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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068044379> ?p ?o ?g. }

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• W2068044379 abstract "A 5' truncated caprine (ca) κ-casein-encoding gene (κCas) was fused to the 3′ end of a 3′ truncated ca βCas. The βCas form comprised the 0.8-kb 3′ end of intron 2, the remaining part of the transcription unit containing codons -2 to stop 172, and 0.43 kb of the 3' flanking region. The βCas form comprised a 3-kb 5′ flanking region and the 5′ end of the transcription unit terminating 69 bp dowstream from exon 2 which encodes the 15-amino-acid (aa) signal peptide and the first 2 aa of mature βCas. The resulting hybrid gene driven by the βCas promoter was expressed in all eight lines of transgenic mice investigated, although at different levels. In two lines, the yield of recombinant (re-) κCas was ≥ 3 mg/ml of milk. The stage- and mammary tissue-specific expression was similar to that of endogenous βCas. The re-κCas differed from its goat milk counterpart by the occurrence of four extra aa at the N-terminal end, indicating that the signal peptidase released the βCas signal peptide. According to sedimentation analyses of murine milk containing ≥ 3 mg re-κCas/ml, the latter essentially occurred in micelles. Preliminary comparative assays of the behavior of αs1Cas-κCas and αs1Cas-reκCas mixtures upon incremental addition of Ca2+ showed that re-κCas had the capacity to protect αs1Cas against Ca2+-induced precipitation in forming stable micelles. In contrast, the expression of a reconstituted ca κCas gene was in the range of a few μg re-κCas/ml of milk in the five transgenic lines investigated." @default.
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• W2068044379 date "1995-01-01" @default.
• W2068044379 modified "2023-10-14" @default.
• W2068044379 title "High-level, stage- and mammary-tissue-specific expression of a caprine κ-casein-encoding minigene driven by a β-casein promoter in transgenic mice" @default.
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• W2068044379 doi "https://doi.org/10.1016/0378-1119(95)00405-u" @default.
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