FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067860976> ?p ?o ?g. }

• W2067860976 endingPage "878" @default.
• W2067860976 startingPage "873" @default.
• W2067860976 abstract "Maize (Zea mays) protoporphyrinogen IX oxidase (PPO: EC 1.3.3.4) possesses a chloroplast transit peptide (CTP) that delivers the enzyme into the chloroplast. The cleavage site yielding the mature protein was predicted by using the ChloroP software and by comparing conserved regions of the available plant PPO sequences. In parallel, the processed NH(2)-terminus of native PPO was identified experimentally by microsequencing the immunoprecipitated plant PPO from maize etioplasts. The cleavage sites identified using the bioinformatic approaches did not match the experimental result. The three sequences have been cloned and expressed in bacteria and their kinetics were compared in order to understand if the generated proteins had biochemically relevant differences. Recombinant PPO corresponding to the native PPO accumulated at higher level and was more active than the two homologues. A cysteine present in the CTP seems to be able to modify the redox state of the enzyme and to be responsible for the alteration of the kinetic features. In contrast, the sensitivity to different herbicides was unaffected by modifications at the NH(2)-terminus, suggesting that the mode of action is non-competitive and that the NH(2)-terminus is involved in the recognition of the natural substrate." @default.
• W2067860976 created "2016-06-24" @default.
• W2067860976 creator A5022285521 @default.
• W2067860976 creator A5051177481 @default.
• W2067860976 creator A5072057151 @default.
• W2067860976 creator A5078271307 @default.
• W2067860976 date "2003-10-01" @default.
• W2067860976 modified "2023-10-09" @default.
• W2067860976 title "Correct identification of the chloroplastic protoporphyrinogen IX oxidase N-terminus places the biochemical data in frame" @default.
• W2067860976 cites W1485269876 @default.
• W2067860976 cites W1511978288 @default.
• W2067860976 cites W1568006607 @default.
• W2067860976 cites W1965045785 @default.
• W2067860976 cites W1968735747 @default.
• W2067860976 cites W1971071241 @default.
• W2067860976 cites W1973106501 @default.
• W2067860976 cites W1989033034 @default.
• W2067860976 cites W1995749871 @default.
• W2067860976 cites W2016582379 @default.
• W2067860976 cites W2019309665 @default.
• W2067860976 cites W2025221775 @default.
• W2067860976 cites W2026493864 @default.
• W2067860976 cites W2028858718 @default.
• W2067860976 cites W2029684104 @default.
• W2067860976 cites W2030566353 @default.
• W2067860976 cites W2037368454 @default.
• W2067860976 cites W2040380764 @default.
• W2067860976 cites W2054441789 @default.
• W2067860976 cites W2060303895 @default.
• W2067860976 cites W2068861782 @default.
• W2067860976 cites W2091509777 @default.
• W2067860976 cites W2100837269 @default.
• W2067860976 cites W2107085610 @default.
• W2067860976 cites W2111330501 @default.
• W2067860976 cites W2129095580 @default.
• W2067860976 cites W2132231478 @default.
• W2067860976 cites W2159410139 @default.
• W2067860976 cites W2171091522 @default.
• W2067860976 cites W2275008338 @default.
• W2067860976 cites W2331207181 @default.
• W2067860976 cites W2417161131 @default.
• W2067860976 cites W592059757 @default.
• W2067860976 doi "https://doi.org/10.1016/j.bbrc.2003.08.079" @default.
• W2067860976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/13679054" @default.
• W2067860976 hasPublicationYear "2003" @default.
• W2067860976 type Work @default.
• W2067860976 sameAs 2067860976 @default.
• W2067860976 citedByCount "4" @default.
• W2067860976 countsByYear W20678609762017 @default.
• W2067860976 countsByYear W20678609762021 @default.
• W2067860976 crossrefType "journal-article" @default.
• W2067860976 hasAuthorship W2067860976A5022285521 @default.
• W2067860976 hasAuthorship W2067860976A5051177481 @default.
• W2067860976 hasAuthorship W2067860976A5072057151 @default.
• W2067860976 hasAuthorship W2067860976A5078271307 @default.
• W2067860976 hasConcept C104317684 @default.
• W2067860976 hasConcept C121436942 @default.
• W2067860976 hasConcept C14053116 @default.
• W2067860976 hasConcept C151730666 @default.
• W2067860976 hasConcept C167625842 @default.
• W2067860976 hasConcept C175156509 @default.
• W2067860976 hasConcept C181199279 @default.
• W2067860976 hasConcept C185592680 @default.
• W2067860976 hasConcept C2779201268 @default.
• W2067860976 hasConcept C2779281246 @default.
• W2067860976 hasConcept C2909256915 @default.
• W2067860976 hasConcept C43369102 @default.
• W2067860976 hasConcept C55493867 @default.
• W2067860976 hasConcept C69305403 @default.
• W2067860976 hasConcept C86803240 @default.
• W2067860976 hasConcept C93829228 @default.
• W2067860976 hasConceptScore W2067860976C104317684 @default.
• W2067860976 hasConceptScore W2067860976C121436942 @default.
• W2067860976 hasConceptScore W2067860976C14053116 @default.
• W2067860976 hasConceptScore W2067860976C151730666 @default.
• W2067860976 hasConceptScore W2067860976C167625842 @default.
• W2067860976 hasConceptScore W2067860976C175156509 @default.
• W2067860976 hasConceptScore W2067860976C181199279 @default.
• W2067860976 hasConceptScore W2067860976C185592680 @default.
• W2067860976 hasConceptScore W2067860976C2779201268 @default.
• W2067860976 hasConceptScore W2067860976C2779281246 @default.
• W2067860976 hasConceptScore W2067860976C2909256915 @default.
• W2067860976 hasConceptScore W2067860976C43369102 @default.
• W2067860976 hasConceptScore W2067860976C55493867 @default.
• W2067860976 hasConceptScore W2067860976C69305403 @default.
• W2067860976 hasConceptScore W2067860976C86803240 @default.
• W2067860976 hasConceptScore W2067860976C93829228 @default.
• W2067860976 hasIssue "4" @default.
• W2067860976 hasLocation W20678609761 @default.
• W2067860976 hasLocation W20678609762 @default.
• W2067860976 hasOpenAccess W2067860976 @default.
• W2067860976 hasPrimaryLocation W20678609761 @default.
• W2067860976 hasRelatedWork W1988989271 @default.
• W2067860976 hasRelatedWork W2043410894 @default.
• W2067860976 hasRelatedWork W2063946984 @default.
• W2067860976 hasRelatedWork W2067860976 @default.
• W2067860976 hasRelatedWork W2142260297 @default.
• W2067860976 hasRelatedWork W223485786 @default.

• next