FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066577113> ?p ?o ?g. }

• W2066577113 endingPage "31615" @default.
• W2066577113 startingPage "31603" @default.
• W2066577113 abstract "Chitinases hydrolyze chitin, an insoluble linear polymer of N-acetyl-d-glucosamine (NAG)(n), into nutrient sources. Bacillus cereus NCTU2 chitinase (ChiNCTU2) predominantly produces chitobioses and belongs to glycoside hydrolase family 18. The crystal structure of wild-type ChiNCTU2 comprises only a catalytic domain, unlike other chitinases that are equipped with additional chitin binding and insertion domains to bind substrates into the active site. Lacking chitin binding and chitin insertion domains, ChiNCTU2 utilizes two dynamic loops (Gly-67-Thr-69 and Ile-106-Val-112) to interact with (NAG)(n), generating novel substrate binding and distortion for catalysis. Gln-109 is crucial for direct binding with substrates, leading to conformational changes of two loops with a maximum shift of ∼4.6 Å along the binding cleft. The structures of E145Q, E145Q/Y227F, and E145G/Y227F mutants complexed with (NAG)(n) reveal (NAG)(2), (NAG)(2), and (NAG)(4) in the active site, respectively, implying various stages of reaction: before hydrolysis, E145G/Y227F with (NAG)(4); in an intermediate state, E145Q/Y227F with a boat-form NAG at the -1 subsite, -1-(NAG); after hydrolysis, E145Q with a chair form -1-(NAG). Several residues were confirmed to play catalytic roles: Glu-145 in cleavage of the glycosidic bond between -1-(NAG) and +1-(NAG); Tyr-227 in the conformational change of -1-(NAG); Asp-143 and Gln-225 in stabilizing the conformation of -1-(NAG). Additionally, Glu-190 acts in the process of product release, and Tyr-193 coordinates with water for catalysis. Residues Asp-143, E145Q, Glu-190, and Tyr-193 exhibit multiple conformations for functions. The inhibitors zinc ions and cyclo-(l-His-l-Pro) are located at various positions and confirm the catalytic-site topology. Together with kinetics analyses of related mutants, the structures of ChiNCTU2 and its mutant complexes with (NAG)(n) provide new insights into its substrate binding and the mechanistic action." @default.
• W2066577113 created "2016-06-24" @default.
• W2066577113 creator A5004329931 @default.
• W2066577113 creator A5021267328 @default.
• W2066577113 creator A5025033339 @default.
• W2066577113 creator A5039192004 @default.
• W2066577113 creator A5043997420 @default.
• W2066577113 creator A5045573961 @default.
• W2066577113 creator A5063280655 @default.
• W2066577113 creator A5071614218 @default.
• W2066577113 creator A5071765104 @default.
• W2066577113 creator A5085395936 @default.
• W2066577113 creator A5085919623 @default.
• W2066577113 date "2010-10-01" @default.
• W2066577113 modified "2023-09-30" @default.
• W2066577113 title "Crystal Structures of Bacillus cereus NCTU2 Chitinase Complexes with Chitooligomers Reveal Novel Substrate Binding for Catalysis" @default.
• W2066577113 cites W1539796472 @default.
• W2066577113 cites W1546896179 @default.
• W2066577113 cites W1557004277 @default.
• W2066577113 cites W1830745998 @default.
• W2066577113 cites W1965277349 @default.
• W2066577113 cites W1968278804 @default.
• W2066577113 cites W1969215536 @default.
• W2066577113 cites W1974515964 @default.
• W2066577113 cites W1980701408 @default.
• W2066577113 cites W1984846491 @default.
• W2066577113 cites W1985751498 @default.
• W2066577113 cites W1986191025 @default.
• W2066577113 cites W1989430502 @default.
• W2066577113 cites W1989449470 @default.
• W2066577113 cites W1995017064 @default.
• W2066577113 cites W1996607909 @default.
• W2066577113 cites W2006256864 @default.
• W2066577113 cites W2008481402 @default.
• W2066577113 cites W2013083986 @default.
• W2066577113 cites W2014936174 @default.
• W2066577113 cites W2017071215 @default.
• W2066577113 cites W2017897589 @default.
• W2066577113 cites W2023301414 @default.
• W2066577113 cites W2025225233 @default.
• W2066577113 cites W2026995511 @default.
• W2066577113 cites W2035160545 @default.
• W2066577113 cites W2051504796 @default.
• W2066577113 cites W2062428389 @default.
• W2066577113 cites W2070695325 @default.
• W2066577113 cites W2080054817 @default.
• W2066577113 cites W2084575458 @default.
• W2066577113 cites W2086806574 @default.
• W2066577113 cites W2087082796 @default.
• W2066577113 cites W2092909113 @default.
• W2066577113 cites W2097493124 @default.
• W2066577113 cites W2098303032 @default.
• W2066577113 cites W2105738898 @default.
• W2066577113 cites W2107368146 @default.
• W2066577113 cites W2108072410 @default.
• W2066577113 cites W2108859348 @default.
• W2066577113 cites W2112185869 @default.
• W2066577113 cites W2112200653 @default.
• W2066577113 cites W2115317679 @default.
• W2066577113 cites W2116275717 @default.
• W2066577113 cites W2120641909 @default.
• W2066577113 cites W2127071742 @default.
• W2066577113 cites W2134194234 @default.
• W2066577113 cites W2143694829 @default.
• W2066577113 cites W2144081223 @default.
• W2066577113 cites W2144275021 @default.
• W2066577113 cites W2148377603 @default.
• W2066577113 cites W2155082155 @default.
• W2066577113 cites W2165600016 @default.
• W2066577113 cites W2168625934 @default.
• W2066577113 cites W2169919973 @default.
• W2066577113 cites W2266642515 @default.
• W2066577113 cites W2916193864 @default.
• W2066577113 cites W4243388922 @default.
• W2066577113 doi "https://doi.org/10.1074/jbc.m110.149310" @default.
• W2066577113 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2951234" @default.
• W2066577113 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20685646" @default.
• W2066577113 hasPublicationYear "2010" @default.
• W2066577113 type Work @default.
• W2066577113 sameAs 2066577113 @default.
• W2066577113 citedByCount "51" @default.
• W2066577113 countsByYear W20665771132012 @default.
• W2066577113 countsByYear W20665771132013 @default.
• W2066577113 countsByYear W20665771132014 @default.
• W2066577113 countsByYear W20665771132015 @default.
• W2066577113 countsByYear W20665771132016 @default.
• W2066577113 countsByYear W20665771132017 @default.
• W2066577113 countsByYear W20665771132018 @default.
• W2066577113 countsByYear W20665771132019 @default.
• W2066577113 countsByYear W20665771132020 @default.
• W2066577113 countsByYear W20665771132021 @default.
• W2066577113 countsByYear W20665771132023 @default.
• W2066577113 crossrefType "journal-article" @default.
• W2066577113 hasAuthorship W2066577113A5004329931 @default.
• W2066577113 hasAuthorship W2066577113A5021267328 @default.
• W2066577113 hasAuthorship W2066577113A5025033339 @default.
• W2066577113 hasAuthorship W2066577113A5039192004 @default.
• W2066577113 hasAuthorship W2066577113A5043997420 @default.

• next