Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066616401> ?p ?o ?g. }
- W2066616401 endingPage "280" @default.
- W2066616401 startingPage "274" @default.
- W2066616401 abstract "Abstract Most of lipases are in equilibrium between a majority inactive closed form and a minority active open form in aqueous media. Perhaps, a certain stabilization of these open forms of lipases could be achieved in the presence of cosolvents or surfactants in the reaction medium. Three commercial lipases were studied (from Thermomyces lanuginosa (TLL), Candida Antarctica fraction B (CALB) and Lecitase (LEC)). Different derivatives were tested: TLL and LEC were adsorbed on an anionic exchanger and their activity strongly depends on the equilibrium between their open and closed form and CALB was adsorbed on a hydrophobic support when the open form was already stabilized by the support. Derivatives ionically adsorbed were hyperactivated by surfactans as well as by cosolvents: the activity of LEC increased 12 times in the presence of 15–20% of ethanol. CALB adsorbed on hydrophobic supports was hardly hyperactivated and even it was inhibited. The modification of the rate of covalent modification of the catalytic Ser seems to confirm that the observed hyperactivations were due to a stabilization of the open form of the adsorbed lipases (TLL and LEC). The hydrolysis of sardine oil was also studied in the presence or absence of surfactants and cosolvents. An interesting improvement in the ability of derivatives to discriminate the release of eicosipentaenic acid (EPA) and docosahexaenicacid (DHA) was found." @default.
- W2066616401 created "2016-06-24" @default.
- W2066616401 creator A5002781426 @default.
- W2066616401 creator A5012665364 @default.
- W2066616401 creator A5041270570 @default.
- W2066616401 creator A5065677467 @default.
- W2066616401 creator A5066184946 @default.
- W2066616401 creator A5067470267 @default.
- W2066616401 creator A5069963901 @default.
- W2066616401 creator A5070874884 @default.
- W2066616401 creator A5077558844 @default.
- W2066616401 creator A5091623586 @default.
- W2066616401 date "2015-01-01" @default.
- W2066616401 modified "2023-10-08" @default.
- W2066616401 title "Modulation of the activity and selectivity of the immobilized lipases by surfactants and solvents" @default.
- W2066616401 cites W1965941994 @default.
- W2066616401 cites W1984063461 @default.
- W2066616401 cites W1988660541 @default.
- W2066616401 cites W1990458165 @default.
- W2066616401 cites W2002142735 @default.
- W2066616401 cites W2006591992 @default.
- W2066616401 cites W2015150213 @default.
- W2066616401 cites W2021050137 @default.
- W2066616401 cites W2035278817 @default.
- W2066616401 cites W2038003705 @default.
- W2066616401 cites W2040337511 @default.
- W2066616401 cites W2048830170 @default.
- W2066616401 cites W2079013207 @default.
- W2066616401 cites W2086864633 @default.
- W2066616401 cites W2095417541 @default.
- W2066616401 cites W2110419883 @default.
- W2066616401 cites W2111537738 @default.
- W2066616401 cites W2159580538 @default.
- W2066616401 cites W2161249744 @default.
- W2066616401 cites W2405221203 @default.
- W2066616401 doi "https://doi.org/10.1016/j.bej.2014.10.009" @default.
- W2066616401 hasPublicationYear "2015" @default.
- W2066616401 type Work @default.
- W2066616401 sameAs 2066616401 @default.
- W2066616401 citedByCount "42" @default.
- W2066616401 countsByYear W20666164012016 @default.
- W2066616401 countsByYear W20666164012017 @default.
- W2066616401 countsByYear W20666164012018 @default.
- W2066616401 countsByYear W20666164012019 @default.
- W2066616401 countsByYear W20666164012020 @default.
- W2066616401 countsByYear W20666164012021 @default.
- W2066616401 countsByYear W20666164012022 @default.
- W2066616401 countsByYear W20666164012023 @default.
- W2066616401 crossrefType "journal-article" @default.
- W2066616401 hasAuthorship W2066616401A5002781426 @default.
- W2066616401 hasAuthorship W2066616401A5012665364 @default.
- W2066616401 hasAuthorship W2066616401A5041270570 @default.
- W2066616401 hasAuthorship W2066616401A5065677467 @default.
- W2066616401 hasAuthorship W2066616401A5066184946 @default.
- W2066616401 hasAuthorship W2066616401A5067470267 @default.
- W2066616401 hasAuthorship W2066616401A5069963901 @default.
- W2066616401 hasAuthorship W2066616401A5070874884 @default.
- W2066616401 hasAuthorship W2066616401A5077558844 @default.
- W2066616401 hasAuthorship W2066616401A5091623586 @default.
- W2066616401 hasConcept C107038049 @default.
- W2066616401 hasConcept C118792377 @default.
- W2066616401 hasConcept C123079801 @default.
- W2066616401 hasConcept C138885662 @default.
- W2066616401 hasConcept C161790260 @default.
- W2066616401 hasConcept C178790620 @default.
- W2066616401 hasConcept C181199279 @default.
- W2066616401 hasConcept C185592680 @default.
- W2066616401 hasConcept C21951064 @default.
- W2066616401 hasConcept C2779697368 @default.
- W2066616401 hasConcept C43617362 @default.
- W2066616401 hasConceptScore W2066616401C107038049 @default.
- W2066616401 hasConceptScore W2066616401C118792377 @default.
- W2066616401 hasConceptScore W2066616401C123079801 @default.
- W2066616401 hasConceptScore W2066616401C138885662 @default.
- W2066616401 hasConceptScore W2066616401C161790260 @default.
- W2066616401 hasConceptScore W2066616401C178790620 @default.
- W2066616401 hasConceptScore W2066616401C181199279 @default.
- W2066616401 hasConceptScore W2066616401C185592680 @default.
- W2066616401 hasConceptScore W2066616401C21951064 @default.
- W2066616401 hasConceptScore W2066616401C2779697368 @default.
- W2066616401 hasConceptScore W2066616401C43617362 @default.
- W2066616401 hasFunder F4320320997 @default.
- W2066616401 hasLocation W20666164011 @default.
- W2066616401 hasOpenAccess W2066616401 @default.
- W2066616401 hasPrimaryLocation W20666164011 @default.
- W2066616401 hasRelatedWork W2021939235 @default.
- W2066616401 hasRelatedWork W2160162460 @default.
- W2066616401 hasRelatedWork W2370841964 @default.
- W2066616401 hasRelatedWork W2777174519 @default.
- W2066616401 hasRelatedWork W2950422474 @default.
- W2066616401 hasRelatedWork W2951652524 @default.
- W2066616401 hasRelatedWork W3005527239 @default.
- W2066616401 hasRelatedWork W3155051169 @default.
- W2066616401 hasRelatedWork W3200462162 @default.
- W2066616401 hasRelatedWork W4240339722 @default.
- W2066616401 hasVolume "93" @default.
- W2066616401 isParatext "false" @default.
- W2066616401 isRetracted "false" @default.