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• W2067939308 abstract "This study aimed at evaluating the functional activation and activating receptors expression on resting, short- and long-term NK and NK-like T cells from blood of ovarian neoplasia patients. Blood from patients with adnexal benign alterations (n = 10) and ovarian cancer (grade I–IV n = 14) were collected after signed consent. Effector cells activation was evaluated by the expression of the CD107a molecule. Short-term culture was conducted overnight with IL-2 and long-term culture for 21 days, by a method designed to expand CD56+ lymphocytes. Short-term culture significantly increased NK cells activation compared to resting NK cells (p < 0.05), however, the long-term procedure supported an even higher increase (p < 0.001). Resting NK-like T cells showed poor activation, which was not altered by the culture procedures. The long-term culture effectively increased the expression of the activating receptors on NK and NK-like T cells, either by increasing the number of cells expressing a given receptor and/or by up-regulating their expression intensity. As a conclusion, the long-term culture system employed, resulted in a high number of functional NK cells. The culture system was particularly efficient on the up-regulation of NKp30 and DNAM-1 receptors on NK cells." @default.
• W2067939308 created "2016-06-24" @default.
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• W2067939308 date "2014-08-01" @default.
• W2067939308 modified "2023-10-01" @default.
• W2067939308 title "Up-regulation of DNAM-1 and NKp30, associated with improvement of NK cells activation after long-term culture of mononuclear cells from patients with ovarian neoplasia" @default.
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• W2067939308 doi "https://doi.org/10.1016/j.humimm.2014.05.010" @default.
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