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• W2067505451 abstract "Abstract The development of nanostructured electrodes for electrochemical biosensors is of significant interest for modern detection, portable devices, and enhanced performance. However, development of such sensors still remains challenging due to the time-consuming, detriment-to-nature, and costly modifications of both electrodes and enzymes. In this work, we report a simple one-step approach to fabricating high-performance, direct electron transfer (DET) based nanoporous enzyme-embedded electrodes by electrodeposition coupled with recent progress in potential-controlled interfacial surfactant assemblies. In contrast to those previously electrodeposited mesoporous materials that are not bioactive, we imparted the biofunctionality to electrodeposited mesoporous thin films by means of the amphiphilic phospholipid templates strongly interacting with enzymes. Thus, phospholipid-templated mesoporous ZnO films covalently inlaid with the pristine enzymes were prepared by simple one-step electrodeposition. We further demonstrate two examples of such hybrid film electrodes embedded with alcohol dehydrogenase (ADH) and glucose oxidase (GOx), which are effectively employed as electrochemical biosensors for amperometric sensing of ethanol and glucose without using any electron relays. The favorable mass transport and large contact surface area provided by nanopores play an important role in improving the performance of these two biosensors, such as excellent sensitivities, low detection limits, and fast response. The matrix mesoporous films acting as effective electronic bridges are responsible for DET between enzyme molecules and metal electrode." @default.
• W2067505451 created "2016-06-24" @default.
• W2067505451 creator A5052072560 @default.
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• W2067505451 date "2014-01-01" @default.
• W2067505451 modified "2023-10-03" @default.
• W2067505451 title "Electrodeposition of enzymes-integrated mesoporous composite films by interfacial templating: A paradigm for electrochemical biosensors" @default.
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• W2067505451 doi "https://doi.org/10.1016/j.electacta.2013.11.088" @default.
• W2067505451 hasPublicationYear "2014" @default.
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