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- W2067582005 abstract "Studies of the kinetics of association and dissociation of the formycin nucleotides FTP and FDP with CF1 were carried out using the enhancement of formycin fluorescence. The protein used, derived from lettuce chloroplasts by chloroform induced release, contains only 4 types of subunit and has a molecular weight of 280 000. In the presence of 1.25 mM MgCl2, 1 mol of ATP or FTP is bound to the latent enzyme, with Kd = 10−7 or 2 · 10−7, respectively. The fluorescence emission (λmax 340 nm) of FTP is enhanced 3-fold upon binding, and polarization of fluorescence is markedly increased. The fluorescence changes have been used to follow FTP binding, which behaves as a bimolecular process with K1 = 2.4 · 104 M−1 · s−1. FTP is displaced by ATP in a process apparently involving unimolecular dissociation of FTP with k−1 = 3 · 10−3 s−1. The ratio of rates is comparable to the equilibrium constant and no additional steps have been observed. The protein has 3 sites for ADP binding. Rates of ADP binding are similar in magnitude to those for FTP. ADP and ATP sites are at least partly competitive with one another. The kinetics of nucleotide binding are strikingly altered upon activation of the protein as an ATPase. The rate of FTP binding increases to at least 106 M−1 · s−1. This suggests that activation involves lowering of the kinetic barriers to substrate and product binding-dissociation and has implications for the mechanism of energy transduction in photophosphorylation." @default.
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- W2067582005 date "1978-10-01" @default.
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- W2067582005 title "Kinetics of nucleotide binding to chloroplast coupling factor (CF1)" @default.
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- W2067582005 doi "https://doi.org/10.1016/0005-2728(78)90010-5" @default.
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