FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2066964328> ?p ?o ?g. }

• W2066964328 abstract "Glutamate synthase (GltS) is a complex iron-sulfur flavoprotein that catalyzes the reductive transfer of L-glutamine amide group to the C2 carbon of 2-oxoglutarate yielding two molecules of L-glutamate. Molecular dynamics calculations in explicit solvent were carried out to gain insight into the conformational flexibility of GltS and into the role played by the enzyme substrates in regulating the catalytic cycle. We have modelled the free (unliganded) form of Azospirillum brasilense GltS alpha subunit and the structure of the reduced enzyme in complex with the L-glutamine and 2-oxoglutarate substrates starting from the crystallographically determined coordinates of the GltS alpha subunit in complex with L-methionine sulphone and 2-oxoglutarate. The present 4-ns molecular dynamics calculations reveal that the GltS glutaminase site may exist in a catalytically inactive conformation unable to bind glutamine, and in a catalytically competent conformation, which is stabilized by the glutamine substrate. Substrates binding also induce (1) closure of the loop formed by residues 263-271 with partial shielding of the glutaminase site from solvent, and (2) widening of the ammonia tunnel entrance at the glutaminase end to allow for ammonia diffusion toward the synthase site. The Q-loop of glutamate synthase, which acts as an active site lid in other amidotransferases, seems to maintain an open conformation. Finally, binding of L-methionine sulfone, a glutamine analog that mimics the tetrahedral transient species occurring during its hydrolysis, causes a coordinated rigid-body motion of segments of the glutaminase domain that results in the inactive conformation observed in the crystal structure of GltS alpha subunit." @default.
• W2066964328 created "2016-06-24" @default.
• W2066964328 creator A5008633893 @default.
• W2066964328 creator A5046225954 @default.
• W2066964328 creator A5058858104 @default.
• W2066964328 creator A5060901464 @default.
• W2066964328 creator A5068361493 @default.
• W2066964328 creator A5082342886 @default.
• W2066964328 creator A5090817979 @default.
• W2066964328 date "2008-12-29" @default.
• W2066964328 modified "2023-10-01" @default.
• W2066964328 title "Molecular dynamics simulation of the interaction between the complex iron-sulfur flavoprotein glutamate synthase and its substrates" @default.
• W2066964328 cites W1491824558 @default.
• W2066964328 cites W1771464347 @default.
• W2066964328 cites W1965270719 @default.
• W2066964328 cites W1970289489 @default.
• W2066964328 cites W1975172012 @default.
• W2066964328 cites W1982345335 @default.
• W2066964328 cites W2009485382 @default.
• W2066964328 cites W2026226194 @default.
• W2066964328 cites W2026258231 @default.
• W2066964328 cites W2027957346 @default.
• W2066964328 cites W2040503203 @default.
• W2066964328 cites W2043329863 @default.
• W2066964328 cites W2049115309 @default.
• W2066964328 cites W2050107237 @default.
• W2066964328 cites W2054329140 @default.
• W2066964328 cites W2073071638 @default.
• W2066964328 cites W2079120177 @default.
• W2066964328 cites W2080766922 @default.
• W2066964328 cites W2081604308 @default.
• W2066964328 cites W2085818775 @default.
• W2066964328 cites W2088969806 @default.
• W2066964328 cites W2090736922 @default.
• W2066964328 cites W2091563009 @default.
• W2066964328 cites W2117332309 @default.
• W2066964328 cites W2123768693 @default.
• W2066964328 cites W2127272780 @default.
• W2066964328 cites W2132589721 @default.
• W2066964328 cites W2141519313 @default.
• W2066964328 cites W2145380863 @default.
• W2066964328 cites W2146228523 @default.
• W2066964328 cites W2150092099 @default.
• W2066964328 cites W2980675798 @default.
• W2066964328 cites W3022141464 @default.
• W2066964328 doi "https://doi.org/10.1110/ps.04863104" @default.
• W2066964328 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2286594" @default.
• W2066964328 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15498940" @default.
• W2066964328 hasPublicationYear "2008" @default.
• W2066964328 type Work @default.
• W2066964328 sameAs 2066964328 @default.
• W2066964328 citedByCount "9" @default.
• W2066964328 countsByYear W20669643282012 @default.
• W2066964328 countsByYear W20669643282013 @default.
• W2066964328 countsByYear W20669643282015 @default.
• W2066964328 crossrefType "journal-article" @default.
• W2066964328 hasAuthorship W2066964328A5008633893 @default.
• W2066964328 hasAuthorship W2066964328A5046225954 @default.
• W2066964328 hasAuthorship W2066964328A5058858104 @default.
• W2066964328 hasAuthorship W2066964328A5060901464 @default.
• W2066964328 hasAuthorship W2066964328A5068361493 @default.
• W2066964328 hasAuthorship W2066964328A5082342886 @default.
• W2066964328 hasAuthorship W2066964328A5090817979 @default.
• W2066964328 hasBestOaLocation W20669643282 @default.
• W2066964328 hasConcept C109549099 @default.
• W2066964328 hasConcept C112243037 @default.
• W2066964328 hasConcept C181199279 @default.
• W2066964328 hasConcept C185592680 @default.
• W2066964328 hasConcept C191771488 @default.
• W2066964328 hasConcept C2779289688 @default.
• W2066964328 hasConcept C2779349466 @default.
• W2066964328 hasConcept C41183919 @default.
• W2066964328 hasConcept C515207424 @default.
• W2066964328 hasConcept C55493867 @default.
• W2066964328 hasConcept C63338738 @default.
• W2066964328 hasConcept C71240020 @default.
• W2066964328 hasConcept C71995715 @default.
• W2066964328 hasConcept C75972863 @default.
• W2066964328 hasConceptScore W2066964328C109549099 @default.
• W2066964328 hasConceptScore W2066964328C112243037 @default.
• W2066964328 hasConceptScore W2066964328C181199279 @default.
• W2066964328 hasConceptScore W2066964328C185592680 @default.
• W2066964328 hasConceptScore W2066964328C191771488 @default.
• W2066964328 hasConceptScore W2066964328C2779289688 @default.
• W2066964328 hasConceptScore W2066964328C2779349466 @default.
• W2066964328 hasConceptScore W2066964328C41183919 @default.
• W2066964328 hasConceptScore W2066964328C515207424 @default.
• W2066964328 hasConceptScore W2066964328C55493867 @default.
• W2066964328 hasConceptScore W2066964328C63338738 @default.
• W2066964328 hasConceptScore W2066964328C71240020 @default.
• W2066964328 hasConceptScore W2066964328C71995715 @default.
• W2066964328 hasConceptScore W2066964328C75972863 @default.
• W2066964328 hasLocation W20669643281 @default.
• W2066964328 hasLocation W20669643282 @default.
• W2066964328 hasLocation W20669643283 @default.
• W2066964328 hasLocation W20669643284 @default.
• W2066964328 hasOpenAccess W2066964328 @default.
• W2066964328 hasPrimaryLocation W20669643281 @default.
• W2066964328 hasRelatedWork W1974784744 @default.
• W2066964328 hasRelatedWork W1981116229 @default.

• next