Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067545138> ?p ?o ?g. }
- W2067545138 endingPage "18790" @default.
- W2067545138 startingPage "18785" @default.
- W2067545138 abstract "Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is a crucial enzyme in carbon fixation and the most abundant protein on earth. It has been studied extensively by biochemical and structural methods; however, the most essential activation step has not yet been described. Here, we describe the mechanistic details of Lys carbamylation that leads to RuBisCO activation by atmospheric CO 2 . We report two crystal structures of nitrosylated RuBisCO from the red algae Galdieria sulphuraria with O 2 and CO 2 bound at the active site. G. sulphuraria RuBisCO is inhibited by cysteine nitrosylation that results in trapping of these gaseous ligands. The structure with CO 2 defines an elusive, preactivation complex that contains a metal cation Mg 2+ surrounded by three H 2 O/OH molecules. Both structures suggest the mechanism for discriminating gaseous ligands by their quadrupole electric moments. We describe conformational changes that allow for intermittent binding of the metal ion required for activation. On the basis of these structures we propose the individual steps of the activation mechanism. Knowledge of all these elements is indispensable for engineering RuBisCO into a more efficient enzyme for crop enhancement or as a remedy to global warming." @default.
- W2067545138 created "2016-06-24" @default.
- W2067545138 creator A5084942532 @default.
- W2067545138 date "2012-10-29" @default.
- W2067545138 modified "2023-10-14" @default.
- W2067545138 title "Structural mechanism of RuBisCO activation by carbamylation of the active site lysine" @default.
- W2067545138 cites W1501257052 @default.
- W2067545138 cites W1529749143 @default.
- W2067545138 cites W1872795965 @default.
- W2067545138 cites W1966334222 @default.
- W2067545138 cites W1974252038 @default.
- W2067545138 cites W2001347412 @default.
- W2067545138 cites W2009837970 @default.
- W2067545138 cites W2014448529 @default.
- W2067545138 cites W2014881533 @default.
- W2067545138 cites W2030642428 @default.
- W2067545138 cites W2032409213 @default.
- W2067545138 cites W2034830756 @default.
- W2067545138 cites W2044821751 @default.
- W2067545138 cites W2046927023 @default.
- W2067545138 cites W2051798497 @default.
- W2067545138 cites W2056146784 @default.
- W2067545138 cites W2066485538 @default.
- W2067545138 cites W20712287 @default.
- W2067545138 cites W2075236534 @default.
- W2067545138 cites W2098840374 @default.
- W2067545138 cites W2117171098 @default.
- W2067545138 cites W2123682179 @default.
- W2067545138 cites W2125073348 @default.
- W2067545138 cites W2128634572 @default.
- W2067545138 cites W2132174235 @default.
- W2067545138 cites W2136026683 @default.
- W2067545138 cites W2140756273 @default.
- W2067545138 cites W2143576387 @default.
- W2067545138 cites W2147539569 @default.
- W2067545138 cites W2148929113 @default.
- W2067545138 cites W2156766105 @default.
- W2067545138 cites W2157081960 @default.
- W2067545138 cites W2162843192 @default.
- W2067545138 cites W4234475175 @default.
- W2067545138 doi "https://doi.org/10.1073/pnas.1210754109" @default.
- W2067545138 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3503183" @default.
- W2067545138 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23112176" @default.
- W2067545138 hasPublicationYear "2012" @default.
- W2067545138 type Work @default.
- W2067545138 sameAs 2067545138 @default.
- W2067545138 citedByCount "79" @default.
- W2067545138 countsByYear W20675451382013 @default.
- W2067545138 countsByYear W20675451382014 @default.
- W2067545138 countsByYear W20675451382015 @default.
- W2067545138 countsByYear W20675451382016 @default.
- W2067545138 countsByYear W20675451382017 @default.
- W2067545138 countsByYear W20675451382018 @default.
- W2067545138 countsByYear W20675451382019 @default.
- W2067545138 countsByYear W20675451382020 @default.
- W2067545138 countsByYear W20675451382021 @default.
- W2067545138 countsByYear W20675451382022 @default.
- W2067545138 countsByYear W20675451382023 @default.
- W2067545138 crossrefType "journal-article" @default.
- W2067545138 hasAuthorship W2067545138A5084942532 @default.
- W2067545138 hasBestOaLocation W20675451381 @default.
- W2067545138 hasConcept C12554922 @default.
- W2067545138 hasConcept C181199279 @default.
- W2067545138 hasConcept C185592680 @default.
- W2067545138 hasConcept C2777339483 @default.
- W2067545138 hasConcept C2779966244 @default.
- W2067545138 hasConcept C41183919 @default.
- W2067545138 hasConcept C47701112 @default.
- W2067545138 hasConcept C50212798 @default.
- W2067545138 hasConcept C55493867 @default.
- W2067545138 hasConcept C71240020 @default.
- W2067545138 hasConcept C86803240 @default.
- W2067545138 hasConceptScore W2067545138C12554922 @default.
- W2067545138 hasConceptScore W2067545138C181199279 @default.
- W2067545138 hasConceptScore W2067545138C185592680 @default.
- W2067545138 hasConceptScore W2067545138C2777339483 @default.
- W2067545138 hasConceptScore W2067545138C2779966244 @default.
- W2067545138 hasConceptScore W2067545138C41183919 @default.
- W2067545138 hasConceptScore W2067545138C47701112 @default.
- W2067545138 hasConceptScore W2067545138C50212798 @default.
- W2067545138 hasConceptScore W2067545138C55493867 @default.
- W2067545138 hasConceptScore W2067545138C71240020 @default.
- W2067545138 hasConceptScore W2067545138C86803240 @default.
- W2067545138 hasIssue "46" @default.
- W2067545138 hasLocation W20675451381 @default.
- W2067545138 hasLocation W20675451382 @default.
- W2067545138 hasLocation W20675451383 @default.
- W2067545138 hasLocation W20675451384 @default.
- W2067545138 hasOpenAccess W2067545138 @default.
- W2067545138 hasPrimaryLocation W20675451381 @default.
- W2067545138 hasRelatedWork W1660052254 @default.
- W2067545138 hasRelatedWork W1996718803 @default.
- W2067545138 hasRelatedWork W2023260542 @default.
- W2067545138 hasRelatedWork W2026881606 @default.
- W2067545138 hasRelatedWork W2034577179 @default.
- W2067545138 hasRelatedWork W2050270212 @default.
- W2067545138 hasRelatedWork W2088412334 @default.
- W2067545138 hasRelatedWork W2123711162 @default.