear W20664109302017 @default.
- W2066410930 countsByYear W20664109302018 @default.
- W2066410930 countsByYear W20664109302019 @default.
- W2066410930 countsByYear W20664109302020 @default.
- W2066410930 countsByYear W20664109302021 @default.
- W2066410930 countsByYear W20664109302022 @default.
- W2066410930 countsByYear W20664109302023 @default.
- W2066410930 crossrefType "journal-article" @default.
- W2066410930 hasAuthorship W2066410930A5005443199 @default.
- W2066410930 hasAuthorship W2066410930A5017026237 @default.
- W2066410930 hasAuthorship W2066410930A5018586070 @default.
- W2066410930 hasAuthorship W2066410930A5031577921 @default.
- W2066410930 hasAuthorship W2066410930A5043800120 @default.
- W2066410930 hasAuthorship W2066410930A5045956623 @default.
- W2066410930 hasAuthorship W2066410930A5051077930 @default.
- W2066410930 hasAuthorship W2066410930A5063042904 @default.
- W2066410930 hasAuthorship W2066410930A5075947539 @default.
- W2066410930 hasBestOaLocation W20664109301 @default.
- W2066410930 hasConcept C104317684 @default.
- W2066410930 hasConcept C121608353 @default.
- W2066410930 hasConcept C126322002 @default.
- W2066410930 hasConcept C143998085 @default.
- W2066410930 hasConcept C145059251 @default.
- W2066410930 hasConcept C190283241 @default.
- W2066410930 hasConcept C2777546739 @default.
- W2066410930 hasConcept C2777609662 @default.
- W2066410930 hasConcept C2779399171 @default.
- W2066410930 hasConcept C2780140570 @default.
- W2066410930 hasConcept C2780427987 @default.
- W2066410930 hasConcept C2781199814 @default.
- W2066410930 hasConcept C502942594 @default.
- W2066410930 hasConcept C54355233 @default.
- W2066410930 hasConcept C555283112 @default.
- W2066410930 hasConcept C71924100 @default.
- W2066410930 hasConcept C7602840 @default.
- W2066410930 hasConcept C86554907 @default.
- W2066410930 hasConcept C86803240 @default.
- W2066410930 hasConceptScore W2066410930C104317684 @default.
- W2066410930 hasConceptScore W2066410930C121608353 @default.
- W2066410930 hasConceptScore W2066410930C126322002 @default.
- W2066410930 hasConceptScore W2066410930C143998085 @default.
- W2066410930 hasConceptScore W2066410930C145059251 @default.
- W2066410930 hasConceptScore W2066410930C190283241 @default.
- W2066410930 hasConceptScore W2066410930C2777546739 @default.
- W2066410930 hasConceptScore W2066410930C2777609662 @default.
- W2066410930 hasConceptScore W2066410930C2779399171 @default.
- W2066410930 hasConceptScore W2066410930C2780140570 @default.
- W2066410930 hasConceptScore W2066410930C2780427987 @default.
- W2066410930 hasConceptScore W2066410930C2781199814 @default.