FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066883938> ?p ?o ?g. }

• W2066883938 endingPage "2450" @default.
• W2066883938 startingPage "2438" @default.
• W2066883938 abstract "Cross-presentation (CP) is important for priming T cell responses to many viral, bacterial, and tumor antigens. Here, we designed two Ii mutants, based on evidence that the invariant chain (Ii, also named CD74) binds newly synthesized MHC class I molecules with the class II-associated invariant chain peptide (CLIP) region of Ii, which occupies the peptide-binding groove. Specifically, we designed (1) Ii-O257, which is a CLIP-substituted Ii chimer, in which OVA257-264 (SIINFEKL) was substituted for CLIP, and (2) Ii-, also named CT257, which is a C-terminal truncated form of Ii-O257 that contains the N-terminal flanking region of Ii. We immunized C57BL/6 mice with these recombinant proteins. Real-time PCR detected that mice immunized with either Ii-O257 or Ii-CT257 recombinant proteins exhibited increased IFN-γ mRNA expression (approximately 11-fold and 13-fold, respectively) and increased IL-2 mRNA expression (approximately 9-fold and 11-fold, respectively), compared to mice immunized with the OVA257-264 peptide. In vivo cytokine analysis showed that recombinant Ii proteins were highly efficient at activating T cells. Confocal microscopy and co-immunoprecipitation showed that the 2 Ii-OVA257-264 chimers are associated intracellularly with H-2K(b) molecules. Thus, Ii-CT257 (amino acids 1-89) binds stably to MHC class I with high affinity, indicating that it is a minimal functional fragment of the Ii immune vector. In conclusion, the N-terminal functional region of the Ii fusion protein containing CTL epitopes might prove to be useful for developing peptide or DNA vaccines that use CP as the main mechanism for CD8(+) T cell stimulation." @default.
• W2066883938 created "2016-06-24" @default.
• W2066883938 creator A5013566286 @default.
• W2066883938 creator A5025243010 @default.
• W2066883938 creator A5032319118 @default.
• W2066883938 creator A5054367424 @default.
• W2066883938 creator A5067981828 @default.
• W2066883938 creator A5080065977 @default.
• W2066883938 date "2014-01-01" @default.
• W2066883938 modified "2023-10-16" @default.
• W2066883938 title "N-terminal functional region of the invariant chain efficiently targets the binding of a CTL epitope to MHC class I molecules during cross-presentation" @default.
• W2066883938 cites W147604088 @default.
• W2066883938 cites W1497968041 @default.
• W2066883938 cites W1547134586 @default.
• W2066883938 cites W1551798452 @default.
• W2066883938 cites W1663973802 @default.
• W2066883938 cites W1965546174 @default.
• W2066883938 cites W1971645594 @default.
• W2066883938 cites W1982944034 @default.
• W2066883938 cites W2009378348 @default.
• W2066883938 cites W2011283910 @default.
• W2066883938 cites W2012324563 @default.
• W2066883938 cites W2016255773 @default.
• W2066883938 cites W2018925832 @default.
• W2066883938 cites W2030348506 @default.
• W2066883938 cites W2034832824 @default.
• W2066883938 cites W2035780364 @default.
• W2066883938 cites W2058473193 @default.
• W2066883938 cites W2066844508 @default.
• W2066883938 cites W2068290887 @default.
• W2066883938 cites W2095843724 @default.
• W2066883938 cites W2096273252 @default.
• W2066883938 cites W2107140931 @default.
• W2066883938 cites W2119045499 @default.
• W2066883938 cites W2126542048 @default.
• W2066883938 cites W2130863508 @default.
• W2066883938 cites W2163690467 @default.
• W2066883938 cites W2168484579 @default.
• W2066883938 doi "https://doi.org/10.4238/2014.april.3.16" @default.
• W2066883938 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24781998" @default.
• W2066883938 hasPublicationYear "2014" @default.
• W2066883938 type Work @default.
• W2066883938 sameAs 2066883938 @default.
• W2066883938 citedByCount "4" @default.
• W2066883938 countsByYear W20668839382014 @default.
• W2066883938 countsByYear W20668839382017 @default.
• W2066883938 countsByYear W20668839382020 @default.
• W2066883938 countsByYear W20668839382021 @default.
• W2066883938 crossrefType "journal-article" @default.
• W2066883938 hasAuthorship W2066883938A5013566286 @default.
• W2066883938 hasAuthorship W2066883938A5025243010 @default.
• W2066883938 hasAuthorship W2066883938A5032319118 @default.
• W2066883938 hasAuthorship W2066883938A5054367424 @default.
• W2066883938 hasAuthorship W2066883938A5067981828 @default.
• W2066883938 hasAuthorship W2066883938A5080065977 @default.
• W2066883938 hasBestOaLocation W20668839381 @default.
• W2066883938 hasConcept C104317684 @default.
• W2066883938 hasConcept C119997001 @default.
• W2066883938 hasConcept C123894998 @default.
• W2066883938 hasConcept C147483822 @default.
• W2066883938 hasConcept C147969180 @default.
• W2066883938 hasConcept C153911025 @default.
• W2066883938 hasConcept C167672396 @default.
• W2066883938 hasConcept C170627219 @default.
• W2066883938 hasConcept C185592680 @default.
• W2066883938 hasConcept C195616568 @default.
• W2066883938 hasConcept C203014093 @default.
• W2066883938 hasConcept C207936829 @default.
• W2066883938 hasConcept C2776090121 @default.
• W2066883938 hasConcept C2778814158 @default.
• W2066883938 hasConcept C2779281246 @default.
• W2066883938 hasConcept C40767141 @default.
• W2066883938 hasConcept C55493867 @default.
• W2066883938 hasConcept C71829478 @default.
• W2066883938 hasConcept C83464605 @default.
• W2066883938 hasConcept C86803240 @default.
• W2066883938 hasConcept C8891405 @default.
• W2066883938 hasConceptScore W2066883938C104317684 @default.
• W2066883938 hasConceptScore W2066883938C119997001 @default.
• W2066883938 hasConceptScore W2066883938C123894998 @default.
• W2066883938 hasConceptScore W2066883938C147483822 @default.
• W2066883938 hasConceptScore W2066883938C147969180 @default.
• W2066883938 hasConceptScore W2066883938C153911025 @default.
• W2066883938 hasConceptScore W2066883938C167672396 @default.
• W2066883938 hasConceptScore W2066883938C170627219 @default.
• W2066883938 hasConceptScore W2066883938C185592680 @default.
• W2066883938 hasConceptScore W2066883938C195616568 @default.
• W2066883938 hasConceptScore W2066883938C203014093 @default.
• W2066883938 hasConceptScore W2066883938C207936829 @default.
• W2066883938 hasConceptScore W2066883938C2776090121 @default.
• W2066883938 hasConceptScore W2066883938C2778814158 @default.
• W2066883938 hasConceptScore W2066883938C2779281246 @default.
• W2066883938 hasConceptScore W2066883938C40767141 @default.
• W2066883938 hasConceptScore W2066883938C55493867 @default.
• W2066883938 hasConceptScore W2066883938C71829478 @default.
• W2066883938 hasConceptScore W2066883938C83464605 @default.
• W2066883938 hasConceptScore W2066883938C86803240 @default.
• W2066883938 hasConceptScore W2066883938C8891405 @default.

• next