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• W2067221444 startingPage "653" @default.
• W2067221444 abstract "Previous work in our laboratory has shown that microvascular pericytes sort muscle and nonmuscle actin isoforms into discrete cytoplasmic domains (Herman, I. M., and P. A. D'Amore. 1985. J. Cell Biol. 101:43-52; DeNofrio, D.T.C. Hoock, and I. M. Herman. J. Cell. Biol. 109:191-202). Specifically, muscle (alpha-smooth) actin is present on the stress fibers while nonmuscle actins (beta and gamma) are located on stress fibers and in regions of moving cytoplasm (e.g., ruffles, lamellae). To determine the form and function of beta actin in microvascular pericytes and endothelial cells recovering from injury, we prepared isoform-specific antibodies and cDNA probes for immunolocalization, Western and Northern blotting, as well as in situ hybridization. Anti-beta actin IgG was prepared by adsorption and release of beta actin-specific IgG from electrophoretically purified pericyte beta actin bound to nitrocellulose paper. Anti-beta actin IgGs prepared by this affinity selection procedure showed exclusive binding to beta actin present in crude cell lysates containing all three actin isoforms. For controls, we localized beta actin as a bright rim of staining beneath the erythrocyte plasma membrane. Anti-beta actin IgG, absorbed with beta actin bound to nitrocellulose, failed to stain erythrocytes. Simultaneous localization of beta actin with the entire F-actin pool was performed on microvascular pericytes or endothelial cells and 3T3 fibroblasts recovering from injury using anti-beta actin IgG in combination with fluorescent phalloidin. Results of these experiments revealed that pericyte beta actin is localized beneath the plasma membrane in association with filopods, pseudopods, and fan lamellae. Additionally, we observed bright focal fluorescence within fan lamellae and in association with the ends of stress fibers that are preferentially associated with the ventral plasmalemma. Whereas fluorescent phalloidin staining along the stress fibers is continuous, anti-beta actin IgG localization is discontinuous. When injured endothelial and 3T3 cells were stained through wound closure, we localized beta actin only in motile cytoplasm at the wound edge. Staining disappeared as cells became quiescent upon monolayer restoration. Appearance of beta actin at the wound edge correlated with a two- to threefold increase in steady-state levels of beta actin mRNA, which rose within 15-60 min after injury and returned to noninjury levels during monolayer restoration. In situ hybridization revealed that transcripts encoding beta actin were localized at the wound edge in association with the repositioned protein. Results of these experiments indicate that beta actin and its encoded mRNA are polarized at the membrane-cytoskeletal interface within regions of moving cytoplasm." @default.
• W2067221444 created "2016-06-24" @default.
• W2067221444 creator A5006189936 @default.
• W2067221444 creator A5060296456 @default.
• W2067221444 creator A5077822196 @default.
• W2067221444 date "1991-02-15" @default.
• W2067221444 modified "2023-10-15" @default.
• W2067221444 title "Beta actin and its mRNA are localized at the plasma membrane and the regions of moving cytoplasm during the cellular response to injury." @default.
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• W2067221444 cites W1533375024 @default.
• W2067221444 cites W1540784621 @default.
• W2067221444 cites W1544176955 @default.
• W2067221444 cites W1549735471 @default.
• W2067221444 cites W1566845757 @default.
• W2067221444 cites W1602991363 @default.
• W2067221444 cites W1607077699 @default.
• W2067221444 cites W1945199177 @default.
• W2067221444 cites W1964748714 @default.
• W2067221444 cites W1966413318 @default.
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• W2067221444 cites W1972683276 @default.
• W2067221444 cites W1974144015 @default.
• W2067221444 cites W1974423273 @default.
• W2067221444 cites W1976138565 @default.
• W2067221444 cites W1978452865 @default.
• W2067221444 cites W1985476482 @default.
• W2067221444 cites W1993970613 @default.
• W2067221444 cites W2003841601 @default.
• W2067221444 cites W2011756605 @default.
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• W2067221444 cites W2015117134 @default.
• W2067221444 cites W2020867437 @default.
• W2067221444 cites W2028317903 @default.
• W2067221444 cites W2033924280 @default.
• W2067221444 cites W2034876129 @default.
• W2067221444 cites W2038300967 @default.
• W2067221444 cites W2039531420 @default.
• W2067221444 cites W2048210319 @default.
• W2067221444 cites W2049431994 @default.
• W2067221444 cites W2051737308 @default.
• W2067221444 cites W2053484736 @default.
• W2067221444 cites W2064967260 @default.
• W2067221444 cites W2069033493 @default.
• W2067221444 cites W2069886435 @default.
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• W2067221444 cites W2074862264 @default.
• W2067221444 cites W2075478289 @default.
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• W2067221444 cites W2079702620 @default.
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• W2067221444 cites W2102174840 @default.
• W2067221444 cites W2108337314 @default.
• W2067221444 cites W2117832434 @default.
• W2067221444 cites W2118449156 @default.
• W2067221444 cites W2122805183 @default.
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• W2067221444 cites W2129269025 @default.
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• W2067221444 doi "https://doi.org/10.1083/jcb.112.4.653" @default.
• W2067221444 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2288855" @default.
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• W2067221444 hasPublicationYear "1991" @default.
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