FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066496579> ?p ?o ?g. }

• W2066496579 endingPage "e28738" @default.
• W2066496579 startingPage "e28738" @default.
• W2066496579 abstract "It is unclear whether Streptococcus pneumoniae in biofilms are virulent and contribute to development of invasive pneumococcal disease (IPD). Using electron microscopy we confirmed the development of mature pneumococcal biofilms in a continuous-flow-through line model and determined that biofilm formation occurred in discrete stages with mature biofilms composed primarily of dead pneumococci. Challenge of mice with equal colony forming units of biofilm and planktonic pneumococci determined that biofilm bacteria were highly attenuated for invasive disease but not nasopharyngeal colonization. Biofilm pneumococci of numerous serotypes were hyper-adhesive and bound to A549 type II pneumocytes and Detroit 562 pharyngeal epithelial cells at levels 2 to 11-fold greater than planktonic counterparts. Using genomic microarrays we examined the pneumococcal transcriptome and determined that during biofilm formation S. pneumoniae down-regulated genes involved in protein synthesis, energy production, metabolism, capsular polysaccharide (CPS) production, and virulence. We confirmed these changes by measuring CPS by ELISA and immunoblotting for the toxin pneumolysin and the bacterial adhesins phosphorylcholine (ChoP), choline-binding protein A (CbpA), and Pneumococcal serine-rich repeat protein (PsrP). We conclude that biofilm pneumococci were avirulent due to reduced CPS and pneumolysin production along with increased ChoP, which is known to bind C-reactive protein and is opsonizing. Likewise, biofilm pneumococci were hyper-adhesive due to selection for the transparent phase variant, reduced CPS, and enhanced production of PsrP, CbpA, and ChoP. These studies suggest that biofilms do not directly contribute to development of IPD and may instead confer a quiescent mode of growth during colonization." @default.
• W2066496579 created "2016-06-24" @default.
• W2066496579 creator A5009841943 @default.
• W2066496579 creator A5012075335 @default.
• W2066496579 creator A5032463863 @default.
• W2066496579 creator A5039625819 @default.
• W2066496579 creator A5048555409 @default.
• W2066496579 creator A5058179549 @default.
• W2066496579 creator A5070096017 @default.
• W2066496579 creator A5084925508 @default.
• W2066496579 date "2011-12-08" @default.
• W2066496579 modified "2023-10-07" @default.
• W2066496579 title "Streptococcus pneumoniae in Biofilms Are Unable to Cause Invasive Disease Due to Altered Virulence Determinant Production" @default.
• W2066496579 cites W1845427115 @default.
• W2066496579 cites W1852799255 @default.
• W2066496579 cites W1963268238 @default.
• W2066496579 cites W1967386006 @default.
• W2066496579 cites W1969520497 @default.
• W2066496579 cites W1972775775 @default.
• W2066496579 cites W1973339314 @default.
• W2066496579 cites W1978465166 @default.
• W2066496579 cites W1993896162 @default.
• W2066496579 cites W1995170954 @default.
• W2066496579 cites W1996802863 @default.
• W2066496579 cites W2004218257 @default.
• W2066496579 cites W2005741947 @default.
• W2066496579 cites W2007296377 @default.
• W2066496579 cites W2014197969 @default.
• W2066496579 cites W2027773277 @default.
• W2066496579 cites W2034722272 @default.
• W2066496579 cites W2048834367 @default.
• W2066496579 cites W2050542389 @default.
• W2066496579 cites W2052643656 @default.
• W2066496579 cites W2054051063 @default.
• W2066496579 cites W2064259891 @default.
• W2066496579 cites W2070088956 @default.
• W2066496579 cites W2073407655 @default.
• W2066496579 cites W2078606044 @default.
• W2066496579 cites W2083367830 @default.
• W2066496579 cites W2083701603 @default.
• W2066496579 cites W2093717213 @default.
• W2066496579 cites W2098483219 @default.
• W2066496579 cites W2101203381 @default.
• W2066496579 cites W2107277218 @default.
• W2066496579 cites W2113326101 @default.
• W2066496579 cites W2117461422 @default.
• W2066496579 cites W2121369371 @default.
• W2066496579 cites W2122873066 @default.
• W2066496579 cites W2124219105 @default.
• W2066496579 cites W2125603952 @default.
• W2066496579 cites W2129333922 @default.
• W2066496579 cites W2129677884 @default.
• W2066496579 cites W2129920113 @default.
• W2066496579 cites W2130609362 @default.
• W2066496579 cites W2132979148 @default.
• W2066496579 cites W2133059828 @default.
• W2066496579 cites W2133579523 @default.
• W2066496579 cites W2135610044 @default.
• W2066496579 cites W2135789554 @default.
• W2066496579 cites W2139803866 @default.
• W2066496579 cites W2144240057 @default.
• W2066496579 cites W2144440398 @default.
• W2066496579 cites W2148637247 @default.
• W2066496579 cites W2148717264 @default.
• W2066496579 cites W2148756961 @default.
• W2066496579 cites W2153131694 @default.
• W2066496579 cites W2153736430 @default.
• W2066496579 cites W2156316660 @default.
• W2066496579 cites W2157999551 @default.
• W2066496579 cites W2159685144 @default.
• W2066496579 cites W2161725783 @default.
• W2066496579 cites W2162287173 @default.
• W2066496579 cites W2163774445 @default.
• W2066496579 cites W2165629047 @default.
• W2066496579 cites W2167221249 @default.
• W2066496579 cites W2168857380 @default.
• W2066496579 cites W2188055647 @default.
• W2066496579 cites W45534775 @default.
• W2066496579 cites W54930568 @default.
• W2066496579 doi "https://doi.org/10.1371/journal.pone.0028738" @default.
• W2066496579 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3234282" @default.
• W2066496579 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22174882" @default.
• W2066496579 hasPublicationYear "2011" @default.
• W2066496579 type Work @default.
• W2066496579 sameAs 2066496579 @default.
• W2066496579 citedByCount "103" @default.
• W2066496579 countsByYear W20664965792012 @default.
• W2066496579 countsByYear W20664965792013 @default.
• W2066496579 countsByYear W20664965792014 @default.
• W2066496579 countsByYear W20664965792015 @default.
• W2066496579 countsByYear W20664965792016 @default.
• W2066496579 countsByYear W20664965792017 @default.
• W2066496579 countsByYear W20664965792018 @default.
• W2066496579 countsByYear W20664965792019 @default.
• W2066496579 countsByYear W20664965792020 @default.
• W2066496579 countsByYear W20664965792021 @default.
• W2066496579 countsByYear W20664965792022 @default.
• W2066496579 countsByYear W20664965792023 @default.

• next