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- W2066825562 abstract "During exponential growth, Erwinia chrysanthemi (EC16) exports 99% of the protease (PRT) into the growth medium. By screening an EC16 genomic library in Escherichia coli HB101, several Prt+ clones were identified. A 16-kb EcoRI fragment, carrying the prt gene, was subcloned into pBR322 (pAKC326). E. coli HB101[pAKC326] cells exported PRT into the growth medium during exponential growth. PRT export was not accompanied by periplasmic leakage. E. coli HB101 carrying EC16 prt and pel genes (encoding pectate lyase) exported PRT but retained PEL in the periplasm. These findings indicate the occurrence of a PRT-specific export system in EC16, which is also functional in an E. coli strain carrying the prt+ DNA segment." @default.
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- W2066825562 date "1986-05-01" @default.
- W2066825562 modified "2023-09-23" @default.
- W2066825562 title "Export ofErwinia chrysanthemi(EC16) protease byEscherichia coli" @default.
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- W2066825562 doi "https://doi.org/10.1111/j.1574-6968.1986.tb01434.x" @default.
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