FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2067960358> ?p ?o ?g. }

• W2067960358 abstract "Interleukin-1 beta (IL-1 beta) causes rat islet cell dysfunction through mechanisms that involve inducible nitric oxide synthase (iNOS). However, IL-1 beta also activates several lipid pathways, including those generating phosphatidic acid (PA). Lisofylline (LSF), a water-soluble, nontoxic, selective inhibitor of the PA-1 alpha subspecies, which is stimulated by IL-1 beta and tumor necrosis factor-alpha, has been shown to prevent cytokine-induced cytotoxicity in in vivo animal models. To evaluate the effect of LSF on acute IL-1 beta-induced islet dysfunction, rat islets were exposed to IL-1 beta (0.1 ng/ml) with or without LSF (100 microM) for 24 h, followed by 25 mM glucose (G) stimulation, measurement of rat insulin by RIA, and calculation of the insulin secretion rate. In other experiments, rat islets were precultured for 48 h, then treated for 48 h in 25 mM G with or without IL-1 beta (0.1 ng/ml) and LSF (400 microM), and aliquots of medium were removed at 0, 24, and 48 h for measurement of rat insulin. In addition, islets were exposed to 25 mM G with or without IL-1 beta and LSF, lipids were then extracted, and PA subspecies were identified by TLC and mass spectroscopy, and quantitated using normal phase HPLC. Islets were also exposed to IL-1 beta with or without LSF, and Western immunoblots were performed to evaluate the effect of LSF on iNOS protein expression. IL-1 beta caused a 44% decrease in islet G-stimulated insulin secretion compared to that in untreated islets (P < 0.0005), which was totally reversed by LSF. In addition, IL-1 beta decreased the G-stimulated medium insulin content by 75% at 24 h (P = 0.0004) and 86% at 48 h compared to that in control islets (P < 0.0001). LSF-treated islets maintained 70% of medium insulin content at 24 h (P = 0.11) and 50% at 48 h (P < 0.0001) compared to control islets. HPLC quantitation of PA-1 alpha extracted from islets treated with IL-1 beta alone showed an approximately 15-fold increase over the PA-1 alpha content of islets treated with IL-1 beta and LSF. IL-1 beta-induced expression of iNOS was unchanged with the addition of LSF. These results suggest that LSF is effective in reducing IL-1 beta-induced islet dysfunction, thus supporting the role of lipid mediators such as PA in cytokine-induced islet toxicity." @default.
• W2067960358 created "2016-06-24" @default.
• W2067960358 creator A5020369383 @default.
• W2067960358 creator A5028576482 @default.
• W2067960358 creator A5041947832 @default.
• W2067960358 creator A5086802524 @default.
• W2067960358 date "1996-11-01" @default.
• W2067960358 modified "2023-09-24" @default.
• W2067960358 title "Lisofylline, an inhibitor of unsaturated phosphatidic acid generation, ameliorates interleukin-1 beta-induced dysfunction in cultured rat islets." @default.
• W2067960358 cites W103856407 @default.
• W2067960358 cites W1516715449 @default.
• W2067960358 cites W1517806507 @default.
• W2067960358 cites W1561813443 @default.
• W2067960358 cites W158558005 @default.
• W2067960358 cites W1605154807 @default.
• W2067960358 cites W1967738557 @default.
• W2067960358 cites W1970732641 @default.
• W2067960358 cites W2000919877 @default.
• W2067960358 cites W2002518981 @default.
• W2067960358 cites W2013300862 @default.
• W2067960358 cites W2021276343 @default.
• W2067960358 cites W2022336683 @default.
• W2067960358 cites W2031663707 @default.
• W2067960358 cites W2033977072 @default.
• W2067960358 cites W2040556202 @default.
• W2067960358 cites W2042038839 @default.
• W2067960358 cites W2043024227 @default.
• W2067960358 cites W2064035247 @default.
• W2067960358 cites W2072086410 @default.
• W2067960358 cites W2079994629 @default.
• W2067960358 cites W2084534198 @default.
• W2067960358 cites W2095138431 @default.
• W2067960358 cites W2107073863 @default.
• W2067960358 cites W2114730229 @default.
• W2067960358 cites W2126544381 @default.
• W2067960358 cites W2143182465 @default.
• W2067960358 cites W2168526937 @default.
• W2067960358 cites W2262548600 @default.
• W2067960358 cites W2420458010 @default.
• W2067960358 doi "https://doi.org/10.1210/endo.137.11.8895359" @default.
• W2067960358 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8895359" @default.
• W2067960358 hasPublicationYear "1996" @default.
• W2067960358 type Work @default.
• W2067960358 sameAs 2067960358 @default.
• W2067960358 citedByCount "18" @default.
• W2067960358 countsByYear W20679603582012 @default.
• W2067960358 countsByYear W20679603582015 @default.
• W2067960358 countsByYear W20679603582017 @default.
• W2067960358 countsByYear W20679603582018 @default.
• W2067960358 countsByYear W20679603582021 @default.
• W2067960358 countsByYear W20679603582022 @default.
• W2067960358 crossrefType "journal-article" @default.
• W2067960358 hasAuthorship W2067960358A5020369383 @default.
• W2067960358 hasAuthorship W2067960358A5028576482 @default.
• W2067960358 hasAuthorship W2067960358A5041947832 @default.
• W2067960358 hasAuthorship W2067960358A5086802524 @default.
• W2067960358 hasBestOaLocation W20679603581 @default.
• W2067960358 hasConcept C126322002 @default.
• W2067960358 hasConcept C134018914 @default.
• W2067960358 hasConcept C165220095 @default.
• W2067960358 hasConcept C185592680 @default.
• W2067960358 hasConcept C199360897 @default.
• W2067960358 hasConcept C2776174256 @default.
• W2067960358 hasConcept C2776828364 @default.
• W2067960358 hasConcept C2778690821 @default.
• W2067960358 hasConcept C2779306644 @default.
• W2067960358 hasConcept C41008148 @default.
• W2067960358 hasConcept C71924100 @default.
• W2067960358 hasConcept C74172505 @default.
• W2067960358 hasConcept C86803240 @default.
• W2067960358 hasConceptScore W2067960358C126322002 @default.
• W2067960358 hasConceptScore W2067960358C134018914 @default.
• W2067960358 hasConceptScore W2067960358C165220095 @default.
• W2067960358 hasConceptScore W2067960358C185592680 @default.
• W2067960358 hasConceptScore W2067960358C199360897 @default.
• W2067960358 hasConceptScore W2067960358C2776174256 @default.
• W2067960358 hasConceptScore W2067960358C2776828364 @default.
• W2067960358 hasConceptScore W2067960358C2778690821 @default.
• W2067960358 hasConceptScore W2067960358C2779306644 @default.
• W2067960358 hasConceptScore W2067960358C41008148 @default.
• W2067960358 hasConceptScore W2067960358C71924100 @default.
• W2067960358 hasConceptScore W2067960358C74172505 @default.
• W2067960358 hasConceptScore W2067960358C86803240 @default.
• W2067960358 hasLocation W20679603581 @default.
• W2067960358 hasLocation W20679603582 @default.
• W2067960358 hasOpenAccess W2067960358 @default.
• W2067960358 hasPrimaryLocation W20679603581 @default.
• W2067960358 hasRelatedWork W2024312973 @default.
• W2067960358 hasRelatedWork W2031301348 @default.
• W2067960358 hasRelatedWork W2039773086 @default.
• W2067960358 hasRelatedWork W2054500001 @default.
• W2067960358 hasRelatedWork W2072849820 @default.
• W2067960358 hasRelatedWork W2092346917 @default.
• W2067960358 hasRelatedWork W2129754316 @default.
• W2067960358 hasRelatedWork W2156180953 @default.
• W2067960358 hasRelatedWork W2402098708 @default.
• W2067960358 hasRelatedWork W2410681479 @default.
• W2067960358 isParatext "false" @default.
• W2067960358 isRetracted "false" @default.
• W2067960358 magId "2067960358" @default.

• next