Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067856724> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2067856724 endingPage "914" @default.
- W2067856724 startingPage "905" @default.
- W2067856724 abstract "Binding sites for concanvalin A (Con A), Ricinus communis I agglutinin (RCA I), and Helix pomatia lectin (HPA) were localized in the Golgi apparatus of rat small intestinal absorptive cells. A preembedment technique, a modification of the one originally used by Bernhard and Avrameas (Exp Cell Res 64:232, 1971), was employed, with horse-radish peroxidase being used for cytochemical visualization. Incubations were performed on 10 microns thick cryosections of duodenal segments that were fixed in a mixture of 4% formaldehyde and 0.5% glutaraldehyde; fixation was preceded by a 2-min rinse in 0.1 M sodium cacodylate and followed by storage in the same buffer for up to 7 days. Incubation with Con A, which binds preferably to alpha-D-mannose and alpha-D-glucose residues, caused intense reaction of the dilated cisternae of the cis Golgi side; staining was variable in intermediate and trans cisternae. RCA I, recognizing beta-D-galactose residues, could only be demonstrated in intermediate cisternae. Reaction for HPA, which indicates alpha-N-acetyl-D-galactosamine residues, stained intensely 1 to 2 cisternae of the cis Golgi side, as well as being localized in the peripheral regions of the cisternae of the intermediate compartment of the stacks. Deposits of reaction product covered the luminal surface of the cisternal membranes, but usually left the lumen itself, as well as lipid particles, devoid of reaction. The differences in Con A, RCA I, and HPA reactivity between cis, intermediate, and trans cisternae suggest compositional and structural differences of the carbohydrates in the respective compartments; they may reflect conversion processes that are known to occur in the oligosaccharide side chains of glycoconjugates at the Golgi complex level." @default.
- W2067856724 created "2016-06-24" @default.
- W2067856724 creator A5014396537 @default.
- W2067856724 creator A5055695661 @default.
- W2067856724 date "1985-09-01" @default.
- W2067856724 modified "2023-10-16" @default.
- W2067856724 title "Localization of binding sites for concanavalin A, Ricinus communis I and Helix pomatia lectin in the Golgi apparatus of rat small intestinal absorptive cells." @default.
- W2067856724 doi "https://doi.org/10.1177/33.9.4020101" @default.
- W2067856724 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4020101" @default.
- W2067856724 hasPublicationYear "1985" @default.
- W2067856724 type Work @default.
- W2067856724 sameAs 2067856724 @default.
- W2067856724 citedByCount "35" @default.
- W2067856724 countsByYear W20678567242012 @default.
- W2067856724 countsByYear W20678567242013 @default.
- W2067856724 countsByYear W20678567242017 @default.
- W2067856724 countsByYear W20678567242018 @default.
- W2067856724 countsByYear W20678567242019 @default.
- W2067856724 crossrefType "journal-article" @default.
- W2067856724 hasAuthorship W2067856724A5014396537 @default.
- W2067856724 hasAuthorship W2067856724A5055695661 @default.
- W2067856724 hasBestOaLocation W20678567241 @default.
- W2067856724 hasConcept C119062480 @default.
- W2067856724 hasConcept C12554922 @default.
- W2067856724 hasConcept C158617107 @default.
- W2067856724 hasConcept C185592680 @default.
- W2067856724 hasConcept C18903297 @default.
- W2067856724 hasConcept C19869320 @default.
- W2067856724 hasConcept C202751555 @default.
- W2067856724 hasConcept C2777127260 @default.
- W2067856724 hasConcept C2777529624 @default.
- W2067856724 hasConcept C2777959311 @default.
- W2067856724 hasConcept C2779965526 @default.
- W2067856724 hasConcept C2780758401 @default.
- W2067856724 hasConcept C43617362 @default.
- W2067856724 hasConcept C55493867 @default.
- W2067856724 hasConcept C86803240 @default.
- W2067856724 hasConceptScore W2067856724C119062480 @default.
- W2067856724 hasConceptScore W2067856724C12554922 @default.
- W2067856724 hasConceptScore W2067856724C158617107 @default.
- W2067856724 hasConceptScore W2067856724C185592680 @default.
- W2067856724 hasConceptScore W2067856724C18903297 @default.
- W2067856724 hasConceptScore W2067856724C19869320 @default.
- W2067856724 hasConceptScore W2067856724C202751555 @default.
- W2067856724 hasConceptScore W2067856724C2777127260 @default.
- W2067856724 hasConceptScore W2067856724C2777529624 @default.
- W2067856724 hasConceptScore W2067856724C2777959311 @default.
- W2067856724 hasConceptScore W2067856724C2779965526 @default.
- W2067856724 hasConceptScore W2067856724C2780758401 @default.
- W2067856724 hasConceptScore W2067856724C43617362 @default.
- W2067856724 hasConceptScore W2067856724C55493867 @default.
- W2067856724 hasConceptScore W2067856724C86803240 @default.
- W2067856724 hasIssue "9" @default.
- W2067856724 hasLocation W20678567241 @default.
- W2067856724 hasLocation W20678567242 @default.
- W2067856724 hasOpenAccess W2067856724 @default.
- W2067856724 hasPrimaryLocation W20678567241 @default.
- W2067856724 hasRelatedWork W1571455279 @default.
- W2067856724 hasRelatedWork W1603770979 @default.
- W2067856724 hasRelatedWork W2003067913 @default.
- W2067856724 hasRelatedWork W2034207274 @default.
- W2067856724 hasRelatedWork W2067856724 @default.
- W2067856724 hasRelatedWork W2313516846 @default.
- W2067856724 hasRelatedWork W2322439629 @default.
- W2067856724 hasRelatedWork W2472557834 @default.
- W2067856724 hasRelatedWork W2606946045 @default.
- W2067856724 hasRelatedWork W4289992636 @default.
- W2067856724 hasVolume "33" @default.
- W2067856724 isParatext "false" @default.
- W2067856724 isRetracted "false" @default.
- W2067856724 magId "2067856724" @default.
- W2067856724 workType "article" @default.