Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067293048> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2067293048 endingPage "207" @default.
- W2067293048 startingPage "197" @default.
- W2067293048 abstract "The third enzyme of the pentose phosphate pathway (PPP), 6-phosphogluconate dehydrogenase (6PGDH), is present in the four major stages of Trypanosoma cruzi, CL Brener clone. The enzyme was too unstable to be purified from epimastigote cell-free extracts. Two genes encoding 6PGDH were cloned and sequenced; the predicted amino acid sequences differ only in five non-essential residues. Since Southern blots suggested the presence of a single copy per haploid genome, the two genes found are probably alleles. One of these genes, encoding a protein with 78.6% identity with the Trypanosoma brucei 6PGDH, was expressed in Escherichia coli as an active recombinant enzyme, which was as unstable as the native 6PGDH. Modeling of the T. cruzi enzyme using the three-dimensional structure of the T. brucei 6PGDH as template suggested the lack of two out of five salt bridges proposed to strengthen subunit interactions in the active dimer. Restoring of these bridges by site-directed mutagenesis resulted in a more stable recombinant T. cruzi 6PGDH, which was used to determine the kinetic parameters. The K(m) value for 6-phosphogluconate (22.2+/-0.4 microM) was identical to the values reported for 6PGDHs from mammals, but the K(m) for NADP (5.9+/-0.2 microM) was significantly lower than the value reported for the human enzyme, and closer to that for the T. brucei enzyme. This suggests the possibility that inhibitors of the T. brucei 6PGDH, under development as potential drugs against African Trypanosomiasis, might also be successful for the chemotherapy of Chagas disease." @default.
- W2067293048 created "2016-06-24" @default.
- W2067293048 creator A5049091535 @default.
- W2067293048 creator A5080380194 @default.
- W2067293048 date "2004-02-01" @default.
- W2067293048 modified "2023-10-06" @default.
- W2067293048 title "The 6-phosphogluconate dehydrogenase from Trypanosoma cruzi: the absence of two inter-subunit salt bridges as a reason for enzyme instability" @default.
- W2067293048 cites W1203072618 @default.
- W2067293048 cites W1539428981 @default.
- W2067293048 cites W1625660792 @default.
- W2067293048 cites W1853891491 @default.
- W2067293048 cites W1869944834 @default.
- W2067293048 cites W1966142862 @default.
- W2067293048 cites W1972207411 @default.
- W2067293048 cites W2003589493 @default.
- W2067293048 cites W2009523353 @default.
- W2067293048 cites W2013732161 @default.
- W2067293048 cites W2015642465 @default.
- W2067293048 cites W2021822101 @default.
- W2067293048 cites W2032349840 @default.
- W2067293048 cites W2038592624 @default.
- W2067293048 cites W2049220240 @default.
- W2067293048 cites W2060676205 @default.
- W2067293048 cites W2069715420 @default.
- W2067293048 cites W2072440135 @default.
- W2067293048 cites W2083367088 @default.
- W2067293048 cites W2083803721 @default.
- W2067293048 cites W2093302608 @default.
- W2067293048 cites W2140351370 @default.
- W2067293048 cites W2158714788 @default.
- W2067293048 cites W4256331271 @default.
- W2067293048 cites W4293247451 @default.
- W2067293048 doi "https://doi.org/10.1016/j.molbiopara.2003.10.007" @default.
- W2067293048 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14698432" @default.
- W2067293048 hasPublicationYear "2004" @default.
- W2067293048 type Work @default.
- W2067293048 sameAs 2067293048 @default.
- W2067293048 citedByCount "23" @default.
- W2067293048 countsByYear W20672930482013 @default.
- W2067293048 countsByYear W20672930482016 @default.
- W2067293048 countsByYear W20672930482017 @default.
- W2067293048 countsByYear W20672930482018 @default.
- W2067293048 countsByYear W20672930482019 @default.
- W2067293048 countsByYear W20672930482020 @default.
- W2067293048 countsByYear W20672930482021 @default.
- W2067293048 countsByYear W20672930482022 @default.
- W2067293048 countsByYear W20672930482023 @default.
- W2067293048 crossrefType "journal-article" @default.
- W2067293048 hasAuthorship W2067293048A5049091535 @default.
- W2067293048 hasAuthorship W2067293048A5080380194 @default.
- W2067293048 hasConcept C104292427 @default.
- W2067293048 hasConcept C104317684 @default.
- W2067293048 hasConcept C136764020 @default.
- W2067293048 hasConcept C153911025 @default.
- W2067293048 hasConcept C181199279 @default.
- W2067293048 hasConcept C2776317432 @default.
- W2067293048 hasConcept C2777625466 @default.
- W2067293048 hasConcept C2779502636 @default.
- W2067293048 hasConcept C40767141 @default.
- W2067293048 hasConcept C41008148 @default.
- W2067293048 hasConcept C55493867 @default.
- W2067293048 hasConcept C71928629 @default.
- W2067293048 hasConcept C86803240 @default.
- W2067293048 hasConceptScore W2067293048C104292427 @default.
- W2067293048 hasConceptScore W2067293048C104317684 @default.
- W2067293048 hasConceptScore W2067293048C136764020 @default.
- W2067293048 hasConceptScore W2067293048C153911025 @default.
- W2067293048 hasConceptScore W2067293048C181199279 @default.
- W2067293048 hasConceptScore W2067293048C2776317432 @default.
- W2067293048 hasConceptScore W2067293048C2777625466 @default.
- W2067293048 hasConceptScore W2067293048C2779502636 @default.
- W2067293048 hasConceptScore W2067293048C40767141 @default.
- W2067293048 hasConceptScore W2067293048C41008148 @default.
- W2067293048 hasConceptScore W2067293048C55493867 @default.
- W2067293048 hasConceptScore W2067293048C71928629 @default.
- W2067293048 hasConceptScore W2067293048C86803240 @default.
- W2067293048 hasIssue "2" @default.
- W2067293048 hasLocation W20672930481 @default.
- W2067293048 hasLocation W20672930482 @default.
- W2067293048 hasOpenAccess W2067293048 @default.
- W2067293048 hasPrimaryLocation W20672930481 @default.
- W2067293048 hasRelatedWork W1535521978 @default.
- W2067293048 hasRelatedWork W1853891491 @default.
- W2067293048 hasRelatedWork W1983131282 @default.
- W2067293048 hasRelatedWork W2001016396 @default.
- W2067293048 hasRelatedWork W2021143980 @default.
- W2067293048 hasRelatedWork W2048288137 @default.
- W2067293048 hasRelatedWork W2064042198 @default.
- W2067293048 hasRelatedWork W2078646031 @default.
- W2067293048 hasRelatedWork W2128957355 @default.
- W2067293048 hasRelatedWork W35790772 @default.
- W2067293048 hasVolume "133" @default.
- W2067293048 isParatext "false" @default.
- W2067293048 isRetracted "false" @default.
- W2067293048 magId "2067293048" @default.
- W2067293048 workType "article" @default.