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• W2067510176 abstract "Wilson disease is a disorder of copper metabolism, due to inherited mutations in the Wilson copper ATPase gene ATP7B. To purify and study the function of the ATPase, the enzyme was truncated by five of the six metal binding domains and endowed with an N-terminal histidine-tag for affinity purification. This construct, delta1-5WNDP, was able to functionally complement a yeast strain defective in its native copper ATPase CCC2. Delta1-5WNDP was purified by Ni-affinity chromatography and reconstituted into proteoliposomes. This allowed, for the first time, the functional study of the Wilson ATPase in a purified, reconstituted system." @default.
• W2067510176 created "2016-06-24" @default.
• W2067510176 creator A5073996154 @default.
• W2067510176 creator A5082539064 @default.
• W2067510176 date "2005-06-08" @default.
• W2067510176 modified "2023-10-16" @default.
• W2067510176 title "Purification and functional reconstitution of the human Wilson copper ATPase, ATP7B" @default.
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• W2067510176 doi "https://doi.org/10.1016/j.febslet.2005.05.042" @default.
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