Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067854319> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2067854319 endingPage "1202" @default.
- W2067854319 startingPage "1196" @default.
- W2067854319 abstract "The noncovalent binding of the near-infrared (NIR) dyes, DTTCI (cationic) and IR-125 (anionic), to several model proteins was investigated with the use of steady-state and picosecond laser fluorescence measurements. In an aqueous borate buffer (pH = 9.2), minimal fluorescence emission from these NIR dyes was observed. When a protein was added to the solution, enhancements in the fluorescence emission were found for both dyes. Time-resolved fluorescence measurements for IR-125 in the presence of the protein, β-casein, indicated a biexponential decay with lifetimes of 195 and 682 ps (χ 2 = 1.94). Our data suggest that these dyes distribute themselves between the hydrophobic core of the protein and the interstitial aqueous solution. The dye molecules residing in the interior of the protein exhibit enhancements in their fluorescence due to a more favorable microenvironment. The binding and enhanced fluorescence properties allowed the use of these dyes as noncovalent stains for the low-level detection of proteins separated via capillary electrophoresis (CE). Detection limits for some model proteins separated by CE and stained with these NIR dyes were found to be superior to those obtained by using UV detection in CE." @default.
- W2067854319 created "2016-06-24" @default.
- W2067854319 creator A5001278959 @default.
- W2067854319 creator A5038208939 @default.
- W2067854319 date "1996-09-01" @default.
- W2067854319 modified "2023-10-17" @default.
- W2067854319 title "Binding Properties of Near-IR Dyes to Proteins and Separation of the Dye/Protein Complexes Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection" @default.
- W2067854319 cites W1926033671 @default.
- W2067854319 cites W1963854720 @default.
- W2067854319 cites W1965843819 @default.
- W2067854319 cites W1967381821 @default.
- W2067854319 cites W1970286599 @default.
- W2067854319 cites W1980036883 @default.
- W2067854319 cites W1980094690 @default.
- W2067854319 cites W1980436729 @default.
- W2067854319 cites W1985529268 @default.
- W2067854319 cites W1985649733 @default.
- W2067854319 cites W1990481684 @default.
- W2067854319 cites W1996215483 @default.
- W2067854319 cites W1996261030 @default.
- W2067854319 cites W1999173093 @default.
- W2067854319 cites W2015866871 @default.
- W2067854319 cites W2023869821 @default.
- W2067854319 cites W2025870130 @default.
- W2067854319 cites W2031281208 @default.
- W2067854319 cites W2036704425 @default.
- W2067854319 cites W2041235392 @default.
- W2067854319 cites W2044278795 @default.
- W2067854319 cites W2047216280 @default.
- W2067854319 cites W2047231243 @default.
- W2067854319 cites W2050028302 @default.
- W2067854319 cites W2052191535 @default.
- W2067854319 cites W2059443069 @default.
- W2067854319 cites W2064862042 @default.
- W2067854319 cites W2079138391 @default.
- W2067854319 cites W2086348627 @default.
- W2067854319 cites W2160502870 @default.
- W2067854319 doi "https://doi.org/10.1366/0003702963905079" @default.
- W2067854319 hasPublicationYear "1996" @default.
- W2067854319 type Work @default.
- W2067854319 sameAs 2067854319 @default.
- W2067854319 citedByCount "29" @default.
- W2067854319 countsByYear W20678543192012 @default.
- W2067854319 countsByYear W20678543192014 @default.
- W2067854319 crossrefType "journal-article" @default.
- W2067854319 hasAuthorship W2067854319A5001278959 @default.
- W2067854319 hasAuthorship W2067854319A5038208939 @default.
- W2067854319 hasConcept C113196181 @default.
- W2067854319 hasConcept C121332964 @default.
- W2067854319 hasConcept C1342733 @default.
- W2067854319 hasConcept C158711907 @default.
- W2067854319 hasConcept C178790620 @default.
- W2067854319 hasConcept C183882617 @default.
- W2067854319 hasConcept C184651966 @default.
- W2067854319 hasConcept C185592680 @default.
- W2067854319 hasConcept C2900643 @default.
- W2067854319 hasConcept C40250595 @default.
- W2067854319 hasConcept C43617362 @default.
- W2067854319 hasConcept C62520636 @default.
- W2067854319 hasConcept C75473681 @default.
- W2067854319 hasConcept C91881484 @default.
- W2067854319 hasConceptScore W2067854319C113196181 @default.
- W2067854319 hasConceptScore W2067854319C121332964 @default.
- W2067854319 hasConceptScore W2067854319C1342733 @default.
- W2067854319 hasConceptScore W2067854319C158711907 @default.
- W2067854319 hasConceptScore W2067854319C178790620 @default.
- W2067854319 hasConceptScore W2067854319C183882617 @default.
- W2067854319 hasConceptScore W2067854319C184651966 @default.
- W2067854319 hasConceptScore W2067854319C185592680 @default.
- W2067854319 hasConceptScore W2067854319C2900643 @default.
- W2067854319 hasConceptScore W2067854319C40250595 @default.
- W2067854319 hasConceptScore W2067854319C43617362 @default.
- W2067854319 hasConceptScore W2067854319C62520636 @default.
- W2067854319 hasConceptScore W2067854319C75473681 @default.
- W2067854319 hasConceptScore W2067854319C91881484 @default.
- W2067854319 hasIssue "9" @default.
- W2067854319 hasLocation W20678543191 @default.
- W2067854319 hasOpenAccess W2067854319 @default.
- W2067854319 hasPrimaryLocation W20678543191 @default.
- W2067854319 hasRelatedWork W182026734 @default.
- W2067854319 hasRelatedWork W1995054819 @default.
- W2067854319 hasRelatedWork W2047597498 @default.
- W2067854319 hasRelatedWork W2062719312 @default.
- W2067854319 hasRelatedWork W2078000306 @default.
- W2067854319 hasRelatedWork W2080501511 @default.
- W2067854319 hasRelatedWork W2137066617 @default.
- W2067854319 hasRelatedWork W2796296058 @default.
- W2067854319 hasRelatedWork W4245894379 @default.
- W2067854319 hasRelatedWork W2106966118 @default.
- W2067854319 hasVolume "50" @default.
- W2067854319 isParatext "false" @default.
- W2067854319 isRetracted "false" @default.
- W2067854319 magId "2067854319" @default.
- W2067854319 workType "article" @default.