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- W2067087310 abstract "FACT is a heterodimer of SPT16 and SSRP1, which each contain several conserved regions in the primary structure. The interaction of FACT with nucleosomes induces chromatin remodeling through the combinatorial action of its distinct functional protein regions. However, there is little mechanistic insight into how these regions cooperatively contribute to FACT functions, particularly regarding the recognition of nucleosomal DNA. Here, we report the identification of novel phosphorylation sites of Drosophila melanogaster FACT (dFACT) expressed in Sf9 cells. These sites are densely concentrated in the acidic intrinsically disordered (ID) region of the SSRP1 subunit and control nucleosomal DNA binding by dFACT. This region and the adjacent segment of the HMG domain form weak electrostatic intramolecular interactions, which is reinforced by the phosphorylation, thereby blocking DNA binding competitively. Importantly, this control mechanism appears to support rapid chromatin transactions during early embryogenesis through the dephosphorylation of some sites in the maternally transmitted dSSRP1." @default.
- W2067087310 created "2016-06-24" @default.
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- W2067087310 date "2009-09-01" @default.
- W2067087310 modified "2023-09-26" @default.
- W2067087310 title "Phosphorylated Intrinsically Disordered Region of FACT Masks Its Nucleosomal DNA Binding Elements" @default.
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- W2067087310 doi "https://doi.org/10.1074/jbc.m109.001958" @default.
- W2067087310 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2782050" @default.
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