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• W2067432815 abstract "Background We have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs. Methodology/Principal Findings The method uses tagged priming and template-switching, resulting in the incorporation of universal PCR priming sites at both ends of the synthesized cDNA for global PCR amplification. Coupled with a whole-genome gene expression microarray platform, we routinely obtain expression correlation values of R2∼0.76–0.80 between individual cells and R2∼0.69 between 50 pg total RNA replicates. Expression profiles generated from single cells or 50 pg total RNA correlate well with that generated with higher input (1 ng total RNA) (R2∼0.80). Also, the assay is sufficiently sensitive to detect, in a single cell, approximately 63% of the number of genes detected with 1 ng input, with approximately 97% of the genes detected in the single-cell input also detected in the higher input. Conclusions/Significance In summary, our method facilitates whole-genome gene expression profiling in contexts where starting material is extremely limiting, particularly in areas such as the study of progenitor cells in early development and tumor stem cell biology." @default.
• W2067432815 created "2016-06-24" @default.
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• W2067432815 date "2012-02-08" @default.
• W2067432815 modified "2023-10-01" @default.
• W2067432815 title "Highly Parallel Genome-Wide Expression Analysis of Single Mammalian Cells" @default.
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• W2067432815 doi "https://doi.org/10.1371/journal.pone.0030794" @default.
• W2067432815 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3275609" @default.
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• W2067432815 hasPublicationYear "2012" @default.
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