Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067863352> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2067863352 endingPage "4482" @default.
- W2067863352 startingPage "4478" @default.
- W2067863352 abstract "A shortened form of the Tetrahymena intervening sequence (IVS) RNA acts as an enzyme, catalyzing nucleotidyl transfer and hydrolysis reactions with oligo(cytidylic acid) substrates [Zaug, A. J., & Cech, T. R. (1986) Science (Washington, D.C.) 231, 470-475]. These reactions involve phosphodiester substrates. We now show that the same enzyme has activity toward phosphate monoesters. The 3'-phosphate of C5p or C6p is transferred to the 3'-terminal guanosine of the enzyme. The pH dependence of the reaction (optimum at pH 5) indicates that the enzyme has activity toward the dianion and much greater activity toward the monoanion form of the 3'-phosphate of the substrate. Phosphorylation of the enzyme is reversible by C5-OH and other oligo(pyrimidines) such as UCU-OH. Thus, the RNA enzyme acts as a phosphotransferase, transferring the 3'-terminal phosphate of C5p to UCU-OH with multiple turnover. At pH 4 and 5, the phosphoenzyme undergoes slow hydrolysis to yield inorganic phosphate. Thus, the enzyme has acid phosphatase activity. The RNA enzyme dephosphorylates oligonucleotide substrates with high sequence specificity, which distinguishes it from known protein enzymes." @default.
- W2067863352 created "2016-06-24" @default.
- W2067863352 creator A5017573996 @default.
- W2067863352 creator A5090896407 @default.
- W2067863352 date "1986-08-01" @default.
- W2067863352 modified "2023-09-25" @default.
- W2067863352 title "The Tetrahymena intervening sequence ribonucleic acid enzyme is a phosphotransferase and an acid phosphatase" @default.
- W2067863352 cites W1603760485 @default.
- W2067863352 cites W1964915470 @default.
- W2067863352 cites W1969292324 @default.
- W2067863352 cites W1978847377 @default.
- W2067863352 cites W1979496015 @default.
- W2067863352 cites W1984859924 @default.
- W2067863352 cites W1989200332 @default.
- W2067863352 cites W1991984236 @default.
- W2067863352 cites W2009708970 @default.
- W2067863352 cites W2017139765 @default.
- W2067863352 cites W2020677628 @default.
- W2067863352 cites W2020895445 @default.
- W2067863352 cites W2047865110 @default.
- W2067863352 cites W2056235466 @default.
- W2067863352 cites W2069115217 @default.
- W2067863352 cites W2081245595 @default.
- W2067863352 cites W2087732491 @default.
- W2067863352 cites W2153630542 @default.
- W2067863352 cites W2154989771 @default.
- W2067863352 cites W2331377560 @default.
- W2067863352 cites W88565723 @default.
- W2067863352 cites W2166824799 @default.
- W2067863352 doi "https://doi.org/10.1021/bi00364a002" @default.
- W2067863352 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2429688" @default.
- W2067863352 hasPublicationYear "1986" @default.
- W2067863352 type Work @default.
- W2067863352 sameAs 2067863352 @default.
- W2067863352 citedByCount "49" @default.
- W2067863352 countsByYear W20678633522013 @default.
- W2067863352 countsByYear W20678633522014 @default.
- W2067863352 countsByYear W20678633522020 @default.
- W2067863352 countsByYear W20678633522021 @default.
- W2067863352 countsByYear W20678633522022 @default.
- W2067863352 countsByYear W20678633522023 @default.
- W2067863352 crossrefType "journal-article" @default.
- W2067863352 hasAuthorship W2067863352A5017573996 @default.
- W2067863352 hasAuthorship W2067863352A5090896407 @default.
- W2067863352 hasConcept C104317684 @default.
- W2067863352 hasConcept C178666793 @default.
- W2067863352 hasConcept C181199279 @default.
- W2067863352 hasConcept C185592680 @default.
- W2067863352 hasConcept C18903297 @default.
- W2067863352 hasConcept C21547065 @default.
- W2067863352 hasConcept C2777132085 @default.
- W2067863352 hasConcept C2777166614 @default.
- W2067863352 hasConcept C2777289219 @default.
- W2067863352 hasConcept C2777504003 @default.
- W2067863352 hasConcept C2777995097 @default.
- W2067863352 hasConcept C38441620 @default.
- W2067863352 hasConcept C55493867 @default.
- W2067863352 hasConcept C67705224 @default.
- W2067863352 hasConcept C86803240 @default.
- W2067863352 hasConceptScore W2067863352C104317684 @default.
- W2067863352 hasConceptScore W2067863352C178666793 @default.
- W2067863352 hasConceptScore W2067863352C181199279 @default.
- W2067863352 hasConceptScore W2067863352C185592680 @default.
- W2067863352 hasConceptScore W2067863352C18903297 @default.
- W2067863352 hasConceptScore W2067863352C21547065 @default.
- W2067863352 hasConceptScore W2067863352C2777132085 @default.
- W2067863352 hasConceptScore W2067863352C2777166614 @default.
- W2067863352 hasConceptScore W2067863352C2777289219 @default.
- W2067863352 hasConceptScore W2067863352C2777504003 @default.
- W2067863352 hasConceptScore W2067863352C2777995097 @default.
- W2067863352 hasConceptScore W2067863352C38441620 @default.
- W2067863352 hasConceptScore W2067863352C55493867 @default.
- W2067863352 hasConceptScore W2067863352C67705224 @default.
- W2067863352 hasConceptScore W2067863352C86803240 @default.
- W2067863352 hasIssue "16" @default.
- W2067863352 hasLocation W20678633521 @default.
- W2067863352 hasLocation W20678633522 @default.
- W2067863352 hasOpenAccess W2067863352 @default.
- W2067863352 hasPrimaryLocation W20678633521 @default.
- W2067863352 hasRelatedWork W1981974337 @default.
- W2067863352 hasRelatedWork W2009178916 @default.
- W2067863352 hasRelatedWork W2014821888 @default.
- W2067863352 hasRelatedWork W2023345403 @default.
- W2067863352 hasRelatedWork W2023410336 @default.
- W2067863352 hasRelatedWork W2045056806 @default.
- W2067863352 hasRelatedWork W2067863352 @default.
- W2067863352 hasRelatedWork W21376089 @default.
- W2067863352 hasRelatedWork W2408267492 @default.
- W2067863352 hasRelatedWork W285243989 @default.
- W2067863352 hasVolume "25" @default.
- W2067863352 isParatext "false" @default.
- W2067863352 isRetracted "false" @default.
- W2067863352 magId "2067863352" @default.
- W2067863352 workType "article" @default.