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- W2068024828 abstract "The fluorescence spectra of subtilisin Carlsberg (STC) and subtilisin BPN′ (STB) were measured in aqueous–organic mixed solvents over a wide range of solvent composition. The tryptophan fluorophores were used as probes of the environmental polarity, and the structural changes of the enzymes were detected as changes of the difference in the emission wavelength between the enzymes and N-acetyl-L-tryptophan ethyl ester (Δλem). It was found that Δλem was well correlated with the catalytic activity of the enzymes, as expressed by the hydrolysis rate of N-acetyl-L-tyrosine ethyl ester. For example, the characteristic dependency of the activity of STC on the solvent composition in acetonitrile–water was closely related to the change in Δλem. Similar correlations between Δλem and the catalytic activity were observed for subtilisins in aqueous 1,4-dioxane, THF, DMF, DMA, and DMSO solutions with high correlation coefficients, except for STB in water–DMF and –DMA. The results are discussed in terms of the structural modification of the enzymes in these aqueous–organic media." @default.
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- W2068024828 date "1994-10-01" @default.
- W2068024828 modified "2023-10-11" @default.
- W2068024828 title "Fluorescence Spectroscopic Study of Subtilisins as Relevant to Their Catalytic Activity in Aqueous–Organic Media" @default.
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- W2068024828 doi "https://doi.org/10.1246/bcsj.67.2819" @default.
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