FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067417775> ?p ?o ?g. }

• W2067417775 endingPage "140" @default.
• W2067417775 startingPage "131" @default.
• W2067417775 abstract "Endothelin-1 (ET-1) is both a potent vasoconstrictor and mitogenic factor that has been implicated as a cause of the micro- and macrovascular complications of diabetes mellitus. The pathway by which the high-glucose environment of diabetes mediates increased levels of endothelins has not been completely elucidated but appears to involve endothelin-converting enzyme (ECE-1), which converts inactive big ET-1 to active ET-1 peptide. To determine the effect of high glucose concentrations on the expression of ECE-1, hybrid endothelial cells (EA.hy926) and human umbilical vein endothelial cells (HUVEC) were both grown in various glucose concentrations. There was a 2-fold increase in ECE-1 immunoreactivity in the EA.hy926 cell line growing in medium containing 22.2 versus 5.5 mmol/l glucose after 24 h, which rose to greater than 20-fold after 5 days. Similar results were seen with HUVEC. Bradykinin or NG-nitro-L-arginine methyl ester did not change the effect of high glucose on ECE-1 protein expression. High glucose induced a 72 and 41% increase in total protein kinase C (PKC) activity in both EA.hy926 cells and HUVEC, respectively, and a 39, 49 and 109% elevation in PKC beta1, beta2 and delta expression, respectively, in EA.hy926 cells. The increase in ECE-1 expression was inhibited in both cell cultures by GF109203X (5 micromol/l), a general PKC inhibitor, while addition of 10 nmol/l phorbol myristic acid to EA.hy926 cells or HUVEC growing on medium containing 5.5 mmol/l glucose increased ECE-1 expression to a level similar to that of cells conditioned in high glucose. Human ECE-1 protein exists in four different isoforms, termed 1a, 1b, 1c and 1d. Northern blot analysis revealed that only ECE-1c isoform mRNA levels increased. Immunohistochemical staining of EA.hy926 cells grown in high glucose concentrations demonstrated an increase in the ECE-1c isoform, which occurred mainly in the plasma membrane. These results showed that the PKC pathway may play an important role in the glucose-mediated induction of ECE-1 expression. The main isoform to increase in response to high glucose was ECE-1c. This enzyme may be one of the factors contributing to the elevated ET-1 peptide levels observed in diabetes." @default.
• W2067417775 created "2016-06-24" @default.
• W2067417775 creator A5006230964 @default.
• W2067417775 creator A5014012711 @default.
• W2067417775 creator A5018464917 @default.
• W2067417775 creator A5034035931 @default.
• W2067417775 creator A5056475417 @default.
• W2067417775 creator A5062334405 @default.
• W2067417775 creator A5073517018 @default.
• W2067417775 date "2004-01-01" @default.
• W2067417775 modified "2023-10-15" @default.
• W2067417775 title "Increased Expression of Endothelin-Converting Enzyme-1c Isoform in Response to High Glucose Levels in Endothelial Cells" @default.
• W2067417775 cites W1504236746 @default.
• W2067417775 cites W1988887575 @default.
• W2067417775 cites W2001381510 @default.
• W2067417775 cites W2008580181 @default.
• W2067417775 cites W2014605254 @default.
• W2067417775 cites W2017054020 @default.
• W2067417775 cites W2033138908 @default.
• W2067417775 cites W2039383811 @default.
• W2067417775 cites W2050853912 @default.
• W2067417775 cites W2059369537 @default.
• W2067417775 cites W2064324181 @default.
• W2067417775 cites W2066010552 @default.
• W2067417775 cites W2069009397 @default.
• W2067417775 cites W2070580300 @default.
• W2067417775 cites W2074669651 @default.
• W2067417775 cites W2082953465 @default.
• W2067417775 cites W2092842648 @default.
• W2067417775 cites W2110439479 @default.
• W2067417775 cites W2151869895 @default.
• W2067417775 cites W2162804111 @default.
• W2067417775 cites W2167700163 @default.
• W2067417775 doi "https://doi.org/10.1159/000077132" @default.
• W2067417775 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15010576" @default.
• W2067417775 hasPublicationYear "2004" @default.
• W2067417775 type Work @default.
• W2067417775 sameAs 2067417775 @default.
• W2067417775 citedByCount "44" @default.
• W2067417775 countsByYear W20674177752012 @default.
• W2067417775 countsByYear W20674177752013 @default.
• W2067417775 countsByYear W20674177752014 @default.
• W2067417775 countsByYear W20674177752015 @default.
• W2067417775 countsByYear W20674177752016 @default.
• W2067417775 countsByYear W20674177752017 @default.
• W2067417775 countsByYear W20674177752019 @default.
• W2067417775 countsByYear W20674177752020 @default.
• W2067417775 crossrefType "journal-article" @default.
• W2067417775 hasAuthorship W2067417775A5006230964 @default.
• W2067417775 hasAuthorship W2067417775A5014012711 @default.
• W2067417775 hasAuthorship W2067417775A5018464917 @default.
• W2067417775 hasAuthorship W2067417775A5034035931 @default.
• W2067417775 hasAuthorship W2067417775A5056475417 @default.
• W2067417775 hasAuthorship W2067417775A5062334405 @default.
• W2067417775 hasAuthorship W2067417775A5073517018 @default.
• W2067417775 hasConcept C123012128 @default.
• W2067417775 hasConcept C126322002 @default.
• W2067417775 hasConcept C134018914 @default.
• W2067417775 hasConcept C144980905 @default.
• W2067417775 hasConcept C149601957 @default.
• W2067417775 hasConcept C165220095 @default.
• W2067417775 hasConcept C170493617 @default.
• W2067417775 hasConcept C181199279 @default.
• W2067417775 hasConcept C185592680 @default.
• W2067417775 hasConcept C195794163 @default.
• W2067417775 hasConcept C202751555 @default.
• W2067417775 hasConcept C2777411675 @default.
• W2067417775 hasConcept C2779591629 @default.
• W2067417775 hasConcept C2780043322 @default.
• W2067417775 hasConcept C2780963292 @default.
• W2067417775 hasConcept C2909649922 @default.
• W2067417775 hasConcept C54355233 @default.
• W2067417775 hasConcept C55493867 @default.
• W2067417775 hasConcept C555293320 @default.
• W2067417775 hasConcept C71924100 @default.
• W2067417775 hasConcept C81885089 @default.
• W2067417775 hasConcept C86803240 @default.
• W2067417775 hasConceptScore W2067417775C123012128 @default.
• W2067417775 hasConceptScore W2067417775C126322002 @default.
• W2067417775 hasConceptScore W2067417775C134018914 @default.
• W2067417775 hasConceptScore W2067417775C144980905 @default.
• W2067417775 hasConceptScore W2067417775C149601957 @default.
• W2067417775 hasConceptScore W2067417775C165220095 @default.
• W2067417775 hasConceptScore W2067417775C170493617 @default.
• W2067417775 hasConceptScore W2067417775C181199279 @default.
• W2067417775 hasConceptScore W2067417775C185592680 @default.
• W2067417775 hasConceptScore W2067417775C195794163 @default.
• W2067417775 hasConceptScore W2067417775C202751555 @default.
• W2067417775 hasConceptScore W2067417775C2777411675 @default.
• W2067417775 hasConceptScore W2067417775C2779591629 @default.
• W2067417775 hasConceptScore W2067417775C2780043322 @default.
• W2067417775 hasConceptScore W2067417775C2780963292 @default.
• W2067417775 hasConceptScore W2067417775C2909649922 @default.
• W2067417775 hasConceptScore W2067417775C54355233 @default.
• W2067417775 hasConceptScore W2067417775C55493867 @default.
• W2067417775 hasConceptScore W2067417775C555293320 @default.
• W2067417775 hasConceptScore W2067417775C71924100 @default.
• W2067417775 hasConceptScore W2067417775C81885089 @default.

• next