Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067687434> ?p ?o ?g. }
- W2067687434 endingPage "263" @default.
- W2067687434 startingPage "253" @default.
- W2067687434 abstract "The role of zinc ion in cytotoxicity following ischemic stroke, prolonged status epilepticus, and traumatic brain injury remains controversial, but likely is the result of mitochondrial dysfunction. We describe an excitation ratiometric fluorescence biosensor based on human carbonic anhydrase II variants expressed in the mitochondrial matrix, permitting free zinc levels to be quantitatively imaged therein. We observed an average mitochondrial matrix free zinc concentration of 0.2 pM in the PC12 rat pheochromacytoma cell culture line. Cytoplasmic and mitochondrial free zinc levels were imaged in a cellular oxygen glucose deprivation (OGD) model of ischemia/reperfusion. We observed a significant increase in mitochondrial zinc 1 h following 3 h OGD, at a time point when cytosolic zinc levels were depressed. Following the increase, mitochondrial zinc levels returned to physiological levels, while cytosolic zinc increased gradually over a 24 h time period in viable cells. The increase in intramitochondrial zinc observed during reoxygenation after OGD may contribute to bioenergetic dysfunction and cell death that occurs with both in vitro and in vivo models of reperfusion." @default.
- W2067687434 created "2016-06-24" @default.
- W2067687434 creator A5005302722 @default.
- W2067687434 creator A5030841413 @default.
- W2067687434 creator A5039035151 @default.
- W2067687434 creator A5053181915 @default.
- W2067687434 creator A5059279554 @default.
- W2067687434 creator A5062407037 @default.
- W2067687434 creator A5075383937 @default.
- W2067687434 creator A5080399968 @default.
- W2067687434 date "2012-03-20" @default.
- W2067687434 modified "2023-09-24" @default.
- W2067687434 title "Quantitative imaging of mitochondrial and cytosolic free zinc levels in an in vitro model of ischemia/reperfusion" @default.
- W2067687434 cites W1483020838 @default.
- W2067687434 cites W1531010890 @default.
- W2067687434 cites W1531700958 @default.
- W2067687434 cites W1587199968 @default.
- W2067687434 cites W1966613757 @default.
- W2067687434 cites W1968505653 @default.
- W2067687434 cites W1975312451 @default.
- W2067687434 cites W1988690043 @default.
- W2067687434 cites W1990697514 @default.
- W2067687434 cites W1996156357 @default.
- W2067687434 cites W2001612481 @default.
- W2067687434 cites W2002480100 @default.
- W2067687434 cites W2003570850 @default.
- W2067687434 cites W2008891372 @default.
- W2067687434 cites W2013986174 @default.
- W2067687434 cites W2023256273 @default.
- W2067687434 cites W2027532474 @default.
- W2067687434 cites W2027936807 @default.
- W2067687434 cites W2029107363 @default.
- W2067687434 cites W2031408496 @default.
- W2067687434 cites W2036839645 @default.
- W2067687434 cites W2050551315 @default.
- W2067687434 cites W2054566340 @default.
- W2067687434 cites W2056925277 @default.
- W2067687434 cites W2057520074 @default.
- W2067687434 cites W2058964568 @default.
- W2067687434 cites W2060465768 @default.
- W2067687434 cites W2062620729 @default.
- W2067687434 cites W2069400645 @default.
- W2067687434 cites W2069739744 @default.
- W2067687434 cites W2071213192 @default.
- W2067687434 cites W2072890593 @default.
- W2067687434 cites W2077449843 @default.
- W2067687434 cites W2077471914 @default.
- W2067687434 cites W2080160572 @default.
- W2067687434 cites W2080223471 @default.
- W2067687434 cites W2082469108 @default.
- W2067687434 cites W2087448538 @default.
- W2067687434 cites W2089287517 @default.
- W2067687434 cites W2092146469 @default.
- W2067687434 cites W2119492676 @default.
- W2067687434 cites W2129404156 @default.
- W2067687434 cites W2129426814 @default.
- W2067687434 cites W2131868763 @default.
- W2067687434 cites W2138149556 @default.
- W2067687434 cites W2144437860 @default.
- W2067687434 cites W2147849026 @default.
- W2067687434 cites W2149684302 @default.
- W2067687434 cites W2152887151 @default.
- W2067687434 cites W2162960825 @default.
- W2067687434 cites W2166439062 @default.
- W2067687434 cites W2168545543 @default.
- W2067687434 cites W2206752594 @default.
- W2067687434 cites W2331722871 @default.
- W2067687434 cites W4250998620 @default.
- W2067687434 cites W4252073970 @default.
- W2067687434 doi "https://doi.org/10.1007/s10863-012-9427-2" @default.
- W2067687434 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3539817" @default.
- W2067687434 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22430627" @default.
- W2067687434 hasPublicationYear "2012" @default.
- W2067687434 type Work @default.
- W2067687434 sameAs 2067687434 @default.
- W2067687434 citedByCount "55" @default.
- W2067687434 countsByYear W20676874342012 @default.
- W2067687434 countsByYear W20676874342013 @default.
- W2067687434 countsByYear W20676874342014 @default.
- W2067687434 countsByYear W20676874342015 @default.
- W2067687434 countsByYear W20676874342016 @default.
- W2067687434 countsByYear W20676874342017 @default.
- W2067687434 countsByYear W20676874342018 @default.
- W2067687434 countsByYear W20676874342019 @default.
- W2067687434 countsByYear W20676874342020 @default.
- W2067687434 countsByYear W20676874342021 @default.
- W2067687434 countsByYear W20676874342022 @default.
- W2067687434 countsByYear W20676874342023 @default.
- W2067687434 crossrefType "journal-article" @default.
- W2067687434 hasAuthorship W2067687434A5005302722 @default.
- W2067687434 hasAuthorship W2067687434A5030841413 @default.
- W2067687434 hasAuthorship W2067687434A5039035151 @default.
- W2067687434 hasAuthorship W2067687434A5053181915 @default.
- W2067687434 hasAuthorship W2067687434A5059279554 @default.
- W2067687434 hasAuthorship W2067687434A5062407037 @default.
- W2067687434 hasAuthorship W2067687434A5075383937 @default.
- W2067687434 hasAuthorship W2067687434A5080399968 @default.
- W2067687434 hasBestOaLocation W20676874342 @default.