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- W2066706024 abstract "Abstract The interaction between aminocarb and calf thymus DNA in physiological buffer (pH 7.4) was investigated by UV–vis spectrophotometry, fluorescence spectroscopy, viscosity measurements and DNA melting techniques. The absorption spectra of aminocarb with DNA showed a slight blue shift and hypochromic effect. Using ethidium bromide (EB) as a fluorescence probe, fluorescence quenching of the emission peak was seen in the DNA–EB system when aminocarb was added. The fluorescence polarization was gradually increased with increasing amounts of DNA. The value of melting temperature of DNA increased in the presence of aminocarb. Moreover, the relative viscosity of DNA increased with the addition of aminocarb. All the evidences indicated that the binding mode of aminocarb with DNA was an intercalative binding. The binding constants of aminocarb with DNA were determined. The calculated thermodynamic parameters suggested that the binding of aminocarb to DNA was driven mainly by hydrogen bond and van der Waals." @default.
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- W2066706024 date "2010-10-01" @default.
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- W2066706024 title "Studies on the interaction of aminocarb with calf thymus DNA by spectroscopic methods" @default.
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- W2066706024 doi "https://doi.org/10.1016/j.pestbp.2010.06.008" @default.
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