FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066617026> ?p ?o ?g. }

• W2066617026 endingPage "103" @default.
• W2066617026 startingPage "93" @default.
• W2066617026 abstract "We have constructed a cDNA library for the trimethylguanosine-capped small RNAs (sRNAs) in the acellular slime mold Physarum polycephalum. Capped sRNAs were purified from total cellular RNA of vegetative microplasmodia by preparative immunoprecipitation with anti-trimethylguanosine antibody. The purified RNA was analyzed by polyacrylamide gel electrophoresis. Approx. eleven different capped sRNAs were observed with a size range of 70-204 nucleotides (nt). Based on their approximate sizes, the presence of trimethylguanosine cap, and the presence of a lupus type-Sm antigen, molecules U1-U7 (excluding U3) were identified. Further confirmation of the identity of molecule U1a was established by Northern hybridization, U4a by colony hybridization, and U6 and U7a by direct chemical sequence analysis. Purified capped sRNAs were tailed with oligo(A), and inserted into oligo(dT)-tailed plasmid pCDV1. The cDNAs were used to transform Escherichia coli strain HB101. Approx. 1.9 X 10(5) ampicillin-resistant (ApR) transformants were obtained per microgram of tailed sRNA. Dot-blot hybridization, using Physarum RNA precipitated with anti-cap antibody as a probe, indicated that approx. 94% of the ApR colonies contained recombinant DNAs. The library was screened by colony hybridization using heterologous sRNA probes. Clones hybridizing with heterologous sRNAs U1, U2, U4 and U7 were each represented in the library in approximately the same frequency as their relative abundance in the Physarum sRNA population they were derived from. The insert of one Physarum U4 clone was sequenced and was found to have 57.1% homology with nt 1-91 of the published sequence for rat U4 RNA. A 12-nt 'functional' subdomain of the rat U4 molecule was 83.3% conserved in Physarum U4." @default.
• W2066617026 created "2016-06-24" @default.
• W2066617026 creator A5036229127 @default.
• W2066617026 creator A5038951708 @default.
• W2066617026 creator A5059677466 @default.
• W2066617026 creator A5062111821 @default.
• W2066617026 date "1987-01-01" @default.
• W2066617026 modified "2023-09-25" @default.
• W2066617026 title "A library of trimethylguanosine-capped small RNAS in Physarum polycephalum" @default.
• W2066617026 cites W1496392745 @default.
• W2066617026 cites W1550094295 @default.
• W2066617026 cites W1558516755 @default.
• W2066617026 cites W1584113545 @default.
• W2066617026 cites W1592715073 @default.
• W2066617026 cites W1823872220 @default.
• W2066617026 cites W1899701997 @default.
• W2066617026 cites W1918119607 @default.
• W2066617026 cites W1966694632 @default.
• W2066617026 cites W1971314065 @default.
• W2066617026 cites W1977468142 @default.
• W2066617026 cites W1982610079 @default.
• W2066617026 cites W1990823895 @default.
• W2066617026 cites W1990999940 @default.
• W2066617026 cites W1994261379 @default.
• W2066617026 cites W2003258975 @default.
• W2066617026 cites W2011974050 @default.
• W2066617026 cites W2012557167 @default.
• W2066617026 cites W2046110709 @default.
• W2066617026 cites W2050210314 @default.
• W2066617026 cites W2050971795 @default.
• W2066617026 cites W2058882288 @default.
• W2066617026 cites W2060244903 @default.
• W2066617026 cites W2066244683 @default.
• W2066617026 cites W2068744169 @default.
• W2066617026 cites W2069573006 @default.
• W2066617026 cites W2078119356 @default.
• W2066617026 cites W2089582966 @default.
• W2066617026 cites W2090314721 @default.
• W2066617026 cites W2091408625 @default.
• W2066617026 cites W2122071753 @default.
• W2066617026 cites W2154349481 @default.
• W2066617026 cites W2178757995 @default.
• W2066617026 cites W4213231968 @default.
• W2066617026 cites W4214897113 @default.
• W2066617026 doi "https://doi.org/10.1016/0378-1119(87)90351-9" @default.
• W2066617026 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2956157" @default.
• W2066617026 hasPublicationYear "1987" @default.
• W2066617026 type Work @default.
• W2066617026 sameAs 2066617026 @default.
• W2066617026 citedByCount "11" @default.
• W2066617026 countsByYear W20666170262014 @default.
• W2066617026 countsByYear W20666170262021 @default.
• W2066617026 crossrefType "journal-article" @default.
• W2066617026 hasAuthorship W2066617026A5036229127 @default.
• W2066617026 hasAuthorship W2066617026A5038951708 @default.
• W2066617026 hasAuthorship W2066617026A5059677466 @default.
• W2066617026 hasAuthorship W2066617026A5062111821 @default.
• W2066617026 hasConcept C104317684 @default.
• W2066617026 hasConcept C153911025 @default.
• W2066617026 hasConcept C187882448 @default.
• W2066617026 hasConcept C2778153493 @default.
• W2066617026 hasConcept C2780855919 @default.
• W2066617026 hasConcept C54355233 @default.
• W2066617026 hasConcept C55493867 @default.
• W2066617026 hasConcept C67705224 @default.
• W2066617026 hasConcept C86803240 @default.
• W2066617026 hasConceptScore W2066617026C104317684 @default.
• W2066617026 hasConceptScore W2066617026C153911025 @default.
• W2066617026 hasConceptScore W2066617026C187882448 @default.
• W2066617026 hasConceptScore W2066617026C2778153493 @default.
• W2066617026 hasConceptScore W2066617026C2780855919 @default.
• W2066617026 hasConceptScore W2066617026C54355233 @default.
• W2066617026 hasConceptScore W2066617026C55493867 @default.
• W2066617026 hasConceptScore W2066617026C67705224 @default.
• W2066617026 hasConceptScore W2066617026C86803240 @default.
• W2066617026 hasIssue "1" @default.
• W2066617026 hasLocation W20666170261 @default.
• W2066617026 hasLocation W20666170262 @default.
• W2066617026 hasOpenAccess W2066617026 @default.
• W2066617026 hasPrimaryLocation W20666170261 @default.
• W2066617026 hasRelatedWork W1664917681 @default.
• W2066617026 hasRelatedWork W1996686187 @default.
• W2066617026 hasRelatedWork W2033358430 @default.
• W2066617026 hasRelatedWork W2044948282 @default.
• W2066617026 hasRelatedWork W2086642386 @default.
• W2066617026 hasRelatedWork W2165045641 @default.
• W2066617026 hasRelatedWork W2175737517 @default.
• W2066617026 hasRelatedWork W2288927333 @default.
• W2066617026 hasRelatedWork W2350738313 @default.
• W2066617026 hasRelatedWork W4255694338 @default.
• W2066617026 hasVolume "54" @default.
• W2066617026 isParatext "false" @default.
• W2066617026 isRetracted "false" @default.
• W2066617026 magId "2066617026" @default.
• W2066617026 workType "article" @default.