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- W2067495204 abstract "Studies have been made with isolated chloroplasts using a new amperometric method which allows us to measure the activities of Photosystems I and II separately or cooperating in enhancement and chromatic transients. 1. The occurrence of the latter phenomena in isolated chloroplasts could be clearly established. 2. The action spectra of the two photosystems and the ratio between these two have been measured with precision. 3. The action spectrum of System I proved to be independent of the state of the traps of System II; this demonstrated absence of photon transfer from pigments of System II to pigments of System I (spillover). 4. The dependence of the rate upon the concentration of active trapping centers has been studied for each photosystem. In the case of Photosystem II, the same hyperbolic relation was found which was observed earlier with whole Chlorella cells. In contrast, in Photosystem I the relation between the rate and the concentration of active P−700 proved to be strictly linear. 5. The steady-state concentration of open trapping centers of System I and System II (P− and E+) has been measured in weak light as a function of wavelength. The results indicated that the overall reaction, in which the active forms of the two photoconverters are regenerated, has a low equilibrium constant K(K = 3−10). This low value implies: (a) a small loss of free energy in the dark reactions which connect the two photoacts, (b) a relatively weak dependence of the overall quantum yield upon the light distribution between the two photoacts and (c) a restraint of the maximum quantum yield of the overall process." @default.
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- W2067495204 date "1968-04-01" @default.
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- W2067495204 title "Analysis of the interactions between the two photosystems in isolated chloroplasts" @default.
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- W2067495204 doi "https://doi.org/10.1016/0005-2728(68)90191-6" @default.
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