FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067706619> ?p ?o ?g. }

• W2067706619 abstract "Monoclonal antibody TEC-02, raised against mouse embryonal carcinoma cells, has been shown to react with murine preimplantation embryos and with a very limited number of adult mouse tissues. The target epitope, TEC-2, is a carbohydrate carried in mouse embryonal carcinoma cells by large glycoprotein-bound glycan. We report here the expression of TEC-2 epitope on human carcinoma-derived cell lines, HeLa and HS, and the properties of its carbohydrate carriers. Immunolabeling of Nonidet P-40 lysates of HeLa cells separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that TEC-2 antigens are polydispersed glycoconjugates of high molecular weight (mostly above 100,000). TEC-2 antigens detected by the two-site sandwich assay, in which the antigen is immobilized and detected with the same antibody, had a slightly higher molecular weight than those detected by the solid-phase assay. This suggests heterogeneity in the number of TEC-2 epitopes per carrier molecule. When the cells were lysed by Triton X-114 and the detergent and aqueous phases were separated by warming and centrifugation, most of the TEC-2 antigenic activity was found in the aqueous phase. TEC-2 antigens isolated by indirect precipitation from [3H]galactose-labeled HeLa cells were degraded by extensive pronase digestion or mild alkaline treatment to glycopeptides or oligosaccharides of low molecular weight. Thus, TEC-2 epitope in human HeLa cells is carried by carbohydrates of only several monosaccharide units. TEC-02 antibody was also found to bind to Tamm-Horsfall glycoprotein isolated from human urine and its binding was enhanced by desialylation. Combined data indicate that TEC-02 antibody recognizes the GalNAc beta 1----4Gal beta 1----4 structure which may be carried on different types of molecule, according to the site of their synthesis." @default.
• W2067706619 created "2016-06-24" @default.
• W2067706619 creator A5018165739 @default.
• W2067706619 creator A5072717700 @default.
• W2067706619 creator A5076987605 @default.
• W2067706619 creator A5081464065 @default.
• W2067706619 creator A5085471620 @default.
• W2067706619 date "1988-03-01" @default.
• W2067706619 modified "2023-09-30" @default.
• W2067706619 title "Expression of mouse embryonic epitope TEC-2 on human carcinoma-derived cell lines and characterization of its glycoprotein carriers" @default.
• W2067706619 cites W1591208477 @default.
• W2067706619 cites W1949772429 @default.
• W2067706619 cites W1969884506 @default.
• W2067706619 cites W1973689359 @default.
• W2067706619 cites W1978079681 @default.
• W2067706619 cites W1989989027 @default.
• W2067706619 cites W2000215955 @default.
• W2067706619 cites W2001743581 @default.
• W2067706619 cites W2009857031 @default.
• W2067706619 cites W2019663874 @default.
• W2067706619 cites W2021159463 @default.
• W2067706619 cites W2022797612 @default.
• W2067706619 cites W2031901918 @default.
• W2067706619 cites W2033080437 @default.
• W2067706619 cites W2033447452 @default.
• W2067706619 cites W2044527577 @default.
• W2067706619 cites W2049560605 @default.
• W2067706619 cites W2050586036 @default.
• W2067706619 cites W2067546678 @default.
• W2067706619 cites W2070487895 @default.
• W2067706619 cites W2083516187 @default.
• W2067706619 cites W2095463924 @default.
• W2067706619 cites W2106448082 @default.
• W2067706619 cites W2112584361 @default.
• W2067706619 cites W2113228804 @default.
• W2067706619 cites W2205599207 @default.
• W2067706619 cites W2408300131 @default.
• W2067706619 cites W3213082070 @default.
• W2067706619 cites W4239139991 @default.
• W2067706619 doi "https://doi.org/10.1016/0167-4889(88)90020-1" @default.
• W2067706619 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2449911" @default.
• W2067706619 hasPublicationYear "1988" @default.
• W2067706619 type Work @default.
• W2067706619 sameAs 2067706619 @default.
• W2067706619 citedByCount "4" @default.
• W2067706619 crossrefType "journal-article" @default.
• W2067706619 hasAuthorship W2067706619A5018165739 @default.
• W2067706619 hasAuthorship W2067706619A5072717700 @default.
• W2067706619 hasAuthorship W2067706619A5076987605 @default.
• W2067706619 hasAuthorship W2067706619A5081464065 @default.
• W2067706619 hasAuthorship W2067706619A5085471620 @default.
• W2067706619 hasConcept C108625454 @default.
• W2067706619 hasConcept C13426554 @default.
• W2067706619 hasConcept C147483822 @default.
• W2067706619 hasConcept C153911025 @default.
• W2067706619 hasConcept C159654299 @default.
• W2067706619 hasConcept C185592680 @default.
• W2067706619 hasConcept C195616568 @default.
• W2067706619 hasConcept C203014093 @default.
• W2067706619 hasConcept C2780115458 @default.
• W2067706619 hasConcept C542903549 @default.
• W2067706619 hasConcept C55493867 @default.
• W2067706619 hasConcept C86803240 @default.
• W2067706619 hasConceptScore W2067706619C108625454 @default.
• W2067706619 hasConceptScore W2067706619C13426554 @default.
• W2067706619 hasConceptScore W2067706619C147483822 @default.
• W2067706619 hasConceptScore W2067706619C153911025 @default.
• W2067706619 hasConceptScore W2067706619C159654299 @default.
• W2067706619 hasConceptScore W2067706619C185592680 @default.
• W2067706619 hasConceptScore W2067706619C195616568 @default.
• W2067706619 hasConceptScore W2067706619C203014093 @default.
• W2067706619 hasConceptScore W2067706619C2780115458 @default.
• W2067706619 hasConceptScore W2067706619C542903549 @default.
• W2067706619 hasConceptScore W2067706619C55493867 @default.
• W2067706619 hasConceptScore W2067706619C86803240 @default.
• W2067706619 hasLocation W20677066191 @default.
• W2067706619 hasLocation W20677066192 @default.
• W2067706619 hasOpenAccess W2067706619 @default.
• W2067706619 hasPrimaryLocation W20677066191 @default.
• W2067706619 hasRelatedWork W1993056856 @default.
• W2067706619 hasRelatedWork W2005442970 @default.
• W2067706619 hasRelatedWork W2009897990 @default.
• W2067706619 hasRelatedWork W2019523897 @default.
• W2067706619 hasRelatedWork W2085942867 @default.
• W2067706619 hasRelatedWork W2271391931 @default.
• W2067706619 hasRelatedWork W2396268617 @default.
• W2067706619 hasRelatedWork W2418014537 @default.
• W2067706619 hasRelatedWork W2465371110 @default.
• W2067706619 hasRelatedWork W63000111 @default.
• W2067706619 isParatext "false" @default.
• W2067706619 isRetracted "false" @default.
• W2067706619 magId "2067706619" @default.
• W2067706619 workType "article" @default.