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• W2067392246 abstract "The effect of some ammonium salts on nitrate reductase (NR) level, onin vivo nitrate reduction and on nitrate content was followed in the presence of nitrate in the medium, under changing experimental conditions, in excisedPisum sativum roots, and their effect was compared with that of KNO3, Ca(NO3)2 and NaNO3 at 15 mM NO3 - concentration, i.e. at a concentration which considerably exceeded the level of saturation with nitrate with respect to nitrate reductase. The effect of ammonium salts on NR level is indirect and changes from a positive one to a strongly negative one which is dependent on the time of action of the salt, on the presence of other cations, on pH of the solution of the ammonium salt and on the nature of the anion of the ammonium salt. A positive effect on the enzyme level can be observed in the presence of other cations than NH4 + at suitable concentrations of those ammonium salts, the solutions of which have their pH values in the acid region (i.e. NH4H2PO4, (NH4)2SO4 and NH4NO3). However their positive effect is independent of the presence of NH4 + ions, and it is obviously the result of an increased concentration of H+ ions. A clear-cut negative effect on NR level can be observed after 24 h in one-salt NH4NO3 solution where NH4 + is not balanced with other cations and thus certainly can adversely influence many metabolic processes, and in the solutions containing neutral (pH 6.2) and dibasic ammonium phosphates in which dissolved undissociated ammonia [(NH3). (H2O) which can also affect many metabolic processes incl. proteosynthesis] probably has a toxic influence. Thein vivo nitrate reduction is always depressed in excised pea roots in the presence of ammonium salts in the medium, regardless of the level of nitrate reductase. Under the described conditions, no relationship could be established between the enzyme level and the so-called metabolic NO3 - pool (i.e. NO2 - production under anaerobic conditions), nor between NR level and the total nitrate content in the roots. One-salt solutions of NaNO3, Ca(NO3)2 and KNO3 exert different effects on the level of nitrate reductase and on the content of NO3 - in the roots, but the in vivo NO3 - reduction shows the same trend as NR level in the roots influenced by these salts. Cl- ions, supplied in NH4C1, depress both NR level and NO3 - content in the roots at higher concentrations, but they do not significantly affect the in vivo nitrate reduction in comparison with other ammonium salts. These results indicate that NR level,in vivo nitrate reduction, and nitrate uptake can be regulated in pea roots independently of each other." @default.
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• W2067392246 date "1977-03-01" @default.
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• W2067392246 title "The effect of some ammonium salts on nitrate reductase level, onin vivo nitrate reduction and on nitrate content in excisedpisum sativum roots" @default.
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