Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067029408> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2067029408 abstract "Intramolecular distance measurements were made in cardiac troponin C (cTnC) by fluorescence energy transfer using Eu3+ or Tb3+ as energy donors and Nd3+ or an organic chromophore as acceptors. The laser-induced luminescence of bound Eu3+ is quenched in Eu1Nd1cTnC with a lifetime of 0.328 ms, compared with 0.43 ms for Eu2cTnC. The enhanced decay corresponds to an energy transfer efficiency of 0.25, or a distance of 1.1 nm between the two high affinity sites. We have also labeled cTnC with 4-dimethylaminophenylazophenyl-4'-maleimide (DAB-Mal) at the two cysteine residues (Cys-35 and Cys-84). Energy transfer measurements were carried out between Tb3+ bound to the high affinity sites and the labels attached to the domain containing the low affinity site. Upon uv irradiation at pH 6.7, Tb1cTnCDAB emits tyrosine-sensitized Tb3+ luminescence that decays bioexponentially with lifetimes of 1.29 and 0.76 ms. The shorter lifetime is ascribed to energy transfer from Tb3+ to the DAB labels, yielding an average distance of 3.4 nm between the donor and the acceptors. At pH 5.0, however, the luminescence decays exclusively with a single lifetime of 1.31 ms, suggesting that under these conditions all Tb3+ ions are more than 5.2 nm away from the label. Thus cTnC, like skeletal TnC, undergoes a pH-dependent conformational transition which converts an elongated structure at lower pH's to a rather compact conformation in a more physiological medium." @default.
- W2067029408 created "2016-06-24" @default.
- W2067029408 creator A5008260140 @default.
- W2067029408 creator A5035521330 @default.
- W2067029408 date "1990-01-01" @default.
- W2067029408 modified "2023-09-25" @default.
- W2067029408 title "Distance measurements in cardiac troponin C" @default.
- W2067029408 cites W1500584371 @default.
- W2067029408 cites W1526906553 @default.
- W2067029408 cites W1531677157 @default.
- W2067029408 cites W1533853543 @default.
- W2067029408 cites W1567044991 @default.
- W2067029408 cites W1575714747 @default.
- W2067029408 cites W1591865251 @default.
- W2067029408 cites W1922195907 @default.
- W2067029408 cites W2040389590 @default.
- W2067029408 cites W2053591536 @default.
- W2067029408 cites W2073883999 @default.
- W2067029408 cites W2088178866 @default.
- W2067029408 cites W2089778751 @default.
- W2067029408 cites W2131970139 @default.
- W2067029408 cites W2163284861 @default.
- W2067029408 cites W3004736022 @default.
- W2067029408 cites W4229717631 @default.
- W2067029408 doi "https://doi.org/10.1016/0003-9861(90)90032-t" @default.
- W2067029408 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2105078" @default.
- W2067029408 hasPublicationYear "1990" @default.
- W2067029408 type Work @default.
- W2067029408 sameAs 2067029408 @default.
- W2067029408 citedByCount "9" @default.
- W2067029408 crossrefType "journal-article" @default.
- W2067029408 hasAuthorship W2067029408A5008260140 @default.
- W2067029408 hasAuthorship W2067029408A5035521330 @default.
- W2067029408 hasConcept C106447425 @default.
- W2067029408 hasConcept C121332964 @default.
- W2067029408 hasConcept C145148216 @default.
- W2067029408 hasConcept C148869448 @default.
- W2067029408 hasConcept C178790620 @default.
- W2067029408 hasConcept C181199279 @default.
- W2067029408 hasConcept C185592680 @default.
- W2067029408 hasConcept C188027245 @default.
- W2067029408 hasConcept C192468462 @default.
- W2067029408 hasConcept C192562407 @default.
- W2067029408 hasConcept C2779201268 @default.
- W2067029408 hasConcept C2780105360 @default.
- W2067029408 hasConcept C41999313 @default.
- W2067029408 hasConcept C49040817 @default.
- W2067029408 hasConcept C55493867 @default.
- W2067029408 hasConcept C62520636 @default.
- W2067029408 hasConcept C71240020 @default.
- W2067029408 hasConcept C75079739 @default.
- W2067029408 hasConcept C75473681 @default.
- W2067029408 hasConcept C8010536 @default.
- W2067029408 hasConcept C91881484 @default.
- W2067029408 hasConcept C96305047 @default.
- W2067029408 hasConceptScore W2067029408C106447425 @default.
- W2067029408 hasConceptScore W2067029408C121332964 @default.
- W2067029408 hasConceptScore W2067029408C145148216 @default.
- W2067029408 hasConceptScore W2067029408C148869448 @default.
- W2067029408 hasConceptScore W2067029408C178790620 @default.
- W2067029408 hasConceptScore W2067029408C181199279 @default.
- W2067029408 hasConceptScore W2067029408C185592680 @default.
- W2067029408 hasConceptScore W2067029408C188027245 @default.
- W2067029408 hasConceptScore W2067029408C192468462 @default.
- W2067029408 hasConceptScore W2067029408C192562407 @default.
- W2067029408 hasConceptScore W2067029408C2779201268 @default.
- W2067029408 hasConceptScore W2067029408C2780105360 @default.
- W2067029408 hasConceptScore W2067029408C41999313 @default.
- W2067029408 hasConceptScore W2067029408C49040817 @default.
- W2067029408 hasConceptScore W2067029408C55493867 @default.
- W2067029408 hasConceptScore W2067029408C62520636 @default.
- W2067029408 hasConceptScore W2067029408C71240020 @default.
- W2067029408 hasConceptScore W2067029408C75079739 @default.
- W2067029408 hasConceptScore W2067029408C75473681 @default.
- W2067029408 hasConceptScore W2067029408C8010536 @default.
- W2067029408 hasConceptScore W2067029408C91881484 @default.
- W2067029408 hasConceptScore W2067029408C96305047 @default.
- W2067029408 hasLocation W20670294081 @default.
- W2067029408 hasLocation W20670294082 @default.
- W2067029408 hasOpenAccess W2067029408 @default.
- W2067029408 hasPrimaryLocation W20670294081 @default.
- W2067029408 hasRelatedWork W1997784010 @default.
- W2067029408 hasRelatedWork W2051373389 @default.
- W2067029408 hasRelatedWork W2052804580 @default.
- W2067029408 hasRelatedWork W2074296394 @default.
- W2067029408 hasRelatedWork W2076051033 @default.
- W2067029408 hasRelatedWork W2089448003 @default.
- W2067029408 hasRelatedWork W2111815411 @default.
- W2067029408 hasRelatedWork W2136713950 @default.
- W2067029408 hasRelatedWork W2653569228 @default.
- W2067029408 hasRelatedWork W2950525397 @default.
- W2067029408 isParatext "false" @default.
- W2067029408 isRetracted "false" @default.
- W2067029408 magId "2067029408" @default.
- W2067029408 workType "article" @default.