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- W2067642978 abstract "A rapid spectrophotometric assay for the quantitation of prostaglandin synthetase activity has been devised. The principle of this method entails the measurement of the arachidonic acid-dependent formation of adrenochrome from L-epinephrine during prostaglandin (PG) biosynthesis. The validity of this assay was established by examining the substrate specificity of a variety of unsaturated fatty acids; their methyl esters, and the effect of inhibitors on PG synthetase. The results were in good agreement with those obtained by other assay methods. It was found that naphthalenediols, in particular 2,7-naphthalenediol, were potent inhibitors of prostaglandin synthetase at concentrations (I.D.50′ 2 × 10−6 M) comparable to indomethacin." @default.
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- W2067642978 date "1972-09-01" @default.
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- W2067642978 title "A rapid spectrophotometric assay for prostaglandin synthetase: Application to the study of non-steroidal antiinflammatory agents" @default.
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- W2067642978 doi "https://doi.org/10.1016/s0090-6980(72)80021-2" @default.
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