FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067612722> ?p ?o ?g. }

• W2067612722 endingPage "10733" @default.
• W2067612722 startingPage "10723" @default.
• W2067612722 abstract "A variety of bacterial pathogens use nanoscale protein fibers called type IV pili to mediate cell adhesion, a primary step leading to infection. Currently, how these nanofibers respond to mechanical stimuli and how this response is used to control adhesion is poorly understood. Here, we use atomic force microscopy techniques to quantify the forces guiding the adhesion of Pseudomonas aeruginosa type IV pili to surfaces. Using chemical force microscopy and single-cell force spectroscopy, we show that pili strongly bind to hydrophobic surfaces in a time-dependent manner, while they weakly bind to hydrophilic surfaces. Individual nanofibers are capable of withstanding forces up to 250 pN, thereby explaining how they can resist mechanical stress. Pulling on individual pili yields constant force plateaus, presumably reflecting conformational changes, as well as nanospring properties that may help bacteria to withstand physiological shear forces. Analysis of mutant strains demonstrates that these mechanical responses originate solely from type IV pili, while flagella and the cell surface localized and proposed pili-associated adhesin PilY1 play no direct role. We also demonstrate that bacterial–host interactions involve constant force plateaus, the extension of bacterial pili, and the formation of membrane tethers from host cells. We postulate that the unique mechanical responses of type IV pili unravelled here enable the bacteria to firmly attach to biotic and abiotic surfaces and thus maintain attachment when subjected to high shear forces under physiological conditions, helping to explain why pili play a critical role in colonization of the host." @default.
• W2067612722 created "2016-06-24" @default.
• W2067612722 creator A5009415203 @default.
• W2067612722 creator A5010700963 @default.
• W2067612722 creator A5015970740 @default.
• W2067612722 creator A5036077732 @default.
• W2067612722 creator A5047980423 @default.
• W2067612722 creator A5066628190 @default.
• W2067612722 date "2014-10-06" @default.
• W2067612722 modified "2023-10-11" @default.
• W2067612722 title "Nanoscale Adhesion Forces of <i>Pseudomonas aeruginosa</i> Type IV Pili" @default.
• W2067612722 cites W12119413 @default.
• W2067612722 cites W1502937395 @default.
• W2067612722 cites W1605211483 @default.
• W2067612722 cites W1628118805 @default.
• W2067612722 cites W1649510002 @default.
• W2067612722 cites W1732205261 @default.
• W2067612722 cites W1972628450 @default.
• W2067612722 cites W1973784950 @default.
• W2067612722 cites W1975383553 @default.
• W2067612722 cites W1999046178 @default.
• W2067612722 cites W2005237666 @default.
• W2067612722 cites W2007211819 @default.
• W2067612722 cites W2016158447 @default.
• W2067612722 cites W2016738734 @default.
• W2067612722 cites W2020719949 @default.
• W2067612722 cites W2031249988 @default.
• W2067612722 cites W2032643908 @default.
• W2067612722 cites W2038508954 @default.
• W2067612722 cites W2039817895 @default.
• W2067612722 cites W2044765734 @default.
• W2067612722 cites W2047785430 @default.
• W2067612722 cites W2056868286 @default.
• W2067612722 cites W2061814970 @default.
• W2067612722 cites W2061994684 @default.
• W2067612722 cites W2070344022 @default.
• W2067612722 cites W2079130094 @default.
• W2067612722 cites W2082730687 @default.
• W2067612722 cites W2083490229 @default.
• W2067612722 cites W2086987318 @default.
• W2067612722 cites W2089015231 @default.
• W2067612722 cites W2103515217 @default.
• W2067612722 cites W2110569167 @default.
• W2067612722 cites W2116533613 @default.
• W2067612722 cites W2122486748 @default.
• W2067612722 cites W2124704072 @default.
• W2067612722 cites W2128551110 @default.
• W2067612722 cites W2135689866 @default.
• W2067612722 cites W2144440398 @default.
• W2067612722 cites W2147165545 @default.
• W2067612722 cites W2147480596 @default.
• W2067612722 cites W2149886824 @default.
• W2067612722 cites W2163811015 @default.
• W2067612722 cites W2165044485 @default.
• W2067612722 doi "https://doi.org/10.1021/nn5044383" @default.
• W2067612722 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4212785" @default.
• W2067612722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25286300" @default.
• W2067612722 hasPublicationYear "2014" @default.
• W2067612722 type Work @default.
• W2067612722 sameAs 2067612722 @default.
• W2067612722 citedByCount "128" @default.
• W2067612722 countsByYear W20676127222014 @default.
• W2067612722 countsByYear W20676127222015 @default.
• W2067612722 countsByYear W20676127222016 @default.
• W2067612722 countsByYear W20676127222017 @default.
• W2067612722 countsByYear W20676127222018 @default.
• W2067612722 countsByYear W20676127222019 @default.
• W2067612722 countsByYear W20676127222020 @default.
• W2067612722 countsByYear W20676127222021 @default.
• W2067612722 countsByYear W20676127222022 @default.
• W2067612722 countsByYear W20676127222023 @default.
• W2067612722 crossrefType "journal-article" @default.
• W2067612722 hasAuthorship W2067612722A5009415203 @default.
• W2067612722 hasAuthorship W2067612722A5010700963 @default.
• W2067612722 hasAuthorship W2067612722A5015970740 @default.
• W2067612722 hasAuthorship W2067612722A5036077732 @default.
• W2067612722 hasAuthorship W2067612722A5047980423 @default.
• W2067612722 hasAuthorship W2067612722A5066628190 @default.
• W2067612722 hasBestOaLocation W20676127221 @default.
• W2067612722 hasConcept C102951782 @default.
• W2067612722 hasConcept C104317684 @default.
• W2067612722 hasConcept C119473381 @default.
• W2067612722 hasConcept C12554922 @default.
• W2067612722 hasConcept C171250308 @default.
• W2067612722 hasConcept C174832134 @default.
• W2067612722 hasConcept C178790620 @default.
• W2067612722 hasConcept C179470777 @default.
• W2067612722 hasConcept C185592680 @default.
• W2067612722 hasConcept C192562407 @default.
• W2067612722 hasConcept C2777637488 @default.
• W2067612722 hasConcept C39103817 @default.
• W2067612722 hasConcept C523546767 @default.
• W2067612722 hasConcept C54355233 @default.
• W2067612722 hasConcept C547475151 @default.
• W2067612722 hasConcept C55493867 @default.
• W2067612722 hasConcept C58123911 @default.
• W2067612722 hasConcept C84416704 @default.
• W2067612722 hasConcept C86803240 @default.

• next