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- W2067111919 abstract "A simple and sensitive method was developed for the quantification of amikacin in human plasma and urine samples. The method involves centrifugation of body fluid plasma after dilution with an ethanol/sodium carbonate mixture, and then an aliquot of the supernatant is directly injected into the chromatograph. After separation on a reversed-phase C18 column (runtime 20 min), aminoglycoside is detected on the basis of its complex formation reaction with Cu(II), the catalyst of the luminol/hydrogen peroxide chemiluminescence system. Using a volume of 500 microl biological sample, linearity is established over the concentration range 0.15-2.0 microg/ml and the limit of detection (LOD) is ca. 50 microg/l in plasma or urine. The intra-day and inter-day precision (measured by relative standard deviation, R.S.D.%) are always less than 9%, and relative recoveries are found to be over 92%." @default.
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- W2067111919 date "2006-10-01" @default.
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- W2067111919 title "Determination of amikacin in body fluid by high-performance liquid-chromatography with chemiluminescence detection" @default.
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- W2067111919 doi "https://doi.org/10.1016/j.jchromb.2006.05.016" @default.
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