Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067538834> ?p ?o ?g. }
- W2067538834 endingPage "27212" @default.
- W2067538834 startingPage "27206" @default.
- W2067538834 abstract "cDNA clones encoding proteins related to the aggrecan/versican family of proteoglycan core proteins have been isolated with antisera against rat brain synaptic junctions. Two sets of overlapping cDNAs have been characterized that differ in their 3′-terminal regions. Northern analyses with probes derived from unique regions of each set were found to hybridize with two brain-specific transcripts of 3.3 and 3.6 kilobases (kb). The 3.6-kb transcript encodes a polypeptide that exhibits 82% sequence identity with bovine brevican and is thought to be the rat ortholog of brevican. Interestingly, the polypeptide deduced from the open reading frame of the 3.3-kb transcript is truncated just carboxyl-terminal of the central domain of brevican and instead contains a putative glypiation signal. Antibodies raised against a bacterially expressed glutathione S-transferase-brevican fusion protein have been used to show that both soluble and membrane-bound brevican isoforms exist. Treatment of the crude membrane fraction and purified synaptic plasma membranes with phosphatidylinositol-specific phospholipase C revealed that isoforms of brevican are indeed glycosylphosphatidylinositol-anchored to the plasma membrane. Moreover, digestions with chondroitinase ABC have indicated that rat brevican, like its bovine ortholog, is a conditional chondroitin sulfate proteoglycan. Immunohistochemical studies have shown that brevican is widely distributed in the brain and is localized extracellularly. During postnatal development, amounts of both soluble and phosphatidylinositol-specific phospholipase C-sensitive isoforms increase, suggesting a role for brevican in the terminally differentiating and the adult nervous system. cDNA clones encoding proteins related to the aggrecan/versican family of proteoglycan core proteins have been isolated with antisera against rat brain synaptic junctions. Two sets of overlapping cDNAs have been characterized that differ in their 3′-terminal regions. Northern analyses with probes derived from unique regions of each set were found to hybridize with two brain-specific transcripts of 3.3 and 3.6 kilobases (kb). The 3.6-kb transcript encodes a polypeptide that exhibits 82% sequence identity with bovine brevican and is thought to be the rat ortholog of brevican. Interestingly, the polypeptide deduced from the open reading frame of the 3.3-kb transcript is truncated just carboxyl-terminal of the central domain of brevican and instead contains a putative glypiation signal. Antibodies raised against a bacterially expressed glutathione S-transferase-brevican fusion protein have been used to show that both soluble and membrane-bound brevican isoforms exist. Treatment of the crude membrane fraction and purified synaptic plasma membranes with phosphatidylinositol-specific phospholipase C revealed that isoforms of brevican are indeed glycosylphosphatidylinositol-anchored to the plasma membrane. Moreover, digestions with chondroitinase ABC have indicated that rat brevican, like its bovine ortholog, is a conditional chondroitin sulfate proteoglycan. Immunohistochemical studies have shown that brevican is widely distributed in the brain and is localized extracellularly. During postnatal development, amounts of both soluble and phosphatidylinositol-specific phospholipase C-sensitive isoforms increase, suggesting a role for brevican in the terminally differentiating and the adult nervous system." @default.
- W2067538834 created "2016-06-24" @default.
- W2067538834 creator A5008631938 @default.
- W2067538834 creator A5013943166 @default.
- W2067538834 creator A5018076592 @default.
- W2067538834 creator A5057678663 @default.
- W2067538834 creator A5084059039 @default.
- W2067538834 creator A5088206488 @default.
- W2067538834 date "1995-11-01" @default.
- W2067538834 modified "2023-10-16" @default.
- W2067538834 title "Brevican, a Chondroitin Sulfate Proteoglycan of Rat Brain, Occurs as Secreted and Cell Surface Glycosylphosphatidylinositol-anchored Isoforms" @default.
- W2067538834 cites W1484703368 @default.
- W2067538834 cites W1486144458 @default.
- W2067538834 cites W1489215358 @default.
- W2067538834 cites W1500109073 @default.
- W2067538834 cites W1503392039 @default.
- W2067538834 cites W1521949639 @default.
- W2067538834 cites W1531779321 @default.
- W2067538834 cites W1564232667 @default.
- W2067538834 cites W1571924012 @default.
- W2067538834 cites W1573907761 @default.
- W2067538834 cites W1575127176 @default.
- W2067538834 cites W1579361735 @default.
- W2067538834 cites W1581190406 @default.
- W2067538834 cites W1605014062 @default.
- W2067538834 cites W1641143046 @default.
- W2067538834 cites W1656557885 @default.
- W2067538834 cites W1836449346 @default.
- W2067538834 cites W1871282613 @default.
- W2067538834 cites W1979494505 @default.
- W2067538834 cites W1979518563 @default.
- W2067538834 cites W2003782406 @default.
- W2067538834 cites W2012224236 @default.
- W2067538834 cites W2015886388 @default.
- W2067538834 cites W2051217398 @default.
- W2067538834 cites W2060431203 @default.
- W2067538834 cites W2076782206 @default.
- W2067538834 cites W2078455360 @default.
- W2067538834 cites W2082775739 @default.
- W2067538834 cites W2086679033 @default.
- W2067538834 cites W2094886543 @default.
- W2067538834 cites W2144155742 @default.
- W2067538834 cites W2145492711 @default.
- W2067538834 cites W2150531124 @default.
- W2067538834 cites W2170385301 @default.
- W2067538834 cites W2175428614 @default.
- W2067538834 cites W2337841392 @default.
- W2067538834 cites W2413388819 @default.
- W2067538834 cites W5695444 @default.
- W2067538834 doi "https://doi.org/10.1074/jbc.270.45.27206" @default.
- W2067538834 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7592978" @default.
- W2067538834 hasPublicationYear "1995" @default.
- W2067538834 type Work @default.
- W2067538834 sameAs 2067538834 @default.
- W2067538834 citedByCount "135" @default.
- W2067538834 countsByYear W20675388342012 @default.
- W2067538834 countsByYear W20675388342013 @default.
- W2067538834 countsByYear W20675388342014 @default.
- W2067538834 countsByYear W20675388342015 @default.
- W2067538834 countsByYear W20675388342016 @default.
- W2067538834 countsByYear W20675388342017 @default.
- W2067538834 countsByYear W20675388342018 @default.
- W2067538834 countsByYear W20675388342019 @default.
- W2067538834 countsByYear W20675388342020 @default.
- W2067538834 countsByYear W20675388342021 @default.
- W2067538834 countsByYear W20675388342022 @default.
- W2067538834 countsByYear W20675388342023 @default.
- W2067538834 crossrefType "journal-article" @default.
- W2067538834 hasAuthorship W2067538834A5008631938 @default.
- W2067538834 hasAuthorship W2067538834A5013943166 @default.
- W2067538834 hasAuthorship W2067538834A5018076592 @default.
- W2067538834 hasAuthorship W2067538834A5057678663 @default.
- W2067538834 hasAuthorship W2067538834A5084059039 @default.
- W2067538834 hasAuthorship W2067538834A5088206488 @default.
- W2067538834 hasBestOaLocation W20675388341 @default.
- W2067538834 hasConcept C104317684 @default.
- W2067538834 hasConcept C10858879 @default.
- W2067538834 hasConcept C142724271 @default.
- W2067538834 hasConcept C153911025 @default.
- W2067538834 hasConcept C159470946 @default.
- W2067538834 hasConcept C167625842 @default.
- W2067538834 hasConcept C187882448 @default.
- W2067538834 hasConcept C189165786 @default.
- W2067538834 hasConcept C197534660 @default.
- W2067538834 hasConcept C204787440 @default.
- W2067538834 hasConcept C2776164576 @default.
- W2067538834 hasConcept C2779335624 @default.
- W2067538834 hasConcept C3020332539 @default.
- W2067538834 hasConcept C53345823 @default.
- W2067538834 hasConcept C55493867 @default.
- W2067538834 hasConcept C71924100 @default.
- W2067538834 hasConcept C86803240 @default.
- W2067538834 hasConceptScore W2067538834C104317684 @default.
- W2067538834 hasConceptScore W2067538834C10858879 @default.
- W2067538834 hasConceptScore W2067538834C142724271 @default.
- W2067538834 hasConceptScore W2067538834C153911025 @default.
- W2067538834 hasConceptScore W2067538834C159470946 @default.
- W2067538834 hasConceptScore W2067538834C167625842 @default.