FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067795874> ?p ?o ?g. }

• W2067795874 endingPage "1244" @default.
• W2067795874 startingPage "1235" @default.
• W2067795874 abstract "The feasibility of ex vivo blood production is limited by both biological and engineering challenges. From an engineering perspective, these challenges include the significant volumes required to generate even a single unit of a blood product, as well as the correspondingly high protein consumption required for such large volume cultures. Membrane bioreactors, such as hollow fiber bioreactors (HFBRs), enable cell densities approximately 100-fold greater than traditional culture systems and therefore may enable a significant reduction in culture working volumes. As cultured cells, and larger molecules, are retained within a fraction of the system volume, via a semipermeable membrane it may be possible to reduce protein consumption by limiting supplementation to only this fraction. Typically, HFBRs are complex perfusion systems having total volumes incompatible with bench scale screening and optimization of stem cell-based cultures. In this article we describe the use of a simplified HFBR system to assess the feasibility of this technology to produce blood products from umbilical cord blood-derived CD34 + hematopoietic stem progenitor cells (HSPCs). Unlike conventional HFBR systems used for protein manufacture, where cells are cultured in the extracapillary space, we have cultured cells in the intracapillary space, which is likely more compatible with the large-scale production of blood cell suspension cultures. Using this platform we direct HSPCs down the myeloid lineage, while targeting a 100-fold increase in cell density and the use of protein-free bulk medium. Our results demonstrate the potential of this system to deliver high cell densities, even in the absence of protein supplementation of the bulk medium." @default.
• W2067795874 created "2016-06-24" @default.
• W2067795874 creator A5010808182 @default.
• W2067795874 creator A5043169076 @default.
• W2067795874 creator A5046535725 @default.
• W2067795874 creator A5052018750 @default.
• W2067795874 creator A5061172047 @default.
• W2067795874 creator A5077128701 @default.
• W2067795874 date "2012-06-01" @default.
• W2067795874 modified "2023-10-18" @default.
• W2067795874 title "Bioreactor for Blood Product Production" @default.
• W2067795874 cites W1553660045 @default.
• W2067795874 cites W1969170451 @default.
• W2067795874 cites W1971018369 @default.
• W2067795874 cites W1973020021 @default.
• W2067795874 cites W1984675553 @default.
• W2067795874 cites W1986316867 @default.
• W2067795874 cites W1990185169 @default.
• W2067795874 cites W2019765563 @default.
• W2067795874 cites W2028860972 @default.
• W2067795874 cites W2040237046 @default.
• W2067795874 cites W2044757085 @default.
• W2067795874 cites W2045459881 @default.
• W2067795874 cites W2047215805 @default.
• W2067795874 cites W2062254735 @default.
• W2067795874 cites W2066655819 @default.
• W2067795874 cites W2070017145 @default.
• W2067795874 cites W2079425508 @default.
• W2067795874 cites W2083017100 @default.
• W2067795874 cites W2105372361 @default.
• W2067795874 cites W2110956170 @default.
• W2067795874 cites W2121377840 @default.
• W2067795874 cites W2163634077 @default.
• W2067795874 cites W2165031167 @default.
• W2067795874 cites W2215306508 @default.
• W2067795874 doi "https://doi.org/10.3727/096368911x627363" @default.
• W2067795874 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22405378" @default.
• W2067795874 hasPublicationYear "2012" @default.
• W2067795874 type Work @default.
• W2067795874 sameAs 2067795874 @default.
• W2067795874 citedByCount "6" @default.
• W2067795874 countsByYear W20677958742014 @default.
• W2067795874 countsByYear W20677958742015 @default.
• W2067795874 countsByYear W20677958742019 @default.
• W2067795874 countsByYear W20677958742021 @default.
• W2067795874 crossrefType "journal-article" @default.
• W2067795874 hasAuthorship W2067795874A5010808182 @default.
• W2067795874 hasAuthorship W2067795874A5043169076 @default.
• W2067795874 hasAuthorship W2067795874A5046535725 @default.
• W2067795874 hasAuthorship W2067795874A5052018750 @default.
• W2067795874 hasAuthorship W2067795874A5061172047 @default.
• W2067795874 hasAuthorship W2067795874A5077128701 @default.
• W2067795874 hasBestOaLocation W20677958742 @default.
• W2067795874 hasConcept C10205521 @default.
• W2067795874 hasConcept C109159458 @default.
• W2067795874 hasConcept C136229726 @default.
• W2067795874 hasConcept C168170006 @default.
• W2067795874 hasConcept C178790620 @default.
• W2067795874 hasConcept C185592680 @default.
• W2067795874 hasConcept C201750760 @default.
• W2067795874 hasConcept C28328180 @default.
• W2067795874 hasConcept C54355233 @default.
• W2067795874 hasConcept C71924100 @default.
• W2067795874 hasConcept C81885089 @default.
• W2067795874 hasConcept C86803240 @default.
• W2067795874 hasConcept C95444343 @default.
• W2067795874 hasConceptScore W2067795874C10205521 @default.
• W2067795874 hasConceptScore W2067795874C109159458 @default.
• W2067795874 hasConceptScore W2067795874C136229726 @default.
• W2067795874 hasConceptScore W2067795874C168170006 @default.
• W2067795874 hasConceptScore W2067795874C178790620 @default.
• W2067795874 hasConceptScore W2067795874C185592680 @default.
• W2067795874 hasConceptScore W2067795874C201750760 @default.
• W2067795874 hasConceptScore W2067795874C28328180 @default.
• W2067795874 hasConceptScore W2067795874C54355233 @default.
• W2067795874 hasConceptScore W2067795874C71924100 @default.
• W2067795874 hasConceptScore W2067795874C81885089 @default.
• W2067795874 hasConceptScore W2067795874C86803240 @default.
• W2067795874 hasConceptScore W2067795874C95444343 @default.
• W2067795874 hasIssue "6" @default.
• W2067795874 hasLocation W20677958741 @default.
• W2067795874 hasLocation W20677958742 @default.
• W2067795874 hasLocation W20677958743 @default.
• W2067795874 hasOpenAccess W2067795874 @default.
• W2067795874 hasPrimaryLocation W20677958741 @default.
• W2067795874 hasRelatedWork W1812483684 @default.
• W2067795874 hasRelatedWork W1998228373 @default.
• W2067795874 hasRelatedWork W2044559595 @default.
• W2067795874 hasRelatedWork W2054167115 @default.
• W2067795874 hasRelatedWork W2067975567 @default.
• W2067795874 hasRelatedWork W2097466377 @default.
• W2067795874 hasRelatedWork W2156396639 @default.
• W2067795874 hasRelatedWork W2518614969 @default.
• W2067795874 hasRelatedWork W4376111324 @default.
• W2067795874 hasRelatedWork W4376112277 @default.
• W2067795874 hasVolume "21" @default.
• W2067795874 isParatext "false" @default.
• W2067795874 isRetracted "false" @default.

• next