FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W206702936> ?p ?o ?g. }

• W206702936 abstract "Lymphoblastoid cell lines (LCLs) are created by culturing lymphocytes from the peripheral blood and adding Epstein-Barr virus (EBV), a ubiquitous human herpesvirus which infects, activates, and transforms B cells. These cell lines are used for genotyping, as targets for cytotoxic cells, and as models for EBV immortalization of B cells, particularly post-transplant lymphoproliferative disease (PTLD) in which EBV-immortalized cells proliferate in the absence of a cytotoxic T-cell response. Studies have shown more diversity in LCLs than would be expected from cell lines that are often treated as interchangeable. It is not known how their diversity in factors like morphology, growth factor production, or cellular gene expression influences the EBV life cycle. In this study I investigated connections between LCLs' cellular and viral phenotypes, categorizing them as either low in EBV copy number or fluctuating within a high range. As measured by lytic EBV replication and viral gene expression, LCLs showed high or low lytic permissivity, with permissivity defined as the likelihood that a cell will switch from stable latent infection into the lytic EBV life cycle. Permissivity was not affected by blocking the late events of the lytic cycle. I used flow cytometry to characterize 19 aspects of LCL surface phenotype, but found little association with lytic permissivity. Microarrays and PCR were used to identify genes expressed at higher levels in non-permissive LCLs, including transcription factors that maintain B cell lineage. Unfolded protein response (UPR) genes and the UPR protein Grp94 were expressed at higher levels in permissive LCLs. A drug was used to investigate effects of the UPR on permissive and non-permissive LCLs that had been maintained for short or long periods of time. The UPR enhanced permissivity, causing more cells to enter the lytic cycle, but this did not lead to lytic replication. This study enhances our knowledge about EBV life cycles by giving us new information about host factors that contribute to the lytic switch. This data about LCL diversity has public health relevance to the diversity of PTLD cases, since identifying risk factors for PTLD is a significant part of care for EBV-positive transplant recipients." @default.
• W206702936 created "2016-06-24" @default.
• W206702936 creator A5025424183 @default.
• W206702936 date "2010-09-29" @default.
• W206702936 modified "2023-09-27" @default.
• W206702936 title "CELLULAR PATHWAYS LEADING TO PATTERNS OF LYTIC EPSTEIN-BARR VIRUS REACTIVATION IN IMMORTALIZED B CELL LINES" @default.
• W206702936 cites W118886063 @default.
• W206702936 cites W142221236 @default.
• W206702936 cites W1521497390 @default.
• W206702936 cites W1526716359 @default.
• W206702936 cites W1531490664 @default.
• W206702936 cites W1531680230 @default.
• W206702936 cites W154512713 @default.
• W206702936 cites W1547907664 @default.
• W206702936 cites W1555720870 @default.
• W206702936 cites W1561042888 @default.
• W206702936 cites W156627268 @default.
• W206702936 cites W1568687854 @default.
• W206702936 cites W1573924111 @default.
• W206702936 cites W1598586133 @default.
• W206702936 cites W1609762454 @default.
• W206702936 cites W1799806109 @default.
• W206702936 cites W1848439552 @default.
• W206702936 cites W1854068525 @default.
• W206702936 cites W1886729928 @default.
• W206702936 cites W189186486 @default.
• W206702936 cites W1912540205 @default.
• W206702936 cites W191615340 @default.
• W206702936 cites W1917600494 @default.
• W206702936 cites W1938270372 @default.
• W206702936 cites W1944597139 @default.
• W206702936 cites W1951147112 @default.
• W206702936 cites W1964027703 @default.
• W206702936 cites W1966831463 @default.
• W206702936 cites W1967413974 @default.
• W206702936 cites W1967874493 @default.
• W206702936 cites W1968262050 @default.
• W206702936 cites W1969118436 @default.
• W206702936 cites W1969322596 @default.
• W206702936 cites W1970090817 @default.
• W206702936 cites W1971542939 @default.
• W206702936 cites W1972167991 @default.
• W206702936 cites W1972336872 @default.
• W206702936 cites W1974010997 @default.
• W206702936 cites W1974102799 @default.
• W206702936 cites W1975308879 @default.
• W206702936 cites W1975562645 @default.
• W206702936 cites W1977148500 @default.
• W206702936 cites W1977912281 @default.
• W206702936 cites W1979401674 @default.
• W206702936 cites W1979976650 @default.
• W206702936 cites W1981421729 @default.
• W206702936 cites W1982133573 @default.
• W206702936 cites W1988224876 @default.
• W206702936 cites W1988472957 @default.
• W206702936 cites W1989444544 @default.
• W206702936 cites W1990490819 @default.
• W206702936 cites W1991253015 @default.
• W206702936 cites W1992918232 @default.
• W206702936 cites W1992921284 @default.
• W206702936 cites W1993542864 @default.
• W206702936 cites W1993688639 @default.
• W206702936 cites W1993839005 @default.
• W206702936 cites W1995653774 @default.
• W206702936 cites W1996212620 @default.
• W206702936 cites W1997010494 @default.
• W206702936 cites W1997145514 @default.
• W206702936 cites W1997827395 @default.
• W206702936 cites W1997904153 @default.
• W206702936 cites W1998914928 @default.
• W206702936 cites W1999695678 @default.
• W206702936 cites W2000584697 @default.
• W206702936 cites W2000684462 @default.
• W206702936 cites W2001158900 @default.
• W206702936 cites W2001224935 @default.
• W206702936 cites W2001699583 @default.
• W206702936 cites W2002223549 @default.
• W206702936 cites W2002765315 @default.
• W206702936 cites W2005049291 @default.
• W206702936 cites W2005945803 @default.
• W206702936 cites W2007313599 @default.
• W206702936 cites W2007726567 @default.
• W206702936 cites W2008693647 @default.
• W206702936 cites W2009173868 @default.
• W206702936 cites W2010864955 @default.
• W206702936 cites W2013027290 @default.
• W206702936 cites W2013125831 @default.
• W206702936 cites W2013212354 @default.
• W206702936 cites W2013857388 @default.
• W206702936 cites W2017481656 @default.
• W206702936 cites W2017536425 @default.
• W206702936 cites W2019044913 @default.
• W206702936 cites W2019728262 @default.
• W206702936 cites W2020879401 @default.
• W206702936 cites W2022240091 @default.
• W206702936 cites W2022357538 @default.
• W206702936 cites W2022555390 @default.
• W206702936 cites W2023751592 @default.
• W206702936 cites W2024727248 @default.
• W206702936 cites W2024984678 @default.

• next