Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068046817> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2068046817 abstract "The protein and glycoprotein composition of a sucrose gradient fraction from vaccinia infected cells treated with rifampicin was studied. This particulate fraction contained cytoplasmic membranes and pleomorphic membranous structures. The glycoproteins (89, 42 and 20-23 kDa, respectively) were identified as the same glycoproteins that are found in plasma membranes of infected cells and the envelope of extracellular enveloped vaccinia (EEV). These glycoproteins could be solubilized by 0.1% NP-40. The Golgi membrane associated 41K acylated vaccinia protein was also NP-40 soluble. In contrast, most particulate fraction proteins (125, 100, 86, 65, 41, 39, 31, 27, 25, 14 and 12.5 kDa) with the exception of the 33 and 29 kDa proteins remained essentially insoluble after NP-40 treatment. The 86 and 65 kDa proteins are the rifampicin inhibited precursors to INV core proteins while the 33 and 29 kDa proteins are INV surface proteins. Twelve proteins behaved like their respective comigrating INV proteins when extracted with NP-40 and 2ME. Electron microscopy showed that a centrifuged sediment from NP-40 treated cells contained pleomorphic protein containing membranous structures that we have called rifampicin bodies. We conclude that (1) the major glycoproteins found in the particulate fraction from sucrose gradients are vaccinia glycoproteins residing in cytoplasmic membranes while (2) the major non-glycosylated proteins are components of the rifampicin bodies and that (3) the rifampicin bodies represent an intermediate in the morphogenetic process leading to mature INV." @default.
- W2068046817 created "2016-06-24" @default.
- W2068046817 creator A5005253217 @default.
- W2068046817 creator A5078566025 @default.
- W2068046817 date "1990-08-01" @default.
- W2068046817 modified "2023-10-02" @default.
- W2068046817 title "The polypeptide composition of vaccinia-infected cell membranes and rifampicin bodies" @default.
- W2068046817 cites W1576056823 @default.
- W2068046817 cites W1604071914 @default.
- W2068046817 cites W1616543567 @default.
- W2068046817 cites W1905305740 @default.
- W2068046817 cites W1905405046 @default.
- W2068046817 cites W1976192184 @default.
- W2068046817 cites W1996884863 @default.
- W2068046817 cites W2006132691 @default.
- W2068046817 cites W2013047014 @default.
- W2068046817 cites W2014622451 @default.
- W2068046817 cites W2025743844 @default.
- W2068046817 cites W2027393724 @default.
- W2068046817 cites W2034277598 @default.
- W2068046817 cites W2045005947 @default.
- W2068046817 cites W2072347896 @default.
- W2068046817 cites W2100837269 @default.
- W2068046817 cites W2110968011 @default.
- W2068046817 cites W2122916550 @default.
- W2068046817 cites W2155725626 @default.
- W2068046817 doi "https://doi.org/10.1016/0168-1702(90)90077-o" @default.
- W2068046817 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2220070" @default.
- W2068046817 hasPublicationYear "1990" @default.
- W2068046817 type Work @default.
- W2068046817 sameAs 2068046817 @default.
- W2068046817 citedByCount "4" @default.
- W2068046817 crossrefType "journal-article" @default.
- W2068046817 hasAuthorship W2068046817A5005253217 @default.
- W2068046817 hasAuthorship W2068046817A5078566025 @default.
- W2068046817 hasConcept C104317684 @default.
- W2068046817 hasConcept C108625454 @default.
- W2068046817 hasConcept C119062480 @default.
- W2068046817 hasConcept C144647389 @default.
- W2068046817 hasConcept C1491633281 @default.
- W2068046817 hasConcept C153911025 @default.
- W2068046817 hasConcept C190062978 @default.
- W2068046817 hasConcept C2781356689 @default.
- W2068046817 hasConcept C40767141 @default.
- W2068046817 hasConcept C41625074 @default.
- W2068046817 hasConcept C55493867 @default.
- W2068046817 hasConcept C86803240 @default.
- W2068046817 hasConceptScore W2068046817C104317684 @default.
- W2068046817 hasConceptScore W2068046817C108625454 @default.
- W2068046817 hasConceptScore W2068046817C119062480 @default.
- W2068046817 hasConceptScore W2068046817C144647389 @default.
- W2068046817 hasConceptScore W2068046817C1491633281 @default.
- W2068046817 hasConceptScore W2068046817C153911025 @default.
- W2068046817 hasConceptScore W2068046817C190062978 @default.
- W2068046817 hasConceptScore W2068046817C2781356689 @default.
- W2068046817 hasConceptScore W2068046817C40767141 @default.
- W2068046817 hasConceptScore W2068046817C41625074 @default.
- W2068046817 hasConceptScore W2068046817C55493867 @default.
- W2068046817 hasConceptScore W2068046817C86803240 @default.
- W2068046817 hasLocation W20680468171 @default.
- W2068046817 hasLocation W20680468172 @default.
- W2068046817 hasOpenAccess W2068046817 @default.
- W2068046817 hasPrimaryLocation W20680468171 @default.
- W2068046817 hasRelatedWork W1983561321 @default.
- W2068046817 hasRelatedWork W1983983855 @default.
- W2068046817 hasRelatedWork W2005163569 @default.
- W2068046817 hasRelatedWork W2029739670 @default.
- W2068046817 hasRelatedWork W2068046817 @default.
- W2068046817 hasRelatedWork W2079756626 @default.
- W2068046817 hasRelatedWork W2084905482 @default.
- W2068046817 hasRelatedWork W2408866861 @default.
- W2068046817 hasRelatedWork W2510687505 @default.
- W2068046817 hasRelatedWork W3178209818 @default.
- W2068046817 isParatext "false" @default.
- W2068046817 isRetracted "false" @default.
- W2068046817 magId "2068046817" @default.
- W2068046817 workType "article" @default.