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- W2067955914 abstract "An azo dye technique is described for the fine-structural localization of acid hydrolases. Enzymatically released primary reaction product, an insoluble substituted naphthol, is coupled with ρ-nitrobenzene diazonium-tetrafluoborate to produce a dense particulate azo dye. Simultaneous and post-coupling procedures are employed. The method is potentially applicable to all acid hydrolases and is free from the artifacts which beset the commonly used lead salt methods. The localization of β-glucuronidase and acid phosphatase in rat and slug liver is specifically dealt with here. Lysosomal and cytoplasmic sources of enzyme activity are revealed." @default.
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- W2067955914 date "1971-08-01" @default.
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- W2067955914 title "A high resolution technique for the fine-structural localization of acid hydrolases" @default.
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- W2067955914 doi "https://doi.org/10.1111/j.1365-2818.1971.tb02358.x" @default.
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