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• W2067560668 abstract "RATIONALE: The mechanism for autoantibody production in systemic lupus erythematosus remains unclear. Dying cells are likely to be a source of autoantigens. We hypothesized that DCs may take up dying cells and induce autoimmunity.METHODS: Bone-marrow-derived DCs were co-cultured with syngeneic necrotic (freezing-thawing) or apoptotic (UV-irradiated) splenocytes. After 24 co-culture, DCs capturing dying cells were injected into female 11-week-old MRL/MP-+/+ mice. Blood was taken at two-week interval and was determined by ELISA for autoantibody level. Morphological changes in kidney were examined by immunofluorescent staining and transmission electron microscopy. T-cell stimulatory capacity of DCs was determined by mixed leukocyte reaction.RESULTS: Compared with DCs with apoptotic cells, DCs phagocytosing necrotic cells induced significantly higher serum levels of anti-double-stranded DNA antibodies IgG and IgG2a, (n = 3∼5, P<0.01), and higher level of proteinuria (P<0.05). Immunofluorescent staining showed strong immune complex deposition in kidney, which was further confirmed by transmission electron microscopy. Mice receiving DCs with necrotic cells developed subsequently a ‘butterfly’ facial skin lesion similar to that observed in human lupus. Flow cytometry analysis demonstrated that necrotic cells upregulated expression of surface MHC class II, CD80, CD86 and CD40 of DCs comparably to apoptotic cells. In response to lipopolysaccharide, DCs that have captured necrotic cells significantly secreted substantial TH1 cytokine INF-g at 24, 48, 72 hours (P<0.01). DCs that have phagocytosed necrotic cells significantly increased the proliferation of C57BL/6N and MRL/+ T cells (P<0.05).CONCLUSIONS: Activation of DCs by necrotic cells might promote TH1 autoimmune responses, leading to the systemic autoimmune disease in vivo. RATIONALE: The mechanism for autoantibody production in systemic lupus erythematosus remains unclear. Dying cells are likely to be a source of autoantigens. We hypothesized that DCs may take up dying cells and induce autoimmunity. METHODS: Bone-marrow-derived DCs were co-cultured with syngeneic necrotic (freezing-thawing) or apoptotic (UV-irradiated) splenocytes. After 24 co-culture, DCs capturing dying cells were injected into female 11-week-old MRL/MP-+/+ mice. Blood was taken at two-week interval and was determined by ELISA for autoantibody level. Morphological changes in kidney were examined by immunofluorescent staining and transmission electron microscopy. T-cell stimulatory capacity of DCs was determined by mixed leukocyte reaction. RESULTS: Compared with DCs with apoptotic cells, DCs phagocytosing necrotic cells induced significantly higher serum levels of anti-double-stranded DNA antibodies IgG and IgG2a, (n = 3∼5, P<0.01), and higher level of proteinuria (P<0.05). Immunofluorescent staining showed strong immune complex deposition in kidney, which was further confirmed by transmission electron microscopy. Mice receiving DCs with necrotic cells developed subsequently a ‘butterfly’ facial skin lesion similar to that observed in human lupus. Flow cytometry analysis demonstrated that necrotic cells upregulated expression of surface MHC class II, CD80, CD86 and CD40 of DCs comparably to apoptotic cells. In response to lipopolysaccharide, DCs that have captured necrotic cells significantly secreted substantial TH1 cytokine INF-g at 24, 48, 72 hours (P<0.01). DCs that have phagocytosed necrotic cells significantly increased the proliferation of C57BL/6N and MRL/+ T cells (P<0.05). CONCLUSIONS: Activation of DCs by necrotic cells might promote TH1 autoimmune responses, leading to the systemic autoimmune disease in vivo." @default.
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• W2067560668 date "2005-04-01" @default.
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• W2067560668 title "Necrotic Cells Induce Systemic Autoimmune Disease in vivo by Activation of Dendritic Cells (DCs)" @default.
• W2067560668 doi "https://doi.org/10.1016/j.jaci.2005.01.047" @default.
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