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- W2067682854 abstract "A quantitative assay for fluorescent heparin in a purified system and in plasma was developed (Piazolo et al: Semin Thromb Hemostas 20:227-235, 1994). The protamine microbeads (1.6 μm) showed a broad size distribution and a large standard variation in low concentrations. Our aim was to optimize these protamine microbeads for the measurement of fluorescent heparin. The following results were obtained: Paramagnetic protamine microbeads of different average diameters (0.8, 1.6, 2.8, and 4.5 μm) were synthesized by cyclocarbodiimide and tosyl activation. These microbeads bind heparin and are assayed using flow cytometry. The protamine concentration on the surface of the beads ranged between 2.0 and 61 mg/mL. The protamine microbeads bound fluorescent heparin and were analyzed by flow cytometry." @default.
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- W2067682854 date "1997-02-01" @default.
- W2067682854 modified "2023-09-26" @default.
- W2067682854 title "Measurement of Fluorescent-Labeled LMM-Heparin in Biological Fluids Using Protamine-Linked Microbeads" @default.
- W2067682854 doi "https://doi.org/10.1055/s-2007-996066" @default.
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