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• W2067047487 abstract "CHS (EC 2.3.1.74) catalyzes the condensation of three molecules of malonyl-COA with one molecule of 4-coumaroyl COA to produce 2’,4,4’,6-tetrahydroxychalcone (Heller and Hahlbrock, 1980). The CHS genes have been isolated and characterized in a variety of plants. In the Leguminosae, CHS is a multiple gene family, with subterranean clover (Trifolium subterraneum) having at least nine copies (Arioli et al., 1994). We have recently shown that different copies within this gene family are induced by wounding and Rhizobium infection (Lawson et al., 1994). Screening of a T. subterraneum genomic library with the cDNA clone for CHSZ from bean (Ryder et al., 1987) isolated a number of clones; of these, CHSZ and CHS2 were fully characterized (Arioli et al., 1994). To permit identification of the promoters responsible for the expression of separate gene copies, we determined sequences for additional CHS genes. Sequence data for CHS3 and CHS4, which are linked to CHSZ and CHS2, and CHS5 and CHS6, which, together with a pseudogene CHS7, form a second linkage group, are presented here (Table I). An as-yet unlinked gene, CHS8, has been cloned and partially sequenced to confirm its homology to CHS; this sequence is not presented. The COOH-terminal portion of CHS4 is not located on any of the A clones characterized; thus, the sequence for this gene is truncated 236 bp prior to the end of the gene. Six hundred base pairs of upstream sequence is included in the data base listings for each of these genes. A11 of the CHS sequences within each linkage cluster are oriented in the same direction. The average homology of the six clover CHS genes is 93% at the DNA and 98% at the amino acid level. Curiously, greater homology is present between some members of the two linkage clusters than within each cluster. The phylogenetic relationships be-" @default.
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• W2067047487 date "1995-03-01" @default.
• W2067047487 modified "2023-09-25" @default.
• W2067047487 title "Nucleotide Sequence of Additional Members of the Gene Family Encoding Chalcone Synthase in Trifolium subterraneum" @default.
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• W2067047487 doi "https://doi.org/10.1104/pp.107.3.1035" @default.
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