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- W2066894984 abstract "The attachment and growth of an established cell line derived from mouse fibroblasts on collagen, chemically modified collagen, and collagen composite surfaces were compared. Tissue culture polystyrene dishes provided a suitable control. The substrates included native bovine dermal collagen, succinylated, acetylated and methylated collagen, and a series of composite materials formed from collagen and the glycosaminoglycans hyaluronic acid, chondroitin 4-sulphate and chondroitin 6-sulphate and the glycoprotein fibronectin. Attachment and growth of cells on each of these substrates were assessed by visual inspection under optical microscopy, by detachment of the cells using trypsinization and subsequent counting in a Coulter counter; and by 3H-thymidine incorporation studies. A very good correlation between the results was obtained by the three methods employed which showed that collagen, in comparison to polystyrene, is a relatively poor substrate for cellular attachment, growth and proliferation, but it may be improved by chemical modification and by incorporation of either fibronectin, chondroitin sulphate (5 and 10%), or low levels (less than 5%) of hyaluronic acid into the collagen matrix. Concentrations in excess of 5% hyaluronic acid into the collagen matrix, however, appeared to inhibit cellular attachment and growth and such materials provided a poorer substrate than native collagen." @default.
- W2066894984 created "2016-06-24" @default.
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- W2066894984 date "1990-04-01" @default.
- W2066894984 modified "2023-09-26" @default.
- W2066894984 title "The attachment and growth of an established cell line on collagen, chemically modified collagen, and collagen composite surfaces" @default.
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- W2066894984 doi "https://doi.org/10.1016/0142-9612(90)90149-k" @default.
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