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- W2067609294 abstract "Sera from cattle vaccinated against either foot-and-mouth disease virus (FMDV) strains A10 Holland, 01 BFS, or C1 Detmold were tested in a serum neutralization test (SNT) and a liquid-phase blocking sandwich ELISA (LBE), and the titers were compared with the results of intradermolingual challenge tests. The LBE test results were significantly more reproducible (P < 0.005) than the SNT results. The correlation coefficients between SNT and LBE were 0.91 for FMDV strains A10 Holland and 01 BFS, and 0.82 for FMDV strain C1 Detmold (P < 0.0005). The regression coefficient for strain A10 Holland was 0.80, for strain 01 BFS the value was 0.87, and for strain C1 Detmold it was 0.64. In probit analysis, titers at which 95% of the cattle were protected against challenge with the homologous strain were determined for the SNT and the LBE. In the SNT the 95% protection levels for strains A10 Holland were ≥ 0.84, for O1 BFS ≥ 1.59, and for C1 Detmold ≥0.83. In the LBE they were ≥ 1.28, ≥ 1.71, and ≥ 1.74, respectively. Because the SNT and the LBE are highly significantly correlated, and the LBE is more reproducible, the LBE is likely to predict protection more reliably than the SNT." @default.
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- W2067609294 date "1989-11-01" @default.
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- W2067609294 title "Comparison of a liquid-phase blocking sandwich ELISA and a serum neutralization test to evaluate immunity in potency tests of foot-and-mouth disease vaccines" @default.
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- W2067609294 doi "https://doi.org/10.1016/0022-1759(89)90192-0" @default.
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