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- W2067144429 abstract "It has recently been reported that insulin-degrading enzyme (IDE) contains an allosteric site which binds polyanions such as ATP and PPPi. This site is distinct from the catalytic site where homotrophic allosteric effects are produced. In this study, we have characterized the binding of ATP to this anion binding site using the fluorescent ATP analog 2',3'-O-(2,4,6-trinitrophenyl)-adenosine triphosphate (TNP-ATP), which exhibits a higher affinity to the enzyme than ATP itself. TNP-ATP binding to IDE was accompanied by a more than 4-fold increase in fluorescence. The dissociation constant (K(D)) of TNP-ATP was determined as 1.15 microM, while the activation constant (K(A)) was determined to be 1.6 microM. Competition experiments were used to show that ATP (Ki = 1.3 mM) and PPPi (Ki = 0.9mM) bind with a higher affinity than ADP (2.2 mM) and AMP (4.0 mM). Adenosine did not bind to the anion binding site." @default.
- W2067144429 created "2016-06-24" @default.
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- W2067144429 date "2006-07-01" @default.
- W2067144429 modified "2023-09-24" @default.
- W2067144429 title "Characterization of the binding of the fluorescent ATP analog TNP-ATP to insulysin" @default.
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- W2067144429 doi "https://doi.org/10.1016/j.abb.2006.04.011" @default.
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