FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066741161> ?p ?o ?g. }

• W2066741161 endingPage "348" @default.
• W2066741161 startingPage "334" @default.
• W2066741161 abstract "We have characterized two components of DNA isolated from mouse L-M cell nuclei. These components, designated as HMW (high molecular weight) and VHMW (very high molecular weight) DNA, were characterized by rate zonal sedimentation, agarose gel electrophoresis, and for protein content. Our electron micrographs revealed that HMW-DNA contained mainly linear molecules with few single rosette structures, while the VHMW-DNA was enriched in rosettes, many of which were significantly larger and linked together in multimeric structural forms. The VHMW-DNA component was also enriched for residual protein, which we believe represents the core of the rosette. The characteristics of this residual protein are consistent with reported findings of the most tightly bound proteins. The rosette conformation does not appear to be an artifact of microscopy or of an aggregate nature for several reasons: (i) rosettes are preferentially found in the VHMW-DNA component; (ii) further manipulation or purification of the DNA disrupts the rosette structure and produces linear fragments; (iii) the amount of proteinaceous material at the core of the rosette is diminished when the DNA is further purified; and (iv) treatment of intact nuclei with a novel bisamine reagent putatively crosslinks DNA in vivo and minimizes the disruption of rosettes by shear. We believe this separation of chromatin is critical to establish the architectural forms of euchromatin and heterochromatin of interphase DNA in the eucaryotic system. Once established, fractionated chromatin can be used to identify specifically expressed or repressed genes with linear form DNA and rosette form DNA. We discuss rosettes as derivatives of chromosomal domains that retain structural features because of residual peptide elements." @default.
• W2066741161 created "2016-06-24" @default.
• W2066741161 creator A5015206540 @default.
• W2066741161 creator A5025265943 @default.
• W2066741161 creator A5038543673 @default.
• W2066741161 creator A5054908786 @default.
• W2066741161 creator A5061886250 @default.
• W2066741161 date "1988-06-01" @default.
• W2066741161 modified "2023-10-16" @default.
• W2066741161 title "Identification of a rosette-enriched chromatin fraction from mouse fibroblast nuclei" @default.
• W2066741161 cites W1131804799 @default.
• W2066741161 cites W1449454959 @default.
• W2066741161 cites W1487210188 @default.
• W2066741161 cites W1531014909 @default.
• W2066741161 cites W1579594364 @default.
• W2066741161 cites W1606423922 @default.
• W2066741161 cites W1625660792 @default.
• W2066741161 cites W1890236332 @default.
• W2066741161 cites W1964376188 @default.
• W2066741161 cites W1965334884 @default.
• W2066741161 cites W1968386466 @default.
• W2066741161 cites W1968474740 @default.
• W2066741161 cites W1982368633 @default.
• W2066741161 cites W1984489982 @default.
• W2066741161 cites W1984494784 @default.
• W2066741161 cites W1998265298 @default.
• W2066741161 cites W2005389848 @default.
• W2066741161 cites W2006216882 @default.
• W2066741161 cites W2013571070 @default.
• W2066741161 cites W2017422614 @default.
• W2066741161 cites W2018491753 @default.
• W2066741161 cites W2023412006 @default.
• W2066741161 cites W2034277598 @default.
• W2066741161 cites W2036648250 @default.
• W2066741161 cites W2038447185 @default.
• W2066741161 cites W2040119409 @default.
• W2066741161 cites W2048799557 @default.
• W2066741161 cites W2050247067 @default.
• W2066741161 cites W2051616170 @default.
• W2066741161 cites W2057723914 @default.
• W2066741161 cites W2059752413 @default.
• W2066741161 cites W2060832808 @default.
• W2066741161 cites W2062461574 @default.
• W2066741161 cites W2069958185 @default.
• W2066741161 cites W2071478793 @default.
• W2066741161 cites W2075076728 @default.
• W2066741161 cites W2082287188 @default.
• W2066741161 cites W2100837269 @default.
• W2066741161 cites W2105054597 @default.
• W2066741161 cites W2116989970 @default.
• W2066741161 cites W2119460480 @default.
• W2066741161 cites W2142617031 @default.
• W2066741161 cites W2174983906 @default.
• W2066741161 cites W2460191387 @default.
• W2066741161 cites W38643767 @default.
• W2066741161 cites W4231909112 @default.
• W2066741161 cites W4250052852 @default.
• W2066741161 doi "https://doi.org/10.1016/0003-9861(88)90644-3" @default.
• W2066741161 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3377507" @default.
• W2066741161 hasPublicationYear "1988" @default.
• W2066741161 type Work @default.
• W2066741161 sameAs 2066741161 @default.
• W2066741161 citedByCount "10" @default.
• W2066741161 crossrefType "journal-article" @default.
• W2066741161 hasAuthorship W2066741161A5015206540 @default.
• W2066741161 hasAuthorship W2066741161A5025265943 @default.
• W2066741161 hasAuthorship W2066741161A5038543673 @default.
• W2066741161 hasAuthorship W2066741161A5054908786 @default.
• W2066741161 hasAuthorship W2066741161A5061886250 @default.
• W2066741161 hasConcept C100924687 @default.
• W2066741161 hasConcept C103435993 @default.
• W2066741161 hasConcept C12554922 @default.
• W2066741161 hasConcept C1388644 @default.
• W2066741161 hasConcept C153911025 @default.
• W2066741161 hasConcept C181199279 @default.
• W2066741161 hasConcept C203014093 @default.
• W2066741161 hasConcept C2778476535 @default.
• W2066741161 hasConcept C2781414804 @default.
• W2066741161 hasConcept C29452124 @default.
• W2066741161 hasConcept C552990157 @default.
• W2066741161 hasConcept C55493867 @default.
• W2066741161 hasConcept C83640560 @default.
• W2066741161 hasConcept C86803240 @default.
• W2066741161 hasConcept C95444343 @default.
• W2066741161 hasConceptScore W2066741161C100924687 @default.
• W2066741161 hasConceptScore W2066741161C103435993 @default.
• W2066741161 hasConceptScore W2066741161C12554922 @default.
• W2066741161 hasConceptScore W2066741161C1388644 @default.
• W2066741161 hasConceptScore W2066741161C153911025 @default.
• W2066741161 hasConceptScore W2066741161C181199279 @default.
• W2066741161 hasConceptScore W2066741161C203014093 @default.
• W2066741161 hasConceptScore W2066741161C2778476535 @default.
• W2066741161 hasConceptScore W2066741161C2781414804 @default.
• W2066741161 hasConceptScore W2066741161C29452124 @default.
• W2066741161 hasConceptScore W2066741161C552990157 @default.
• W2066741161 hasConceptScore W2066741161C55493867 @default.
• W2066741161 hasConceptScore W2066741161C83640560 @default.
• W2066741161 hasConceptScore W2066741161C86803240 @default.

• next