Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066829899> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2066829899 endingPage "S69" @default.
- W2066829899 startingPage "S69" @default.
- W2066829899 abstract "Preeclampsia (PE) is one of the leading causes of maternal and perinatal morbidity and mortality worldwide. Abnormal expression of microRNAs (miRNAs) occurs in several pregnancy diseases including PE. Placental trophoblast cells express a specific set of miRNAs which changes during pregnancy. These miRNAs can be released within extracellular vesicles (EVs) and mediate intercellular communication. miR-141 is a pregnancy-related miRNA which is expressed by trophoblast cells at increased levels in maternal plasma in the third trimester. We hypothesize that miR-141 is abnormally expressed in PE placentae, controls trophoblast, and immune cell functions and is involved in the intercellular communication between fetal trophoblast and maternal immune cells. Expression of miR-141 was analyzed by quantitative real-time PCR (qPCR) in normal and preeclamptic placentae and in 2 different trophoblastic cell lines, JEG-3 and HTR-8/SVneo. Changes in JEG-3 and HTR-8/SVneo cell proliferation and invasion were investigated after miR-141 inhibition and overexpression by MTS-, BrdU-, and Matrigel assays. EVs from miR-141 transfected cells were isolated from supernatants and characterized by NanoSight analysis and qPCR. Proliferation of Jurkat T cells and invasion of HTR-8/SVneo cells were investigated after treatment with EVs containing different miR-141 levels. miR-141 expression was higher in placentae from PE patients compared with those from normal pregnancies. miR-141 inhibition in trophoblastic cells resulted in decreased cell viability and reduced invasion capability. After transfection with miR-141-mimic, trophoblastic cells secreted EVs with increased miR-141 content. These vesicles did not exert effects on trophoblastic cell invasion but reduced Jurkat T cell proliferation. In conclusion, miR-141 regulates major functions of trophoblastic and immune cells. Trophoblast cells release EVs whose miRNA content can be modified by transfection of origin cells. Furthermore, elevated levels of miR-141 can be transferred from trophoblast to immune cells by release and internalization of EVs suggesting their role in the immune regulation of normal and pathologic pregnancies." @default.
- W2066829899 created "2016-06-24" @default.
- W2066829899 creator A5000574565 @default.
- W2066829899 creator A5021043752 @default.
- W2066829899 creator A5034115421 @default.
- W2066829899 creator A5044544312 @default.
- W2066829899 creator A5055581618 @default.
- W2066829899 creator A5070178403 @default.
- W2066829899 creator A5071914625 @default.
- W2066829899 creator A5076010911 @default.
- W2066829899 date "2010-10-01" @default.
- W2066829899 modified "2023-09-26" @default.
- W2066829899 title "P99 Clinical complications in patients with near miss criteria admitted at an obstetric intensive care unit in Pernambuco, Brazil: preliminary result" @default.
- W2066829899 doi "https://doi.org/10.1016/s2210-7789(10)60265-6" @default.
- W2066829899 hasPublicationYear "2010" @default.
- W2066829899 type Work @default.
- W2066829899 sameAs 2066829899 @default.
- W2066829899 citedByCount "0" @default.
- W2066829899 crossrefType "journal-article" @default.
- W2066829899 hasAuthorship W2066829899A5000574565 @default.
- W2066829899 hasAuthorship W2066829899A5021043752 @default.
- W2066829899 hasAuthorship W2066829899A5034115421 @default.
- W2066829899 hasAuthorship W2066829899A5044544312 @default.
- W2066829899 hasAuthorship W2066829899A5055581618 @default.
- W2066829899 hasAuthorship W2066829899A5070178403 @default.
- W2066829899 hasAuthorship W2066829899A5071914625 @default.
- W2066829899 hasAuthorship W2066829899A5076010911 @default.
- W2066829899 hasConcept C104317684 @default.
- W2066829899 hasConcept C145059251 @default.
- W2066829899 hasConcept C16685009 @default.
- W2066829899 hasConcept C172680121 @default.
- W2066829899 hasConcept C203014093 @default.
- W2066829899 hasConcept C2776953305 @default.
- W2066829899 hasConcept C2777218350 @default.
- W2066829899 hasConcept C2777495146 @default.
- W2066829899 hasConcept C2778608917 @default.
- W2066829899 hasConcept C2779234561 @default.
- W2066829899 hasConcept C502942594 @default.
- W2066829899 hasConcept C54009773 @default.
- W2066829899 hasConcept C54355233 @default.
- W2066829899 hasConcept C71924100 @default.
- W2066829899 hasConcept C81885089 @default.
- W2066829899 hasConcept C86803240 @default.
- W2066829899 hasConcept C8891405 @default.
- W2066829899 hasConcept C95444343 @default.
- W2066829899 hasConceptScore W2066829899C104317684 @default.
- W2066829899 hasConceptScore W2066829899C145059251 @default.
- W2066829899 hasConceptScore W2066829899C16685009 @default.
- W2066829899 hasConceptScore W2066829899C172680121 @default.
- W2066829899 hasConceptScore W2066829899C203014093 @default.
- W2066829899 hasConceptScore W2066829899C2776953305 @default.
- W2066829899 hasConceptScore W2066829899C2777218350 @default.
- W2066829899 hasConceptScore W2066829899C2777495146 @default.
- W2066829899 hasConceptScore W2066829899C2778608917 @default.
- W2066829899 hasConceptScore W2066829899C2779234561 @default.
- W2066829899 hasConceptScore W2066829899C502942594 @default.
- W2066829899 hasConceptScore W2066829899C54009773 @default.
- W2066829899 hasConceptScore W2066829899C54355233 @default.
- W2066829899 hasConceptScore W2066829899C71924100 @default.
- W2066829899 hasConceptScore W2066829899C81885089 @default.
- W2066829899 hasConceptScore W2066829899C86803240 @default.
- W2066829899 hasConceptScore W2066829899C8891405 @default.
- W2066829899 hasConceptScore W2066829899C95444343 @default.
- W2066829899 hasLocation W20668298991 @default.
- W2066829899 hasOpenAccess W2066829899 @default.
- W2066829899 hasPrimaryLocation W20668298991 @default.
- W2066829899 hasRelatedWork W1999786268 @default.
- W2066829899 hasRelatedWork W2119084006 @default.
- W2066829899 hasRelatedWork W2987602267 @default.
- W2066829899 hasRelatedWork W3030512222 @default.
- W2066829899 hasRelatedWork W3030934367 @default.
- W2066829899 hasRelatedWork W3031293994 @default.
- W2066829899 hasRelatedWork W3134489679 @default.
- W2066829899 hasRelatedWork W3169010924 @default.
- W2066829899 hasRelatedWork W3196597502 @default.
- W2066829899 hasRelatedWork W4226453156 @default.
- W2066829899 hasVolume "1" @default.
- W2066829899 isParatext "false" @default.
- W2066829899 isRetracted "false" @default.
- W2066829899 magId "2066829899" @default.
- W2066829899 workType "article" @default.