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• W2066646064 startingPage "4453" @default.
• W2066646064 abstract "The hexanucleoside pentaphosphate d(m5CpGpm5CpGpm5CpG) has been studied in solution by ultra-violet absorption, circular dichroism and 31P nuclear magnetic resonance under various experimental conditions. In 0.2 M NaClO4 at low temperature, an hexamer duplex is formed which has a B or B-like conformation. As the salt concentration is increased, a transition from a B-form to the Z-form occurs and is complete in 3 M NaClO4. In 3 M NaClO4, the behavior of the Z double helix is complex as a function of temperature. The variation of the circular dichroism at 295 nm is biphasic. A first transition occurs over a large range of temperature and corresponds to a conformational change due to a non-cooperative intramolecular process. Ultra-violet absorption and 31P nuclear magnetic resonance show that the new conformation arising from a distortion of the backbone is not similar to that observed in low salt conditions (B-form). At high hexanucleotide concentration, aggregates are formed. The second transition is cooperative and corresponds to the melting of a double stranded helix into single strands." @default.
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• W2066646064 date "1983-01-01" @default.
• W2066646064 modified "2023-09-25" @default.
• W2066646064 title "Spectroscopic studies of (m⁵dC-dG)₃: thermal stability of B- and Z-forms" @default.
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• W2066646064 doi "https://doi.org/10.1093/nar/11.13.4453" @default.
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