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- W2066922920 abstract "Abstract To perform enzyme-catalysed bromination reactions, the enzyme myeloperoxidase (MPO; EC 1.11.1.7) from human polymorphonuclear leucocytes was immobilized onto controlled pore glass (CPG, pore size 50 nm). Different coupling techniques were screened and enzyme-loaded glass beads subjected to storage stability tests. The best results are obtained with CPG derivatized with cyanogen bromide. Different immobilization conditions were examined. Optimized enzyme immobilization was performed as follows: 0.05 U MPO, dissolved in 250 μl 0.3 m phosphate buffer pH 7.0, containing 0.3 mg ml −1 bovine serum albumin (BSA) was added to 75 mg CNBr-derivatized glass beads. The reaction mixture was kept at 0°C for 2 h, and every 10 min a shaking period of a few seconds was applied." @default.
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- W2066922920 date "1988-11-01" @default.
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- W2066922920 title "Immobilization of human polymorphonuclear leucocyte myeloperoxidase onto controlled pore glass derivatives" @default.
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- W2066922920 doi "https://doi.org/10.1016/0141-0229(88)90056-7" @default.
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